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Objective:To investigate the effectiveness and safety of soothing moisturizing repair cream on acne depressed scar exfoliative fractional laser wound repair.Methods:From October 2018 to June 2020, the Department of Dermatology, Qingdao Haici Hospital Affiliated to Qingdao University took 33 patients with acne depressed scars as the research object, including 8 males and 25 females, aged from 20 to 36 years (29.6±8.6) years. The left and right face comparison method was adopted. After laser operation, the trial side was given a soothing moisturizing repair cream, and the control side was given a placebo. By collecting the patient's facial pictures and objective skin data before and after the laser operation, 1 h, 1 d, 3 d, 7 d, 21 d, and combined with the researcher's semi-subjective evaluation and patient's subjective evaluation the wound skin reaction and wound healing were observed.Results:At 1, 3, 7, 21 days after laser operation, the skin water content of the test side was higher than that of the control side ( P<0.05), and the skin water loss was lower than the control side ( P<0.05); at 3, 7, 21 days, the skin pigment of the test side was lower than the control side ( P<0.05); at 3, 7 d, the test side skin erythema index was lower than the control side ( P<0.05); at 1, 3, 7 d, the test side wound skin erythema, edema, dryness and tightness, etc. were better than the control side ( P<0.05). The duration of pain, crusting time, scab removal time, and complete healing time of the wound on the test side were shorter than those on the control side ( P<0.05). The patient's satisfaction with the moisturization and comfort of the nursing products on the trial side was better than that on the control side ( P<0.05). Conclusions:There is no adverse reaction to the soothing moisturizing repair cream after laser surgery, which can better inhibit skin inflammation, reduce post-inflammatory pigmentation, promote skin healing, and help repair the wound after laser surgery.
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Objective:To observe the efficacy of anti-sensitive moisturizing tolerance-extreme cream combined with isotretinoin in the treatment of severe acne.Methods:Fifty patients with severe acne were selected in the Dermatology Clinic of the Third People＇s Hospital of Hangzhou from November 2018 to July 2019. They were randomly divided into the experimental group of 25 cases and the control group of 25 cases. The experimental group was treated with anti-sensitive moisturizing tolerance-extreme cream combined with isotretinoin orally. The control group was treated with isotretinoin orally alone. Before and after treatment for 56 days, lactate score, skin cuticle hydration (SCH), transepidermal water loss (TEWL) and skin physiological indexes were measured.Results:After 56 days of treatment, the TEWL and SCH of the control group were 15.75±3.31 and 10.13±3.62, the TEWL and SCH of the experimental group were 12.17±3.61 and 28.07±3.17, respectively; the difference was statistically significant ( T was 3.610 and 12.398, P was 0.002 and 0.000, respectively). The volume and depth of cyst nodule, scar depression, skin roughness, absolute value and area of erythema in the experimental group were significantly lower than those in the control group ( T was 2.280, 1.676, 2.332, 1.508, 4.813 and 3.637; P was 0.031, 0.011, 0.027, 0.040, 0.000 and 0.001, respectively). Conclusions:Anti-sensitive moisturizing tolerance-extreme cream combined with isotretinoin has a good effect on severe acne and it can reduce the barrier damage and other adverse reactions.
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OBJECTIVE: To investigate an efficient hydrating facial mask with olive oil (OL)/vitamin E succinate(VES) as the oil phase(O), and with hyaluronic acid, collagen, sodium alginate, arbutin, allantoin as the active ingredients, using a low concentration of surfactant(S) and alcohol-free microemulsion gel as the carrier. METHODS By investigating the effect of VES and co-surfactant (CoS) propylene glycol on microemulsion region, the microemulsion formulation was screened. The microemulsion characteristics of the facial mask were characterized by transmission electron microscopy and Malvern particle size determination. The moisturizing effect and transdermal absorption capacity of the facial mask were evaluated using a commercially available unifon mask as the reference substance. RESULTS Through the compounding of OL and VES, the microemulsion area of OL was increased, and microemulsion range was largest when the mass ratio of OL to VES was 6∶1.The use of the co-surfactant(propylene glycol) reduced the OL microemulsion area. The microemulsion with O/S mass ratio of 4/6 and water content of 85% was selected as the carrier, and the obtained droplet of the facial mask was round, the average particle size was (58.83±0.79) nm, and the PDI was (0.271±0.001), which were in line with the characteristics of microemulsion. Compared with commercial unifon mask, the moisturizing effect of the self-made microemulsion gel facial mask increased by 20.37%. The steady-state infiltration rates of 1% arbutin from the facial mask through the isolated cavy skin was 0.305 mg•cm-2•h-1, which was 4.29 times higher than that from the marketed unifon mask. This product was safe and stable, and meets the sensory and physicochemical indexes of the national light industry standard QB/T2872-2007. CONCLUSION The facial mask with OL/VES microemulsion gel as the carrier can improve the hydrating effect and the transdermal absorption of the active substance, it is expected to be promoted and developed.
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OBJECTIVE： To prepare the Peppermint oil moisturizing microemulsion for nasal mucosa and survey its mucosal adhesion and cilia toxicity. METHODS： The polyoxyethylene hydrogenated castor oil was used as emulsifier to prepare the Peppermint oil moisturizing microemulsion for nasal mucosa， and the preparation technology was optimized on the basis of comprehensive score by orthogonal design. The microemulsion was characterized and the menthol content was determined by GC. The mucosal adhesion was evaluated by measuring the transport rate by cilia in vivo， and the cilia toxicity of microemulsion was evaluated by measuring the sustained movement time of cilia in vitro. RESULTS： The optimal preparation technology of self-made microemulsion was to firstly disperse the peppermint oil and the emulsifier， then add anhydrous ethanol， edible glycerin and distilled water， and stir at 1 200 r/min for 2 h. The average contents of menthol in the three batches of the microemulsion were 2.682， 2.507 and 2.496 mg/mL （RSD＝2.89％，n＝3）， respectively. The cilia transport rates in vivo were （0.65±0.01）， （0.78±0.03）and （0.92±0.04） cm/min in high-dose， medium-dose， and low-dose groups of self-made microemulsion （2.561， 0.256， 0.128 mg/mL of menthol） respectively， which were significantly lower than normal saline group and compound menthol nasal droups （P＜0.05）. The cilia movement time in vitro were（206.7±4.9）， （226.0±13.5）， （269.3±12.9）min， which were significantly longer than sodium deoxycholate group （P＜0.05）. CONCLUSIONS： The preparation technology of self-made microemulsion is easy-to-handle and controllable in quality. The prepared microemulsion shows good mucosal adhesion without cilia toxicity．
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The purpose of this study was to evaluate the safety of Kalanchoe brasiliensis extract, followed by the development of an oil in water emulsion containing the K. brasiliensis leaves extract and evaluating its clinical moisturizing efficacy. The formulations containing sodium acrylates/ Beheneth-25 methacrylate Crosspolymer (and) hydrogenated polydecene (and) lauryl glucoside and 0.5% of extract were prepared. The extract was considered as non-irritating through skin irritant tests. The stability testing was carried out in different conditions for 90 days. The skin hydration was measured by capacitance measurement and transepidermal water loss using biophysical techniques. The results indicate that the formulation containing 0.5% of extract increased the hydration of the stratum corneum up to 5 h after application on the forearm. The transepidermal water loss was reduced when compared to the untreated area and placebo area. Therefore, we can conclude that the increased skin hydration and protection of barrier function can be attributed to the K. brasiliensis extract. This research presents a new raw material from the Brazilian Caatinga biome and shows its possible application in the development of cosmetic products.
Subject(s)
Wetting Agents/pharmacology , Kalanchoe/anatomy & histology , Emulsions , Plant Extracts/adverse effects , Cosmetic StabilityABSTRACT
Nas últimas décadas o conhecimento sobre a etiopatogenia da dermatite atópica (DA) avançou muito. Além da identificação dos principais agentes desencadeantes e/ou agravantes envolvidos na expressão clínica da DA, verificou-se ser a integridade da barreira cutânea um dos pontos fundamentais para a manutenção da homeostase da pele. Assim, no tratamento do paciente com DA, além da evitação dos agentes desencadeantes e/ou irritantes, o uso de hidratantes é parte fundamental, e acredita-se que tenha ação preventiva de surtos agudos. Além disso, a aquisição de agentes anti-inflamatórios de uso tópico tem permitido o controle de pacientes com formas leves a moderadas da DA. Embora tenham uso mais restrito, os agentes imunossupressores sistêmicos também têm sido empregados no tratamento de pacientes com DA grave ou refratária aos procedimentos habituais. Comenta-se também a imunoterapia alérgeno-específica como tratamento adjuvante da DA para alguns pacientes, sobretudo alérgicos aos ácaros e com manifestações respiratórias associadas. A aquisição de novos agentes, os imunobiológicos, também são apresentados à luz das evidências científicas e clínicas atuais. O presente guia prático de atualização em dermatite atópica abordagem terapêutica teve por objetivo rever os esquemas de tratamento disponíveis e empregados no acompanhamento de pacientes com DA, além de apresentar terapêuticas futuras, como os agentes imunobiológicos que em breve estarão à disposição para o tratamento de formas mais graves e/ou refratárias da DA.
Over the last few decades, knowledge of the etiopathogenesis of atopic dermatitis (AD) advanced greatly. The main triggering and/or aggravating factors involved in the clinical expression of AD have been identified, and cutaneous barrier integrity has been found to be key for the maintenance of skin homeostasis. Thus, when treating patients with AD, in addition to avoiding triggering and/ or irritating agents, recommending the use of skin moisturizers is paramount and believed to have a preventive action against acute outbreaks. Moreover, topical anti-inflammatory agents have allowed AD control in patients with mild to moderate forms of the disease. Although more restricted, systemic immunosuppressive agents have also been used in the treatment of patients with severe or refractory AD. Specific allergen immunotherapy is presented as a possible adjunctive treatment for AD in some patients, especially those allergic to mites and presenting associated respiratory manifestations. Finally, the use of new immunobiological agents is discussed in the light of the scientific and clinical evidence currently available. The objectives of this updated practical guide on atopic dermatitis treatment approach were to review the treatment regimens available and used in the follow-up of patients with AD and to present new therapies (e.g., immunobiological agents) that will soon be available for the treatment of more severe and/ or refractory forms of AD.
Subject(s)
Humans , Male , Female , History, 21st Century , Guidelines as Topic , Dermatitis, Atopic , Allergy and Immunology , Skin , Societies, Medical , Therapeutics , Wetting Agents , Cyclosporine , Adrenal Cortex Hormones , Calcineurin Inhibitors , Immunosuppressive Agents , ImmunotherapyABSTRACT
AIM: To observe the clinical effects of moisturizing eye decoction combined with artificial tears in the treatment of ophthalmoxerosis.METHODS: A randomized controlled clinical research method was employed.One hundred-sixty patients diagnosed with ophthalmoxerosis were equally divided into a treatment group and a control group.In the treatment group,patients were given one dose per day of the moisturizing eye decoction as well as artificial tear drops three times a day.The patients in the control group were treated with artificial tears drops three times a day.The course of treatment was 3mo.Dry-eye symptoms,tear break-up time(BUT) by the slit lamp,the time of tear secretion (Schirmers Ⅰ test),corneal fluorescein staining (FL),and curative effects were observed before treatment as well as in the first and third months after treatment.RESULTS: There were no significant differences between the two groups in terms of BUT,SⅠt,and FL before treatment (P>0.05).After treatment in the first and the third months,the BUT,SⅠt,FL,and curative effects were significantly different between the two groups (P<0.05).CONCLUSION: Moisturizing eye decoction combined with artificial tears in the treatment of ophthalmoxerosis can improve efficacy.
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PURPOSE: Ultraviolet (UV) irradiation decreases epidermal hydration, which is maintained by reduction of natural moisturizing factors (NMFs). Among various NMFs, free amino acids (AA) are major constituents generated by filaggrin degradation. This experiment was conducted to determine whether or not dietary supplementation of green tea extract (GTE) in UV-irradiated mice can improve epidermal levels of hydration, filaggrin, free AAs, and peptidylarginine deiminase-3 (PAD3) expression (an enzyme involved in filaggrin degradation). METHODS: Hairless mice were fed a diet of 1% GTE for 10 weeks in parallel with UV irradiation (group UV+1%GTE). As controls, hairless mice were fed a control diet in parallel with (group UV+) or without (group UV-) UV irradiation. RESULTS: In group UV+, epidermal levels of hydration and filaggrin were lower than those in group UV-; these levels increased in group UV+1% GTE to levels similar to group UV-. Epidermal levels of PAD3 and major AAs of NMF, alanine, glycine and serine were similar in groups UV- and UV+, whereas these levels highly increased in group UV+1% GTE. CONCLUSION: Dietary GTE improves epidermal hydration by filaggrin generation and degradation into AAs.
Subject(s)
Animals , Mice , Alanine , Amino Acids , Diet , Dietary Supplements , Epidermis , Glycine , Metabolism , Mice, Hairless , Serine , TeaABSTRACT
O maior problema ocasionado pelo ressecamento da mucosa nasal é a repercussão que gera sobre o movimento do muco, isto é, a diminuição hídrica leva a retenção de secreções. Vários estudos avaliam os efeitos colaterais durante o tratamento da isotretinoína e são divididos em dois grupos: mucocutâneos e sistêmicos. Neste sentido, o presente artigo visa analisar os efeitos mucocutâneos da isotretinoína, suas alterações e complicações na mucosa nasal e pulmonar.
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Objective To study mechanical properties of the microstructure of the articular cartilage with biological activity by nanoindentation tests under the condition of keeping the articular cartilage in water. Methods The different abilities to keep in situ mechanical properties of the articular cartilage with cold mosaic method or with moisturizing method were evaluated by experiments, and the mechanical properties of the microstructure of the articular cartilage in different kinds of solution used in moisturizing method were also obtained. Results The experiment showed that the elastic modulus on the cartilage surface layer with cold mosaic method was much higher than that with moisturizing method, and the elastic modulus on the cartilage surface and deep layer in chitosan and saline were much lower than that in distilled water. Conclusions The moisturizing method is better at preserving in situ mechanical properties and biological activity of the biological materials, and chitosan and saline are proved to have the effect of keeping mechanical properties of the cartilage used in moisturizing method.
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In the present study, we investigated the effects of plasma cluster ions on moisturizing skin and increasing comfort sensation in healthy female volunteers. Thirty-two healthy women who often experienced dry skin participated in a randomized crossover trial. We evaluated skin moisture using the Corneometer CM825 and comfort sensation using the visual analogue scale. Plasma cluster ion concentrations of 25000 pcs/cm<sup>3</sup> increased skin moisture, while concentrations of 7000 pcs/cm<sup>3</sup> increased comfort sensation. These results suggest that plasma cluster ions have an effect on skin moisture contents and comfort sensation.<br>
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A hidratação cutânea é, sem dúvida alguma, uma das modalidades terapêuticas mais empregadas pelos dermatologistas em sua prática clínica diária. Neste artigo se apresenta o resultado clínico de um estudo fase IV, aberto, multicêntrico, prospectivo, de uso de extratos de Imperata cylindrica e de Triticum vulgare ceramidas vitaminas A, C, E e F silanol (Epidrat® Ultra) em 168 voluntários portadores de xerose cutânea associada a causas específicas (dermatite atópica, psoríase, xerose senil, hanseníase, climatério e senilidade, diabetes mellitus ou hipotireoismo). Após 30 dias de uso do produto houve alívio, médio, de 40,38% dos sinais (p<0,0001) e 37,08% dos sintomas (p<0,0001) de xerose entre os voluntários (redução média da soma dos sinais e sintomas foi de 40,51% p<0,0001). Antes do uso do produto 61,3% dos voluntários possuíam xerose moderada/grave/muito grave, o que involuiu para 23,2% dos mesmos ao final da avaliação (p<0,0001) inversamente, dos 38,7% dos voluntários considerados com xerose ausente/leve no início do estudo, a porcentagem dos mesmos subiu para 76,2% (p<0,0001, para ambos os comportamentos). Segundo os investigadores, o alívio completo/acentuado da xerose notado foi de 57,1% e, para os voluntários, de 60,7% (p<0,0001). Além do mais, o produto apresentou boa tolerabilidade de uso, com incidência reduzida de eventos adversos realmente relacionados ao seu uso (13,8%) que, em última análise, poderia ser da própria evolução clínica adaptativa da terapêutica tópica, talvez, observada com o uso de qualquer produto tópico, cosmecêutico ou farmacológico. Estes dados, portanto, estabelecem Epidrat® Ultra como uma boa opção terapêutica para abordagem de quadros graves de xerose cutânea.
Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Aged, 80 and over , Dermatologic Agents/administration & dosage , Epidermis/pathology , Fluid Therapy , Plant Extracts/therapeutic useABSTRACT
We evaluated the effects of SCP bathing on the body by compared with those of a-EG, one of major component of SCP, bathing and the control bathing with tap water alone. Healthy adults were used as the subjects. The effects of bathing on blood pressure and pulse rate were not significantly different among the three bathings, indicating that effects of SCP and α-EG bathing on the lung and heart were similar to those of an ordinary bathing. The surface skin temperature at the forehead decreased more slowly in the subjects after taking a SCP bathing than the other two, suggesting that SCP has temperature holding effects. Also, the results of deep body temperature suggested that α-EG might be related to the heat absorbing effects of SCP bathing, which were significantly marked than those of the other two bathings. The present results regarding the changes in blood flow and deep body temperature during bathing suggest that SCP might produce an environment that allows more rapid heat transfer from bath water to the body and less releasing it from the body. Therefore, we concluded that some component other than α-ethylglucoside would be involved in the heat transfer and moisturizing during SCP bathing. Measurement of skin water content indicated that a region directly contacting with hot water was moisturized in a short time, but this condition quickly returned to the pre-bath condition. Whereas, for regions not contacting with bath water, the skin was much more moisturized by taking SCP bath than the other two bathings. Therefore, it was concluded that SCP is effective for enhancement of skin moisturizing.
ABSTRACT
Free amino acids in epidermis function as a major component of Natural Moisturizing Factor (NMF), which maintains the optimal level of water in skin even at the low humidity. In fact, the depletion of free amino acids is reported in the epidermis of atopic dermatitis, the skin condition involving dryness. As an effort searching the dietary source for improving the level of water and free amino acid in epidermis, the dietary effects of silk protein, sericin (S) and fibroin (F) on trans epidermal water loss (TEWL), and plasma and epidermal levels of free amino acids were compared in this study. Thirty of male NC/Nga mice, an animal model of atopic dermatitis, were divided into three groups: group CA as an atopic control with control diet, group S: 1% sericin diet and group F: 1% fibroin diet. Ten of male BALB/c mice were served as group C (control group) with control diet. All mice were fed on diet and water ad libitum for 10weeks. Dry skin condition was established in group CA as TEWL was increased (148.7% of group C). In parallel, epidermal level of glutamate, one of major amino acids functioning as NMF, was dramatically decreased and epidermal levels of methionine and alanine were inversely elevated. Dietary supplementation of sericin (group S) reduced TEWL at the similar level with group C and increased epidermal levels of glutamate as well as serine and glycine, the other major amino acids as NMF. Despite a marked decrease of methionine and alanine, the reduction of TEWL and epidermal levels of glutamate, serine and glycine of group F were less than of group S. Furthermore, in contrast to similar levels of other free amino acids in plasma and epidermis of group S and group C, plasma and epidermal levels of other free amino acids, specifically phenylalanine, isoleucine, cysteine and tyrosine in epidermis of group F, were significantly higher than of group C. Together, our data demonstrate that dietary supplementation of sericin is more effective at improving dry skin condition that paralleled with the normalization of free amino acids in plasma and epidermis of NC/Nga mice.
Subject(s)
Animals , Humans , Male , Mice , Alanine , Amino Acids , Cysteine , Dermatitis, Atopic , Diet , Dietary Supplements , Epidermis , Fibroins , Glutamic Acid , Glycine , Humidity , Isoleucine , Methionine , Models, Animal , Phenylalanine , Plasma , Sericins , Serine , Silk , Skin , TyrosineABSTRACT
BACKGROUND: Natural moisturizing factors such as sodium pyrrolidone carboxylic acid and lactic acid may play an important role in increasing the moisture retention of isolated stratum corneum and reducing the incidence of dry and flaky skin in vivo. Although the precise mechanism of surfactant irritancy is not fully understood, it has been suggested that barrier dysfunction of stratum corneum by surfactants results in skin changes such as scaling, erythema, and even fissuring. OBJECTIVE: We evaluated the effect of sodium pyrrolidone carboxylic acid(NaPCA) and lactic acid(LA) with several non-invasive measuring methods in the irritated skin reaction induced by sodium lauryl sulphate (SLS) in normal persons and atopic dermatitis patients. METHODS: After skin irritation for 24 hours with patch test of 1% SLS on five volar sites of right forearm, we applied nothing(A), 3% LA+3% NaPCA(B), 3% LA(C), 3% NaPCA(D), and vehi cle(E) twice a day respectively. Visual score, transepidermal water loss(TEWL), water holding capa city(WHC), and erythema index were measured at 30 min, 24hr, 48hr and 72hr after patch removal. RESULTS: 1. After 72hr, the visual scores of B and C were significantly lower than that of A(control) in atopic dermatitis patients, and that of C in normal persons was significantly lower than that of A, D, and E. 2. TEWL values of B and C in both the normal (after 72hr) and atopic dermatitis group (after 48hr and 72hr) were significantly lower than that of A. 3. WHC values of B, C, D in both the normal and atopic dermatitis group were significantly higher than that of A after 48hr and 72hr. 4. After 72hr, erythema indices by Mexameter of B, C, and D in both the normal and atopic dermatitis group were significantly lower than that of A and values of C were significantly lower than that of E. In the atopic dermatitis group, values of D were also significantly lower than that of E. 5. The mean visual score was significantly correlated with TEWL value and erythema index of Mexameter (r=0.58, r=0.64) and the TEWL value was significantly correlated with erythema index of Mexameter (r=0.64). CONCLUSION: These results suggest that topical application of a moisturizing factor might improve the surfactant-induced disruption of permeability barrier with improvement of the water holding capacity of the stratum corneum.
Subject(s)
Humans , Dermatitis, Atopic , Erythema , Forearm , Incidence , Lactic Acid , Patch Tests , Permeability , Skin , Sodium , Surface-Active AgentsABSTRACT
The flexibility of surface layer of skin is largely dependent upon the layer's water content. This, in turn, depends primarily on the quantity of water soluble hygroscopic and surface active msterials within the straturn corneum, often known as the natural moisturizing fsctors(NMF). It consist of amino acid, lactic acid, pyrrolidone carboxylic acid and inorganic salts etc.. Some of these components are greatly influenced by hydrogen ion content and ionic form of them were found to be highly hygroscopic. In this experiment, sunburn exfoliated human stratum corneum and pig epidermis separated by 2N-NaBr were used as in vitro test. These tissues were so treated to hsve each pH value (3, 5, 7, 9) and their water holding capacity and hygroscopicity were measured by gravimetric method. The results are summarized as follows : 1, Water holding capactiy of sunburn exfoliated human skin showed significant increase at pH 5 (p<0.1), 7 (p<0.01), 9 (p<0.01) compared to pH 3, and increase in pH 7 (p<0.1), 9 (p<0.05) compared to pH 5 in lactic acid. In pig epidermis, there was significant increase at pH 5 (p<0.05), 7 (p<0.01), 9 (p<0.05) cornpared to pH 3 in lactic acid. 2. Hygroscopicity of pig epidermis showed significant increase at pH 5 (p<0.05), 7 (p<0.1), 9 (p<0.05) in lactic acid and at pH 5 Subject(s)
Humans
, Epidermis
, Hydrogen-Ion Concentration
, Lactic Acid
, Pliability
, Protons
, Salts
, Skin
, Sunburn
, Water
, Wettability