ABSTRACT
Objective Sepsis is the common cause of death among patients with severe pneumonia.The peroxisome prolifera tor-activated receptor γ (PPARγ),as a nuclear transcription factor,has an anti-inflammatory action.This study was to investigate the correlation of PPARγgene polymorphisms with the clinical outcome of sepsis in the Chinese Hans of East China.Methods Using modified multiple ligase detection reaction,we genotyped 13 mononucleotide polymorphism loci of PPARγ in 194 patients with pneumonia-induced sepsis.After adjusting for age,sex,smoking,drinking,and APACHE Ⅱ scores,we analyzed the correlation of PPARγ gene polymorphisms with the clinical outcomes of sepsis by unconditioned logistic regression analysis.Results Compared with the CC genotype,rs1801282 GC+GG significantly decreased the risk of severe organ dysfunction of the patients (OR=0.11,95％ CI:0.01-0.95,P=0.027).In comparison with the CC+CG genotype,rs2920502 GG markedly reduced the risk of death from sepsis (OR=0.22,95％ CI:0.04-0.99,P=0.043).Conclusion In the Chinese Hans of East China,PPARγgene polymorphisms may be associated with multiple organ dysfunction and death of the patients with pneumonia-induced sepsis.
ABSTRACT
Objective To investigate the association of genetic polymorphisms in glutathione S-transferases T1 (GSTrl), M1 (GSTM1) and P1 (GSTP1) with aristolochic acid nephropathy (AAN) of Chinese people in Wenzhou of China. Methods Fifty-nine patientswith AAN (AAN group) including 29 male and 30 female as well as 157 healthy ethnically matched controls (control group) including 93 male and 64 female were enrolled in this study. The genotypes of GSTT1, GSTMI and GSTP1 were determined by multiple PCR and confronting two-pair primers PCR (CTPP-PCR). Results The genotype frequencies of GSTP1 were in Hardy-Weinberg equilibrium. Compared with the healthy controls, the frequency of GSTT1 null genotype was significantly higher in the patients with AAN (66.1% vs 48.4%,P＜0.05). Risk of A.AN for individuals with GSTT1 null genotype was 1.747 fold of those without GSTIl null genotype (95% CI=0.818-3.731). The frequency of GSTM1 null genotype, GSTP1 variant genotypes and GSTP1 G allele in the patients and in the controls were 40.7%, 28.8%, 16.1% and 47.8%, 31.8%, 17.5%, respectively, which were not significantly different. No significant differences were found in prevalence of GSTM1 and GSTP1 gene distribution between patients and controls. Conclusion GSTrl gene polymorphism appears to be associated with susceptibility to AAN in Southern China.