Classification and phylogenetic analysis of the genus Mobiluncus based on matrix-assisted laser de-sorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and whole genome sequen-cing / 中华微生物学和免疫学杂志

Objective To identify Mobiluncus species with matrix-assisted laser desorption/ioniza-tion time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing and to analyze anti-biotic resistance genes and phylogenetic relationships among Mobiluncus species with whole genome sequen-cing. Methods Twenty-five Mobiluncus strains were isolated after anaerobic culture of 65 vaginal secretion samples of patients with bacterial vaginosis (BV) and identified to species level by MALDI-TOF MS and 16S rRNA gene sequencing. The whole genome DNA of each strain was extracted for next-generation sequencing. SPAdes was used to assembly genomes. Resistance genes were searched in ARGD database. The core ge-nome of all isolates was analyzed by Harvest to construct phylogenetic tree. Results MALDI-TOF MS could only identify Mobiluncus curtisii, while 16S rRNA gene sequencing could identify both Mobiluncus mulieris and Mobiluncus curtisii. Results of the whole genome sequencing showed that tetracycline resistance gene tet ( o) and macrolides resistance gene erm(x) were the two predominant acquired resistance genes of Mobilun-cus with a positive rate of 84. 7% and 61. 5% respectively. Intra-species relationships of the two Mobiluncus species were close, but a distant phylogenetic relationship was found between the two species. Conclusion This study shows that MALDI-TOF MS can't be used to identify Mobiluncus mulieris at present. Mobiluncus strains have potential resistance to tetracycline and macrolides. Intra-species evolution of Mobiluncus is slow, which indicates that there is no growing trend towards new species for the time being.

Perfil clínico e microbiológico de mulheres com vaginose bacteriana / Clinical and microbiological profile of women with bacterial vaginosis

OBJETIVO: estudar o perfil clínico e microbiológico de mulheres portadoras de vaginose bacteriana participantes de um ensaio clínico randomizado, duplamente mascarado, que comparou aroeira e metronidazol, em uso vaginal, para tratamento do corrimento genital. MÉTODOS: o estudo constitui-se em uma série de casos de 277 mulheres portadoras de vaginose bacteriana diagnosticada, concomitantemente, pelos critérios de Amsel e Nugent, selecionadas a partir de um total de 462 recrutadas, utilizando as informações colhidas antes da intervenção. A análise dos dados foi efetuada utilizando-se o programa Epi-Info 3.32. Para comparar as frequências dos desfechos entre os grupos de intervenção, foi utilizado o teste do χ2 e foi calculada a razão de risco e o intervalo de confiança a 95 por cento. Foi feita análise por intenção de tratar. Além dos parâmetros de diagnósticos, foram também colhidas cultura do conteúdo vaginal e uma citologia de Papanicolaou. RESULTADOS: entre as queixas clínicas, as mais frequentes foram o corrimento genital, observado em 206 participantes (74,4 por cento) e o odor de peixe da secreção vaginal, que ocorreu em 68,6 por cento dos casos (190 pacientes). Dentre os critérios clínicos de diagnósticos, a presença de clue-cells foi positiva em 275 mulheres (99,3 por cento), o teste de Whiff positivo apareceu em 266 participantes (96,0 por cento), seguido do pH >4,5, que ocorreu em 92,8 por cento dos casos e da presença de corrimento fluido e acinzentado, citado por 206 participantes (74,4 por cento). Com relação ao critério de Nugent, a mediana dos escores foi o valor 8,0. As culturas de conteúdo vaginal permitiram a identificação de Gardnerella vaginalis em 96,8 por cento e de Mobiluncus, em 53,1 por cento dos casos. Apenas uma terça parte dos exames mostrou a presença de Lactobacillus (89 mulheres - 32,1 por cento). Houve crescimento de fungos em culturas de 14 participantes (5,1 por cento). Na maior parte dos casos, os ...

PURPOSE: to study the clinical and microbiological profile of women with bacterial vaginosis participating in a randomized, double-blind clinical trial, which compared the vaginal use of preparations from red pepper tree and metronidazole for the treatment of genital discharge. METHODS: the study was conducted on a series of 277 women with bacterial vaginosis concomitantly diagnosed by the criteria of Amsel and Nugent, selected from a total of 462 recruited patients using the information obtained before intervention. Data were analyzed with the Epi-Info 3.32 software. In order to compare the outcomes frequencies between the intervention groups, the χ2 test was used and the risk ratio and 95 percent confidence interval were calculated. The intention to treat analysis was performed. In addition to the determination of diagnostic parameters, the culture of vaginal content and a Papanicolaou cytology test were also performed. RESULTS: the most frequent clinical complaints were genital discharge, observed in 206 participants (74.4 percent) and the fish odor of the vaginal secretion, which occurred in 68.6 percent of the cases (190 patients). Among the diagnostic clinical criteria, the presence of clue-cells was positive in 275 women (99.3 percent), the Whiff test, in 266 (96.0 percent), followed by pH >4.5, which occurred in 92.8 percent of the cases, and by the presence of fluid grayish discharge reported by 206 participants (74.4 percent). Regarding the Nugent criterion, the median score was 8.0. Culture of the vaginal content permitted the identification of Gardnerella vaginalis in 96.8 percent of cases and of Mobiluncus in 53.1 percent. Only one third of the exams showed the presence of Lactobacillus (89 women - 32.1 percent). Fungal growth occurred in the cultures of 14 participants (5.1 percent). In most cases, culture revealed the presence of Corynebacterium (94.2 percent), Gram-positive cocci (98.2 percent), as well as Gram-positive ...

Prevalência de gardnerella e mobiluncus em exames de colpocitologia em Tomé-Açu, Pará / Prevalence of gardnerella and mobiluncus in vaginal cytology examinations in Tome-Açu-Pará

Objetivo: a Gardnerella vaginalis, isoladamente ou associada ao Mobiluncus sp., é um dos principais agentes causadores de infecções em mulheres em idade reprodutiva, seja pela falta de hábitos de higiene adequados, número de parceiros sexuais ou desequilíbrios da microflora vaginal. Assim, buscou-se verificar a prevalência de G vaginalis e/ou Mobiluncus sp., associadas às condições sócio-culturais em mulheres atendidas no Hospital de Quatro Bocas de Tomé-Açu - Pará, através de exame preventivo de câncer de colo do útero. Método e Resultados: analisados materiais cervico-vaginais e informações sócio-culturais de 156 mulheres, com faixas etárias de 17 e 73 anos, período de outubro de 2003 a julho de 2004, para o diagnóstico de prevenção de câncer ginecológico. Todas as amostras foram processadas de modo convencional e analisadas no Laboratório de Citopatologia da Universidade Federal do Pará por três observadores (estudo cego). Como resultados verificou-se que das 156 mulheres pesquisadas , a maior prevalência estava associada à G vaginalis (48,15%), faixa etária de 21 a 30 anos, ou pela associação G vaginalis e Mobiluncus sp (36,36%). Em relação ao grau de escolaridade e ocupação, a maior prevalência ocorreu em mulheres com r grau incompleto (47,37%), com atividades do lar (21,05%) e comércio (22,81%). Conclusões: estes dados reforçam pesquisas que relacionam a prevalência de G vaginalis e/ou Mobiluncus sp, em mulheres com vida sexual e menor grau de escolaridade.

Objective: gardnerella vaginalis, alone or associated to Mobiluncus sp., is one of the main etiological agents ofinfection in women of reproductive age, due to lack of appropriate hygiene habits, number of sexual partners ar imbalance of the vaginal microbiota. Therefore, our objective was to assess the prevalence of bacterial vaginosis caused by G vaginalis and/or Mobiluncus sp., and associate this to the sociocultural conditions in women attended ai Hospital de Quatro Bocas in Tomé-Açu - Pará - Brazil, through gynecological cancer screening. Methods and Results: cervico-vaginal smears and socio-cultural information of 156 women aged 17 to 73 were analyzedfor diagnosis and prevention of gynecological cancer from October 2003 to July 2004. Ali samples were processed in the conventional way and analyzed at Universidade Federal do Pará's Laboratory of Cytopathology by three observers (a blind study). Among ali 156 women, the largest prevalence was associated to G vaginalis (48,15%) in 21 to 30- year-old women, ar to the association of G vaginalis and Mobiluncus sp (36,36%). As for the occupation and instruction degree, the infection was most prevalent in women with incomplete primary school (47,37%), housewives (21,05%) and market workers (22,81 %). Conclusions: our data reaffirm previous researches that relate the prevalence ofbacterial vaginosis caused by G vaginalis and /or Mobiluncus sp. to low instruction degree in sexually active women.