ABSTRACT
Objective: To establish the rapid method of multi-component library preparation of large ethnodrug compound prescription and offer large amounts of samples for active components high-throughput screening. Methods: The ethyl acetate extract from Mongolian medicine BaiMai Powder was separated into a series of fractions by linear gradient elution with flash chromatography. The separation efficiency was evaluated by high-performance liquid chromatography. Results: Twenty-nine sequential components were prepared from the ethyl acetate extract of the prescription, enrichment and separation of compounds with different nature were well complished. Conclusion: Multi-component library of ethnodrug compound prescription can be constructed by flash chromatography. This method will help to overcome the difficulty of the pharmacodynamic chemical substances research of large ethnodrug compound prescription.