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OBJECTIVE: To establish a method for simultaneous determination of multiple metal elements in Millettia speciosa Champ. METHODS: Eight kinds of metal elements, i.e., Fe, Mn, Cu, Zn, Pd, Cd, As, and Se in Millettia speciosa Champ from different origins were determined by microwave digestion and ICP-MS. RESULTS: The contents of Fe, Mn, Cu, Zn, Pd, Cd, As, and Se in Millettia speciosa Champ from different origins were slightly different, the contents of Fe, Mn, Cu, and Zn were all above 1 mg·kg-1, while the contents of Pd, Cd, As and Se were mostly less than 1 mg·kg-1. The linear correlation coefficient of each metal element was 0.999 2-1.000 0, the average recovery was 87%-110.8%, and the RSDs of the reproducibility test was less than 10%. CONCLUSION: A microwave digestion and ICP-MS method for simultaneous determination of multiple metal elements in Chinese medicine Millettia speciosa Champ is established. The method is simple, sensitive, precise and repeatable, which can be used to determine the contents of metals in Millettia speciosa Champ.
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Objective To explore the mechanism of immunoregulation by investigating the effects of polysaccharides from Millettia speciosa Champ (MSC) on proliferation of spleen lymphocyte and secretion of cytokine in mice.Methods The effects of MSC polysaccharides on Con A-induced spleen T lymphocyte proliferation were determined by MTT.The contents of TNF-α, IL-6 and PGE2 were determined by ELISA.Results The Con A-induced T lymphocyte proliferation was significantly increased by MSC polysaccharides at the concentrations from 50 to 200 μg·mL-1.Coupled with TNF-α and IL-6 were significantly increased while PGE2 was significantly decreased.Conclusion MSC polysaccharides could increase proliferation of spleen lymphocyte and enhance the immune responses by increasing the secretion of TNF-α and IL-6 in mice.
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Objective To compare the immunoregulatory effects of Radix Millettia Speciosa (RM Speciosa) and Radix Millettia Championi (RM Championi)on immunosuppressed mice.Methods Kunming mice were randomly divided into normal control group,CTX model group,LMS positive group,RM Speciosa groups and RM Championi groups (20,10 and 5 g/kg).The mice were treated respectively with drug or NS once a day for consecutive 20 days.Mice in the other groups were injected intraperitoneally with CTX at days 8,10 and 12 to establish immunosuppressed mice model except the normal group.The changes of body weight,immune organ weight,white blood cell (WBC)number,carbon particle clearance capability of macrophages and delayed type hypersensitivity (DTH)of mice in all groups were determined and compared.Results Compared with that in CTX group,the WBC number was significantly increased (P0.05).Conclusion Both RM Speciosa and RM Championi can improve the immune function of CTX-induced immunosuppressed mice,and RM Speciosa is slightly superior to RM Championi in improving specific cellular immunity.
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Objective To study the chemical constituents from the roots of Millettia speciosa. Methods The chemical constituents were isolated and purified by silica gel, Sephadex LH-20, and ODS column chromatography. The structures were identified by means of spectral data. Results Fifteen compounds were isolated and identified as naringenin (1), liquiritigenin (2), garbanzol (3), 7-hydroxy-6,4′- dimethoxyisoflavone (4), calycosin (5), 2′,5′,7-trihydroxy-4′-methoxyisoflavone (6), 2′-hydroxybiochanin A (7), 6-methoxycalopogonium isoflavone A (8), demethylmedicarpin (9), 4,4′-dihydroxy-2′-methoxychalcone (10), 2′,4′-dihydroxy-4-methoxychalcone (11), rhododendrol (12), secoisolariciresinol (13), bisdihydrosiringenin (14), and polystachyol (15). Conclusion All compounds are obtained from this plant for the first time.
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Objective: To study the chemical constituents from Millettia speciosa. Methods: The compounds were isolated and purified by silica gel, Sephadex LH-20, ODS column chromatography, and recrystallization. The structures were identified using physicochemical and spectral data. Results: Thirteen compounds were isolated from M. speciosa and identified as docosanoic acid (1), tetracosane (2), octadecane (3), hexacosanoic acid (4), β-sitosterol acetate (5), β-sitosterol (6), syringin (7), maackiain (8), formononetin (9), ψ-baptigenin (10), rotundic acid (11), pedunculoside (12), and daucosterol (13). Conclusion: Compounds 5, 7, and 10-12 are obtained from this plant for the first time.
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Objective: To study the chemical constituents from the roots of Millettia speciosa (Leguminosae). Methods: Compounds in the 95% ethanol extract from the air-dried roots of M. speciosa were isolated by chromatography on silica gel column together with recrystallization, and their structures were identified by their physicochemical characteristics and spectral features. Results: Sixteen components were isolated from the air-dried roots of M. speciosa and identified as 7-oxo-β-sitosterol (1), aurantiamide acetate (2), shionone (3), maleic acid (4), psoralen (5), N-methylcytisine (6), lupeol caffeate (7), bisdemethoxycurcumin (8), vanillic acid (9), syringic acid (10), 6-methoxydihdyrosanguinarine (11), glycyrrhizic acid (12), (E)-3, 3'-dimethoxy-4, 4'-dihydroxystilbene (13), schisandrol B (14), 7-hydroxylathyrol (15), and nardosinone (16). Conclusion: All the compounds are isolated from this plant for the first time, and compounds 3, 7, 8, 11, and 13-16 are isolated from the plants of Leguminosae for the first time, compounds 2, 4-6, 9, 10 are isolated from the plants of Millettia Wight et Arn. for the first time.