Bases informacionales para el estudio morfométrico de placentas en embarazos gemelares

RESUMEN Introducción: En Cuba existen muy pocos estudios morfofuncionales de placentas procedentes de embarazos gemelares que permitan establecer un patrón morfométrico general. Implementar estas bases informacionales contribuiría a perfeccionar las acciones del Programa Nacional de Salud Sexual y Reproductiva en el país. Objetivo: Sistematizar información sobre estudios morfométricos en placentas gemelares para construir la base epistémica de la caracterización del patrón morfométrico de las placentas gemelares y su relación con la corionicidad, la función de este órgano y el grado de bienestar fetal y neonatal. Método: Se aplicó una estrategia de búsqueda en el período enero de 2020 hasta abril de 2021, en bases de datos internacionales (Medline, EMBASE, LILACS, Scopus, Google Académico) para la revisión bibliográfica, mediante la utilización del método de análisis y crítica de las fuentes desde el procedimiento de análisis de contenido. Se empleó un algoritmo para la toma de decisiones en torno a la pertinencia, actualidad novedad y aportes. Resultados: Se hallaron 16 fuentes bibliográficas sobre el tema de investigación, entre ellas: solo una tesis (6,25 %) y 4 textos editados (25 %). Predominaron los artículos científicos (68,75 %) de los últimos cinco años (93,75 %). Todas las fuentes contribuyeron a la construcción de los referentes teóricos. Conclusiones: El estudio morfométrico de la placenta en embarazos gemelares es un área insuficientemente desarrollada en el conocimiento de las ciencias básicas biomédicas, dada la escasa producción bibliográfica sobre el tema.

ABSTRACT Introduction: In Cuba there are very few morphofunctional studies of placentas from twin pregnancies that allow establishing a general morphometric pattern. Implementing these informational databases would help to improve the actions of the National Sexual and Reproductive Health Program in the country. Objective: To systematize information on morphometric studies in twin placentas to build the epistemic basis for the characterization of the morphometric pattern of twin placentas and its relationship with chorionicity, the function of this organ and the degree of fetal and neonatal well-being. Method: A search strategy was applied in the period January 2020 to April 2021, in international databases (Medline, EMBASE, LILACS, Scopus, Google Scholar) for the bibliographic review, using the analysis and criticism method. of the sources from the content analysis procedure. An algorithm was used to make decisions about relevance, news, news and contributions. Results: 16 bibliographic sources were found on the research topic, among them: only one thesis (6.25%) and 4 edited texts (25.0%). Scientific articles (68.75%) from the last five years (93.75%) predominated. All the sources contributed to the construction of the theoretical referents. Conclusions: The morphometric study of the placenta in twin pregnancies is an insufficiently developed area in the knowledge of basic biomedical sciences, given the scarce bibliographic production on the subject.

RESUMO Introdução: Em Cuba existem poucos estudos morfofuncionais de placentas de gestações gemelares que permitam estabelecer um padrão morfométrico geral. A implementação desses bancos de dados informativos ajudaria a melhorar as ações do Programa Nacional de Saúde Sexual e Reprodutiva no país. Objetivo: Sistematizar informações sobre estudos morfométricos em placentas gêmeas para construir a base epistêmica para a caracterização do padrão morfométrico de placentas gêmeas e sua relação com a corionicidade, a função desse órgão e o grau de bem-estar fetal e neonatal. Método: Foi aplicada uma estratégia de busca no período de janeiro de 2020 a abril de 2021, em bases de dados internacionais (Medline, EMBASE, LILACS, Scopus, Google Scholar) para a revisão bibliográfica, utilizando o método de análise e crítica das fontes da análise de conteúdo procedimento. Um algoritmo foi usado para tomar decisões sobre relevância, notícias, notícias e contribuições. Resultados: Foram encontradas 16 fontes bibliográficas sobre o tema da pesquisa, entre elas: apenas uma tese (6,25%) e 4 textos editados (25,0%). Predominaram artigos científicos (68,75%) dos últimos cinco anos (93,75%). Todas as fontes contribuíram para a construção dos referentes teóricos. Conclusões: O estudo morfométrico da placenta em gestações gemelares é uma área pouco desenvolvida no conhecimento das ciências biomédicas básicas, dada a escassa produção bibliográfica sobre o assunto.

Effects of Ginkgo Biloba Extract and Troxerutin on the Hippocampus of Adult Albino Rats after Induction of Diabetes Mellitus

This study aimed to assess the effects of Ginkgo Biloba Extract and Troxerutin on the hippocampus of induced diabetes mellitus in adult albino rats using histological methods.50 adult male albino rats were divided into three groups; Group I (Control); Group II (diabetic): subdivided into Subgroup IIa (T1DM)), Subgroup IIb (T1DM+GBE), Subgroup IIc (T1DM+ troxerutin); Group III: subdivided into Subgroup IIIa (GBE) and Subgroup IIIb (troxerutin). The brain was removed and the cerebral hemisphere was coronally cut at the hippocampal level and used for light microscopic study (H&E staining and PCNA immunostaining). There was a statistically insignificant improvement in animal weights in subgroup IIb and subgroup IIc. Subgroup IIb showed a statistically significant reduction of blood glucose levels while the subgroup IIc showed insignificant reduction of blood glucose levels. Diabetes disturbed the light microscopic structure of the hippocampus. In subgroup IIb and subgroup IIc the hippocampus retained an apparently normal appearance and the stratum pyramidale exhibited the pyramidal cells with rounded vesicular nuclei and acidophilic cytoplasm. Diabetic hippocampal sections revealed negative PCNA immunoreactivity inall layers of DG. In subgroup IIb and subgroup IIc, hippocampal sections showed positive immunoreactivity

Morphometric MRI study of the brain ventricles in healthy Turkish subjects / Estudio morfométrico de resonancia magnética de los ventrículos cerebrales en sujetos turcos sanos

The aim of this study was to determine the normal values of brain ventricles and indices in healthy subjects in our population using magnetic resonance imaging (MRI) and to reveal sex and age related differences. The MRI of two hundred-sixty-five healthy individuals aged between eighteen and eighty-seven years were examined and the midsagittal and axial images were used for measurements. The measurements were performed from MRI on a Workstation. The following mean values of brain ventricles and indices were observed; frontal horn width (FHW) (33.14 mm); third (3rd) ventricle width (TVW) (3.37 mm); fourth ventricle anteroposterior width (FVWAP) (9.93 mm); fourth ventricle transverse width (FVWT) (12.40 mm); and the maximum transverse inner diameter of the skull (TIDS) (128.75 mm) in females. The same dimensions were 34.85 mm, 3.91 mm, 10.26 mm, 12.81 mm, and 134.68 mm in males, respectively. There were statistically significantly differences in the frontal horn width, third (3rd) ventricle width, and the maximum transverse inner diameter of the skull values in between sexes. The mean values of Evans' index which obtanied with maximum width between the frontal horns of the lateral ventricles divided by the maximum transverse inner diameter of the skull were found as 0.280 ±0.172 in females; whereas the same dimensions were calculated 0.276±0.161 in males. These values were lower in healthy male subjects than females, however; there were no found significantly difference between groups. Present findings obtained from MRI are necessary anatomical baseline data for interpreting pathological changes, planning surgery, and determining presence and progress of some neurological diseases.

El objetivo de este estudio fue determinar los valores normales de los ventrículos e índices cerebrales en sujetos sanos en nuestra población mediante el uso de imágenes de resonancia magnética (RM) y revelar las diferencias relacionadas con el sexo y la edad. Se examinó la resonancia magnética de 265 individuos sanos de entre 18 y 87 años, y se utilizaron las imágenes en sentido medio y sagital para las mediciones. Las mediciones se realizaron a partir de IRM en una estación de trabajo. Se observaron los siguientes valores medios de ventrículos e índices cerebrales: longitud del asta frontal (FHW) (33,14 mm); longitud del tercer ventrículo (TVW) (3,37 mm); longitud anteroposterior del cuarto ventrículo (FVWAP) (9,93 mm); longitud transversal del cuarto ventrículo (FVWT) (12,40 mm); y el diámetro transversal máximo del cráneo (SID) (128,75 mm) en las hembras. Las mismas dimensiones fueron 34,85 mm, 3,91 mm, 10,26 mm, 12,81 mm y 134,68 mm en machos, respectivamente. Hubo diferencias estadísticamente significativas en el ancho del asta frontal, el ancho del tercer ventrículo y el diámetro interno transversal máximo de los valores del cráneo entre los sexos. Los valores medios del índice de Evans que obtuvieron el ancho máximo entre los cuernos frontales de los ventrículos laterales dividido por el diámetro interno transversal máximo del cráneo se encontraron en 0,280 ± 0,172 en las mujeres; mientras que las mismas dimensiones se calcularon en hombres (0,276 ± 0,161). Sin embargo, estos valores fueron más bajos en hombres sanos que en mujeres; no se encontraron diferencias significativas entre los grupos. Los hallazgos actuales obtenidos de IRM son datos anatómicos de referencia necesarios para interpretar los cambios patológicos, planificar la cirugía y determinar la presencia y el progreso de algunas enfermedades neurológicas.

A Morphometric Study Of Body And Vertebral Canal Of Thoracic Vertebrae & Its Clinical Significance

Introduction: Accurate anatomical descriptions of the size, shape and orientation of the main structures of thehuman vertebrae and intervertebral discs are necessary for a variety of approaches and objectives such as theidentification of clinical situations that are related to the morphometry of the spine structures, such as theincidence of low-back pain related to the spinal canal size. So the present study was done to measure the variousmorphometric parameters of thoracic vertebral body and neural canal.Materials and Methods: Total 100 sets of dry human thoracic vertebra were obtained. These are of unknown ageand sex. All Morphometric parameters were measured by digital Vernier caliper of accuracy of 0.01mm. Anteriorheight of the body (VBAH), Posterior height of the body (VBPH), Antero-posterior Diameter of Vertebral body(VBAPD), Transverse Diameter of Vertebral body (VBTD), Anteroposterior diameter of Vertebral Canal (VCAPD) andTransverse diameter of Vertebral Canal (VCTD). All parameters were entered into excel sheet and analysis wasdone by SPSS.Results: Mean VBAH ranged from 13.17+1.35mm (T1) to 17.92+2.25mm (T12), mean VBPH from 15.01+1.27mm(T1) to 20.92+4.58mm (T12), mean VBAPD ranged from 11.62+1.96mm (T1) to 18.12+5.71mm (T12), mean VBTDfrom 24.28+5.68mm (T1) to 28.59+5.97mm (T12), mean VCAPD ranged from 12.46+1.30mm (T1) to 16.05+2.41mm(T12) and VCTD from 17.15+2.19mm (T1) to 20.11+3.74mm (T12).Conclusion: The results of the present study may help in designing implants and instrumentations; understandingspine pathologies; and management of spinal disorder

Types and morphometric study of calcaneal articular facets on human tali of Thai population / Tipos y estudio morfométrico de las facetas articulares calcáneas en talus humano de la población tailandesa

Patterns and variant morphometries of calcaneal articular facets on talus are concerned before performing of joint ankle surgery, including used as a sex determination. Types of talar facets have been documented in many populations except in Thai race. Therefore, this study attempted to classify the types of talus facets and to measure the facet lengths on dried tali of Thais. The 372 dried tali (204 males, 168 females) from Khon Kaen University Bone Collection were observed for variant types and measured for their facet lengths. The facets were classified into 6 types: type I, tree facet are separated (1.88 %); type II (A), the anterior and middle facets are partially connected with predominant ridge (34.68 %); type II (B), the anterior and middle facets are partially connected with slight ridge (32.53 %); type III, the anterior and middle facets are fully fused to form a single facet (2.96 %); type IV, the anterior and middle facets are partially separated by a ridge and partly by a groove (27.42 %); type V, all facets are continuous fused to form a single facet (0.54 %). In addition, the morphometric lengths of AP (anterior to posterior process) and ML1&2 (medial to lateral process) in male are significantly greater than those of female. The AP, ML, and ML2 of male are 56.71±0.16, 41.63±0.18, and 37.85±0.36 mm, while of female are 51.21±0.12, 37.74±0.16, and 33.85±0.28 mm, respectively. This incidence can be used as ankle surgery consideration and an anthropological marker for sex determination of unidentified talus.

Los patrones y las variantes morfométricas de las facetas articulares calcáneas en el talus deben considerarse antes de la realización de la cirugía del tobillo a nivel articular, incluido su uso para la determinación del sexo. Se han documentado los tipos de facetas en el talus en muchas poblaciones, excepto en la raza tailandesa. Por lo tanto, este estudio clasificó los tipos de facetas del talus y se midieron las longitudes de las facetas del talus, en muestras secas de indiviuos tailandeses. Se analizaron 372 talus secos (204 de hombres, 168 de mujeres) de la colección de huesos de la Universidad Khon Kaen, se observaron distintos tipos de variantes y se midieron las longitudes de las facetas. Las facetas se clasificaron en 6 tipos: tipo I, faceta de árbol separadas (1,88 %); tipo II (A), facetas anterior y media parcialmente conectadas con la cresta predominante (34,68 %); tipo II (B), facetas anterior y media están parcialmente conectadas con una ligera cresta (32,53 %); tipo III, facetas anterior y media están completamente fusionadas para formar una sola faceta (2,96 %); tipo IV, facetas anterior y media están parcialmente separadas por una cresta y en parte por una ranura (27,42 %); tipo V, todas las facetas se fusionan continuamente para formar una sola faceta (0,54 %). Además, las longitudes morfométricas del proceso anterior o posterior (AP) y del proceso medial a lateral (ML1 y ML2) en el varón son significativamente mayores que las de la mujer. El AP, ML1 y ML2 de los hombres son 56,71 ± 0,16, 41,63 ± 0,18 y 37,85 ± 0,36 mm, mientras que las mujeres son 51,21 ± 0,12, 37,74 ± 0,16 y 33,85 ± 0,28 mm, respectivamente. Esta incidencia se puede utilizar como una consideración en la cirugía de tobillo y un marcador antropológico para determinar el sexo del talus no identificado.

Potential Toxicity of Plant Growth Regulator Gibberellic acid (GA3) on the Pancreatic Structures and Functions in the albino rat.

Background: Gibberellic acid (GA3) affects many mechanisms of plant growth including stem elongation by stimulating rapid cell division and elongation, flowering, fruit development and breaking dormancy. GA3 is highly persistent and bioactive in soil for months. Since it is easily absorbed dermally, orally or by inhalation; it can injure liver, kidney, muscle and brain tissues. Aim of work: to explore the toxic effect induced by ingestion of residues of GA3 on the pancreas. Methods: Sixty male albino rats, were divided into four equal groups: Group I (negative control): received free water. Group II (positive control): received orally 30± 3ml of solution contained 1 N NaOH 5days/week for 6 weeks. Group III (Treated group): received orally daily 2.2± 0.3mg of GA3 5days/week for 6 weeks .Group IV (recovery group): take the same doses and period similar to group III and left without treatment for another 6 weeks. Fasting blood glucose levels were assessed two times per week to all rats during the experiment. At the end of the experimental period, Malondialdehyde (MDA), Superoxide dismutase (SOD) and Glutathione peroxidase (GSHPX) and Catalase (CAT) were determined, in addition to serum amylase and lipase activity. Moreover, histological examination of the pancreatic tissue was carried by light and electron microscopes further-more, insulin immunohistochemical activity and morphometric study were done. Results: Ingestion of residues of GA3 for 6weeks cause significant elevation (P < 0.001) of MDA and significant drop (P < 0.001) of GSH-PX, SOD and CAT. By stoppage of GA3 lipid peroxidation profile didn't improve completely and still increased above the control levels. Also, GSH-PX and SOD and CAT still decreased significantly. Histological examination express cellular damage with degenerative changes in cells of islet of Langerhans in the form of less population of cells which contained vacuolated cytoplasm and deeply stained or pyknotic nuclei with presence of dilatation of the fenestrated capillary. Ultra structural results of the acinar cells showed irregular contours of nuclei, dilated irregular rough endoplasmic reticulum and reduction of the quantity of the secretory granules with presence of multiple cytoplasmic vacuolations in addition to presence of auotophagic vacuoles. Insulin immunohistochemical staining of islets showing small, atrophied andnegative insulin-immunoreactive spots. The morphometric results represent significant reduction (P < 0.001) in the number of islets/pancreas sections and the number of beta cells/islet. The insult which denoted didn't resolve completely by the 6 week recovery period which may suspect the occurrence of chronic pancreatitis due to oxidative stress. Conclusion: Residual doses of GA3 exposed the pancreases to oxidative stress and 6 weeks is not enough to complete full recovery.

A Morphometric Study of the Obturator Nerve around the Obturator Foramen

OBJECTIVE: Obturator neuropathy is a rare condition. Many neurosurgeons are unfamiliar with the obturator nerve anatomy. The purpose of this study was to define obturator nerve landmarks around the obturator foramen. METHODS: Fourteen cadavers were studied bilaterally to measure the distances from the nerve root to relevant anatomical landmarks near the obturator nerve, including the anterior superior iliac spine (ASIS), the pubic tubercle, the inguinal ligament, the femoral artery, and the adductor longus. RESULTS: The obturator nerve exits the obturator foramen and travels infero-medially between the adductors longus and brevis. The median distances from the obturator nerve exit zone (ONEZ) to the ASIS and pubic tubercle were 114 mm and 30 mm, respectively. The median horizontal and vertical distances between the pubic tubercle and the ONEZ were 17 mm and 27 mm, respectively. The shortest median distance from the ONEZ to the inguinal ligament was 19 mm. The median inguinal ligament lengths from the ASIS and the median pubic tubercle to the shortest point were 103 mm and 24 mm, respectively. The median obturator nerve lengths between the ONEZ and the adductor longus and femoral artery were 41 mm and 28 mm, respectively. CONCLUSION: The obturator nerve exits the foramen 17 mm and 27 mm on the horizontal and sagittal planes, respectively, from the pubic tubercle below the pectineus muscle. The shallowest area is approximately one-fifth medially from the inguinal ligament. This study will help improve the accuracy of obturator nerve surgeries to better establish therapeutic plans and decrease complications.

Morphometric evaluation of the uncinate process and its importance in surgical approaches to the cervical spine: a cadaveric study

<p><b>INTRODUCTION</b>The uncinate process (UP) has an important role because of its relationship with the vertebral artery and spinal roots. Degenerative diseases cause osteophyte formation on the UP, leading to radiculopathy, myelopathy and vertebral vascular insufficiency, which may require surgical management. This study aimed to evaluate the morphometry of this region to shed light on the anatomy of the UP.</p><p><b>METHODS</b>Morphometric data was obtained from 13 male formaldehyde-fixed cadavers. Direct measurements were obtained using a metal caliper. Computed tomography (CT) morphometry was performed with the cadavers in the supine position.</p><p><b>RESULTS</b>Direct cadaveric measurements showed that the height of the UP increased from C3 (5.8 ± 1.0 mm) to C7 (6.6 ± 0.5 mm). On CT, the corresponding measurements were 5.9 ± 1.2 mm at C3 and 6.9 ± 0.6 mm at C7. The distance between the left and right apex of the UP from C3 to C7 also increased on both direct cadaveric and CT measurements (C3: 20.8 ± 1.0 mm and C7: 28.1 ± 2.4 mm vs. C3: 23.7 ± 3.4 mm and C7: 29.0 ± 3.0 mm, respectively). On CT, the distance between the UP and superior articular process at the C3 to C7 levels were 9.8 ± 1.7 mm, 7.9 ± 1.8 mm, 7.9 ± 1.6 mm, 7.8 ± 1.3 mm and 8.2 ± 1.7 mm, respectively.</p><p><b>CONCLUSION</b>Direct cadaveric and CT measurements of the UP are useful for preoperative evaluation of the cervical spine and may lead to better surgical outcomes.</p>

Morphometric study of interparietal bone in Gujarat region.

Background: The occipital bone develops partly in cartilage and partly in membrane. The squamous part of occipital bone between two parietal bones occasionally presents a separate bones which are termed as inca, pre-interparietal bone or interparietal bone. Aims & Objective: (1) To determine the incidence of interparietal bone in skulls of Gujarat region. (2) To study different anomalies of interparietal bone and compare it with other studies. Material and Methods: Total 289 dried macerated skulls from Gujarat region were studied for incidence and type of interparietal bone anomaly. Results: Out of total 289 skulls interparietal bone was found in 23 bones giving incidence of 7.96%. Eight different varieties of interparietal bone anomalies were found and noted. Conclusion: Different anomalies of interparietal bone can be easily interpreted using the knowledge of ossification of interparietal part of occipital bone. Pre-interparietal bone is a misnomer and should not be reported separately.

Allometric growth patterns in hatchery-reared larvae of the catfish Lophiosilurus alexandri (Pisces: Pseudopimelodidae) / Padrões de crescimento alométrico em larvas criadas em cativeiro do pacamã Lophiosilurus alexandri (Pisces: Pseudopimelodidae)

The aim of the present study was to characterize the relationships that occur between morphometric variables of larvae of Lophiosilurus alexandri Steindachner, 1876 through their allometric growth analysis. Total length, head length and body height were correlated with standard length, head height and body height, as well as eye diameter and head height with head length. The results revealed allometric positive relation during the initial development, where the b coefficient varied between 1.10 and 2.81. The variable pre-anal distance in relation to the standard length and the snout length in relation to the head length revealed an allometric negative relation, where the b coefficient was 0.85 and 0.94, respectively.

O objetivo do presente estudo foi caracterizar os tipos de relações que ocorrem entre as variáveis morfométricas das larvas de Lophiosilurus alexandri Steindachner, 1876, por meio da análise de seu crescimento alométrico. As variáveis comprimento total, comprimento da cabeça e altura do corpo foram correlacionadas com o comprimento padrão; a altura da cabeça com a altura do corpo; e o diâmetro do olho e a altura da cabeça com o comprimento da cabeça. Os resultados revelaram relações alométricas positivas durante o desenvolvimento inicial, quando o coeficiente b variou entre 1,10 e 2,81. A variável distância pré-anal em relação ao comprimento padrão e a variável comprimento do focinho em relação ao comprimento da cabeça revelaram relação alométrica negativa quando o coeficiente b foi 0,85 e 0,94, respectivamente.

Estudio morfométrico comparativo entre los músculos bíceps braquial y braquial con el objetivo de establecer el principal músculo flexor del codo / Comparative morphometric study among the brachial and brachial biceps muscles with objective of choosing the main muscle of the flexing of the elbow

El presente trabajo se propone comparar los diámetros de los vientres del músculo bíceps braquial y músculo braquial con el objeto de esclarecer algunas dudas sobre cuál sería el músculo más potente en la flexión del antebrazo. Las comparaciones fueron realizadas en 50 miembros superiores de cadáveres fijados. Se hizo evidente la diferencia promedio de 0,51 cm (5,8 por ciento) en el diámetro del vientre muscular del músculo braquial, ratificando lo encontrado en la revisión bibliográfica, donde el músculo braquial es un importante flexor del codo.

The present work seeks to compare the diameter of the wombs of the muscle brachial biceps and m. brachial in the intention of settling the doubts on which would be the most potent muscle in the flexing of the forearm. The measures were accomplished in 50 superior members of fastened corpses. A medium difference of 0.51 cm (5.8 percent) was evident in the diameter of the muscular womb the largest for m. brachial, ratifying what was found in the bibliographical revision, that brachial muscle is an important flexor of the elbow.

Multicentric morphometric study of dry human sacrum of Indian population in Gujarat Region.

Sacrum is a large triangular bone. It is formed by fusion of five sacral vertebrae and forms the caudal region of the vertebral column. It forms posterosuperior wall of the pelvic cavity wedged between the two hip bones. The present study is undertaken to test the validity of the parameters by which it may be possible to know detail morphometry of sacrum and sacral hiatus. The material for the present study consists of 150 adult sacrum of unknown sex. The measuring was done on intact parts of normal bones. Bones showing wear and tear, fracture or any pathology were not considered. Each linear recording was taken to the nearest millimeter. shape and length of the sacral hiatus, level of apex and anteroposterior depth at apex, level of base and transverse width at base were measured with the help of vernier calipers, recorded, tabulated and analyzed. Significant findings in the present study are high, 83 (55.33%) bones showed narrowed sacral canal at the apex (0-3mm), where as previous studies reported 15.6%. This should be kept in mind while applying caudal epidural anaesthesia in Gujarati population.

Estudio morfométrico de mastocitos en tumores cutáneos / Morphometric study of mast cells in cutaneous tumors

Los mastocitos (M) han sido implicados en el crecimiento y diseminación tumoral. Se estudiaron los M en quince casos de cada una de las siguientes patologías: carcinoma basocelular (CBC), carcinoma espinocelular (CEC), melanoma (MM), queratoacantoma (QA) y queratosis seborreica (QS) con tinción de azul de toluidina. Encontramos que los CBC tenían la mayor cantidad de M seguidos en orden por QS, MM, CEC y QA. Se halló una diferencia extremadamente significativa comparando la cantidad de M en CBC con CEC, MM, QA y QS; QS con CEC, MM y QA. La mayor superficie celular de M la tuvo el CEC seguido de QA, MM, CBC y QS. El cotejo de la superficie de los M fue extremadamente significativa entre CBC con CEC, MM, QA; QS con MM, QA; CEC con MM. El CEC también presentó los M con mayor diámetro seguido por QA, MM, QS y CBC. Diferencia extremadamente significativa, de los diámetros de los M, se descubrió entre CBC con CEC, MM, QA; QS con CEC, MM, QA. Los M estaban aumentados en todas las patologías estudiadas comparados con piel normal. La superficie y diámetro mayor de los M en todas las enfermedades respecto de la piel normal fue extremadamente diferente. La superficie y el diámetro de los M fueron inversamente proporcionales a la cantidad de dichas células. No existe correlación entre malignidad y mayor número de mastocitos.

Mast cells (MC) have been implicated in tumor growth and spread. We analyzed MC in fifteen cases of each basal cell carcinoma (BCC), squamous cell carcinoma (SCC), malignant melanoma (MM), keratoacanthoma (KA) and seborrheic keratosis (SK) stained with toluidine blue. We found that BCC had the largest quantity of MC followed by SK, MM, SCC and KA. An extremely significant difference was found comparing MC quantity in BCC vs SCC, MM, KA and SK; SK vs SCC, MM and KA. SCC had the biggest MC area pursued by KA, MM, BCC and SK. The comparisons of MC areas were extremely significant in BCC vs SCC, MM, KA; SK vs MM, KA; SCC vs MM. SCC, also had the longest MC followed by KA, MM, SK and BCC. Extremely significant difference of MC length was found in BCC vs SCC, MM, KA, SK; SK vs SCC, MM, KA. MC were increased in every studied pathology compared with normal skin. The comparisons of MC areas and length of all of these diseases with NS were extremely different. MC area and length were inversely proportional with the quantity of MC. There is no coincidence between malignancy and great amount of MC.

Morphometric Study on the Arterial Palmar Arch of the Hand

PURPOSE: Deviations of arterial palmar arches in the hand can be explained on the embryological basis. The purpose of this study is to provide new information about palmar arches through cadaver's dissection. The values of the location and diameter in these vessels were analyzed in order to support anatomical research and clinical correlation in the hand. METHODS: The present report is based on an analysis of dissections of fifty-three hands carried out in the laboratory of gross anatomy. A reference line was established on the distal wrist crease to serve as the X coordinate and a perpendicular line drawn through the midpoint between middle and ring fingers, which served as the Y coordinate. The coordinates of the x and y values were measured by a digimatic caliper, and statistically analyzed with Student's test. RESULTS: Complete superficial palmar archs were seen in 96.2 % of specimens. In the most common type of males, the superficial arch was formed only by the ulnar artery. In the most common type of females, the superficial arch was formed anastomosis between the radial artery and the ulnar artery. The average length of the superficial and deep palmar arch is 110.3 +/- 33.0 mm and 67.9 +/- 14.0 mm respectively. Regarding the superficial palmar arch, ulnar artery starts -16.1 +/- 5.1 mm on X-line, and 2.5 +/- 24.5 mm on Y-line. Radial artery appears on palmar side 7.7 +/- 3.2 mm on X-line, and 20.9 +/- 10.9 mm on Y-line. But radial artery starts on 6.3 +/- 3.6 mm on X-line, and 3.4 +/- 5.1 mm on Y-line. Digital arteries of superficial palmar arch starts on 6.1 +/- 3.7 mm, 33.9 +/- 8.8 mm on index finger, 1.8 +/- 3.4 mm, 40.1 +/- 7.3 mm on middle finger, -3.2 +/- 4.9 mm, 42.6 +/- 7.0 mm on ring finger, and -8.9 +/- 5.1 mm, 42.5 +/- 80 mm on little finger in respective X and Y coordinates. Radial artery of deep palmar arches measured at the palmar side perforating from the dorsum of hand. It's coordinates were 9.7 +/- 4.8 mm on X-line, 21.7 +/- 10.2 mm on Y-line. Ulnar artery was measured at hypothenar area, and it's coordinates were -20.4 +/- 6.3 mm on X-line, and 30.6 +/- 7.4 mm on Y-line. CONCLUSIONS: Anatomically superficial palmar arch can be divided into a complete and an incomplete type. Each of them can be subdivided into 4 types. The deep palmar arch is less variable than the superficial palmar arch. We believe these values of the study will be used for the vascular surgery of the hand using the endoscope and robot in the future.

The Significance of Nuclear Size in Nuclear Grade of Invasive Ductal Carcinoma of the Breast / 대한세포병리학회지

To make the objective standard of nuclear size in grading nuclear pleomorphism of invasive ductal carcinoma of the breast, we measured maximal nuclear diameter of tumor cells on imprint cytology slides and histologic sections from 65 cases by using computer-based image analysis system(Optimas 6.0). The maximal diameter of red blood cells were also measured to evaluate the ratio of maximal nuclear diameter of tumor cells to maximal diameter of red blood cells. The mean values of maximal nuclear diameter of tumor cells on imprint cytology slides and histologic sections were 7.56 micrometer, 7.53 micrometer in nuclear grade 1, 8.92+/-0.98 micrometer, 9.02+/-0.74 micrometer in nuclear grade 2, and 12.90+/-1.47 micrometer, 12.44+/-1.41 micrometer in nuclear grade 3, respectively. There were no significant differences between values of imprint cytology and histologic section. The ratio of maximal nuclear diameter of tumor cells to maximal diameter of red blood cells were 1.3-1.4:1 in nuclear grade 1, 1.6-1.7:1 in nuclear grade 2, and 2.2-2.3:1 in nuclear grade 3. These values would be guidelines for grading nuclear pleomorphism of invasive ductal carcinoma of the breast on routine surgical pathology work.

Morphometric Study for Muscular and Microvascular Remodeling of Left Ventricular Free Wall and Interventricular Septum in Hypertrophic Cardiomyopathy

Hypertrophic cardiomyopathy (HCMP) is characterized myofiber hypertrophy and structural remodeling with changes in the proportion of the muscular, vascular, and interstitial compartments. A study was done to determine the structural remodeling patterns and the role of microvasculature of hearts in HCMP. Forty-two postmortem hearts were analyzed including 14 from patients who died of HCMP (group 1), 8 patients with HCMP but who died from extracardiac causes (group 2), 10 patients with ischemic heart disease (group 3), and 10 normal adult hearts (group 4). Macro- and microscopic examination, immunohistochemical study using CD34 antibody, and morphometric studies using image analyzer were performed. Mean cardiac weight and wall thickness were significantly higher in the HCMP group. Myocardial hypertrophy, and a variety of myocardial disarray and fibrosis involved the whole area of the left ventricles with HCMP. The percentage areas of microvessels were 6.40 0.7 in group 1, 5.90 0.6 in group 2, 4.98 0.3 in group 3, 4.85 0.4 in group 4, respectively, and the numbers of microvessels were 198.0 20.7 in group 1, 230.0 22.3 in group 2, 211.7 11.2 in group 3, and 236.4 11.4 in group 4, respectively (mean SE). The percentage area of microvessels was significantly higher in group 1 than in other groups. However, the number of microvessels in that group was lower than in the other groups, although it was statistically insignificant. Since flow-dependent vasodilation is preserved in HCMP, we considered flow-dependent vasodilation the cause of the discrepancy between the area and the number of microvessels. Ischemic changes observed in chronic HCMP and related heart failure were considesed to be due to the relative deficiency of the coronary flow compared to the increasing cardiac mass.

Morphometric Study on Fine Needle Aspirates from Follicular Adenoma and Follicular Carcinoma of the Thyroid / 대한세포병리학회지

Fine needle aspiration cytology of "cold" nodules of the thyroid has proved to be of great value in their preoperative diagnosis. Most types of thyroid tumors are readily recognizable from characteristic cellular patterns in the smears of needle aspirates. But follicular neoplasms present some problems because the cytomorphology of the adenomas frequently is same as in carcinoma. For differentiation of benign from malignant follicular neoplasms of the thyroid we tested the usefulness of two objective parameters - nuclear area and perimeter - by morphometry. This study was made on fine needle aspirates from 30 cases with cytologic diagnosis of follicular neoplasm of thyroid. The histologic classification was follicular adenoma in 22 cases and follicular carcinoma in 8 cases. As a reference group we used seven caes with nodular hyperplasia. The smears of aspirates were stained by Papanicolaou method. On each slide 200 randomly selected cells with intact nuclei were measured. The mean value of nuclear area are 25.32+/-5.50 micrometer2, 34.08+/-7.50 micrometer2 and 39.97+/-6.63 micrometer2 in nodular hyperplasia, follicular adenoma, and follicular carcinoma, respectively. The mean value of perimeter are 19.48+/-2.26 micrometer, 22.95+/-2.65 micrometer and 24.78+/-2.23 micrometer in nodular hyperplasia, follicular adenoma and follicular carcinoma, respectively. The mean nuclear areas and perimeters of cells from follicular adenoma were significantly larger than those from nodular hyperplasia (p<0.05). The mean nuclear areas and perimeters of cells from follicular carcinoma were larger than those from follicular adenoma but the differences are not significant statistically(p<0.05). Therefore, morphometric assessment alone is inadequate to predict malignancy in thyroid aspirates.

Structural-Functional Relationships in Renal Amyloidosis

The pathogenetic mechanism of renal dysfunction in renal amyloidosis is poorly understood. To evaluate the morphologic parameters which are correlated with renal function in this disorder, we have examined renal biopsies from 14 patients with renal amyloidosis by morphometry. Of the 14 patients, 8 were male and 6 were female. They were between 41 and 70 years of age. The serum concentration of albumin and creatinine were 2.1+/-0.7 mg/dl and 1.1+/-0.5 mg/dl, respectively. The 24-hour excretion of urinary protein was 7.9+/-5.2 g. Creatinine clearance was 62+/-23 ml/min/1.73m2. The mean glomerular volume (MGV) was (2.2+/-1.3) 10(6) micrometer3. The surface density of peripheral glomerular basement membrane [Sv (PGBM/glom)] was 0.049+/-0.027 (micrometer3/micrometer3). Volume density of mesangium [Vv (mes/glom)] was 0.31+/-0.14 (micrometer3/micrometer3) and volume density of glomerular amyloid deposition [Vv (amyl/glom)] was 0.21+/-0.14 (micrometer3/micrometer3). The volume density of cortical interstitium [Vv (int/cortex)] was 0.14+/-0.09 (micrometer3/micrometer3). The serum creatinine concentration was significantly correlated with Vv (int/cortex) (r=+0.66, p<0.05). MGV was correlated with Vv (mes/glom) (r=+0.75, p<0.01) and Vv (amyl/glom) (r= +0.68, p<0.05) but showed negative correlation with Sv (PGBM/glom) (r=-0.79, p<0.01). Sv (PGBM/glom) showed negative correlation with Vv (mes/glom) (r=-0.77, p<0.01) and with Vv (amyl/glom) (r=-0.87, p<0.01). Positive correlation was observed between Vv (mes/glom) and Vv (amyl/glom) (r=+0.95, p<0.01). These results suggest that the decreased renal function in patients with amyloidosis is related to interstitial fibrosis rather than glomerular lesions. In addition, glomerular hypertrophy in these patients is related to amyloid deposition in the mesangium and peripheral glomerular basement membrane.

IV-gamma Globulin Therapy for Severe Abdomlnal Pain Refractory to Steroid Therapy in Henoch-Scholein Purpura

To make the objective standard of nuclear size in grading nuclear pleomorphism of invasive ductal carcinoma of the breast, we measured maximal nuclear diameter of tumor cells on imprint cytology slides and histologic sections from 65 cases by using computer-based image analysis system (Optimas 6.0). The maximal diameter of red blood cells were also measured to evaluate the ratio of maximal nuclear diameter of tumor cells to maximal diameter of red blood cells. The mean values of maximal nuclear diameter of tumor cells on imprint cytology slides and histologic sections were 7.56microgram, 7.53microgram in nuclear grade 1, 8.92+/-0.98microgram, 9.02+/-0.74microgram in nuclear grade 2, and 12.90+/-1.47prn, 12.44+/-1.41microgram in nuclear grade 3, respectively. There were no significant differences between values of imprint cytology and histologic section. The ratio of maximal nuclear diameter of tumor cells to maximal diameter of red blood cells were 1.3-1.4:1 in nuclear grade 1, 1.6-1.7:1 in nuclear grade 2, and 2.2-2.3:1 in nuclear grade 3. Ths would be guidelines for grading nuclear pleomorphism of invasive ductal carcinoma of the breast on routine surgical pathology work.

Morphometric study of megaureter / 대한비뇨기과학회지

There are different patterns of muscular response according to the type of megaureter. These patterns can be used in determining the cause of the wide ureters. We studied 20 dilated ureters quantitatively and quantitatively by standard pathologic techniques using the average size and population of muscle cells in the dilated ureter as the quantitative index of workload or obstruction. In the obstructed type. the smooth muscle in the wall of the proximal dilated ureter was hypertrophic and hyperplastic. In contrast. in the refluxing type. there was neither hypertrophy nor hyperplasic of muscle in the wall of the proximal dilated ureter. By this morphometric study of muscle cells, it can be possible to identify the megaureters which are caused by obstruction not only in the excised specimen but also by a frozen section biopsy specimen.