Gain deeper insights into traditional Chinese medicines using multidimensional chromatography combined with chemometric approaches / 中草药·英文版

Traditional Chinese medicines (TCMs) possess a rich historical background, unique theoretical framework, remarkable therapeutic efficacy, and abundant resources. However, the modernization and internationalization of TCMs have faced significant obstacles due to their diverse ingredients and unknown mechanisms. To gain deeper insights into the phytochemicals and ensure the quality control of TCMs, there is an urgent need to enhance analytical techniques. Currently, two-dimensional (2D) chromatography, which incorporates two independent separation mechanisms, demonstrates superior separation capabilities compared to the traditional one-dimensional (1D) separation system when analyzing TCMs samples. Over the past decade, new techniques have been continuously developed to gain actionable insights from complex samples. This review presents the recent advancements in the application of multidimensional chromatography for the quality evaluation of TCMs, encompassing 2D-gas chromatography (GC), 2D-liquid chromatography (LC), as well as emerging three-dimensional (3D)-GC, 3D-LC, and their associated data-processing approaches. These studies highlight the promising potential of multidimensional chromatographic separation for future phytochemical analysis. Nevertheless, the increased separation capability has resulted in higher-order data sets and greater demands for data-processing tools. Considering that multidimensional chromatography is still a relatively nascent research field, further hardware enhancements and the implementation of chemometric methods are necessary to foster its robust development.

Attitudes towards childcare practices in rural and urban caregivers with high rates of multidimensional poverty

Objective: To analyse the relationship between poverty and attitudes towards childcare practices in rural and urban areas of Colombia. Method: This article presents a cross-sectional correlational study conducted in the department of Atlántico, northern Colombia. Attitudes towards childcare and living conditions were assessed among 1189 caregivers of preschool children. The multidimensional poverty index and the participants' area of origin (urban or rural) were considered. Results: No direct relationship was found between attitudes towards childcare and the multidimensional poverty index. However, it was observed that urban participants from deprived areas had a higher poverty index and less positive attitudes towards childcare, especially regarding cognitive, sleep and health care. There was a moderating effect of area on attitudes towards childcare. Conclusions: The study highlights the influence of poverty and social vulnerability on attitudes towards childcare, particularly in urban areas. These findings underline the importance of considering socio-economic and geographical conditions when addressing childcare practices. It also highlights the need to implement specific strategies to promote positive childcare practices in more vulnerable contexts to improve early childhood development in Colombia.

Objetivo: Analizar la relación entre la pobreza y las actitudes hacia las prácticas de cuidado infantil en áreas rurales y urbanas de Colombia. Método: El presente artículo es un estudio transversal correlacional en el departamento del Atlántico, al norte de Colombia. Se indagó sobre las actitudes hacia el cuidado infantil y las condiciones de vida en 1189 cuidadores de niños en edad preescolar. Se tuvo en cuenta el índice de pobreza multidimensional y el área de procedencia de los participantes (urbana o rural). Resultados: No se encontró una relación directa entre las actitudes hacia el cuidado infantil y el índice de pobreza multidimensional. Sin embargo, se observó que los participantes de áreas urbanas provenientes de zonas vulnerables presentaron un mayor índice de pobreza y mostraron actitudes menos positivas hacia el cuidado infantil, especialmente en el cuidado cognitivo, del sueño y de la salud. Se evidenció un efecto moderador del área sobre las actitudes de cuidado. Conclusiones: El estudio destaca la influencia de la pobreza y la vulnerabilidad social en las actitudes de cuidado infantil, especialmente en áreas urbanas. Estos hallazgos subrayan la importancia de considerar las condiciones socioeconómicas y geográficas al abordar el cuidado infantil. Además, se resalta la necesidad de implementar estrategias específicas para promover prácticas de cuidado positivas en contextos más vulnerables, con el fin de mejorar el desarrollo de la primera infancia en Colombia.

Application and practice of multidimensional teaching method in "Cell Engineering" / 生物工程学报

In order to improve the teaching quality of engineering courses, we introduced a multi-dimensional teaching method into the teaching reform of biology majors in colleges based on the portfolio assessment in the curriculum of Cell Engineering. We reformed the knowledge system, teaching form and implementation scheme of this course. By combining the reform of online teaching, interactive teaching, case teaching and other teaching modes, the students mastered the relevant professional knowledge and the scientific and technological frontier of Cell Engineering. Moreover, their learning interest and enthusiasm, ability of analyzing and solving professional problems related to Cell Engineering also improved. The implementation of teaching reform of this course provides a reference for other similar professional courses in colleges.

Research Ideas and Methods for Standardization of Traditional Chinese Medicine Syndrome Differentiation / 中医杂志

Syndrome differentiation and treatment is a unique mode of diagnosis in traditional Chinese medicine （TCM）. The establishment of scientific and standardized syndrome diagnosis standards is an important link to evaluate the clinical efficacy of TCM objectively and systematically， and also a prerequisite for the promotion and development of TCM to obtain international recognition. This article reviewed the basic modes and existed problems of the current syndrome diagnosis criteria， and proposed to construct a multidimensional core information set integrating the minimized core symptoms， the artificial intelligence signs， the multi-modal laboratory indicators， and multi-omics specific markers， so as to present syndrome characteristics from multiple perspectives systematically. This paper also described the basic mode， constructure， as well as the process and methodology to be adopted in the establishment of the standardized diagnostic research method. The core information set of diagnostic symptoms not only took into account the specificity of the disease， but also improved the inconsistency due to the complexity and subjectivity of the syndrome differentiation， thereby providing a methodological basis for the standardization of TCM syndrome differentiation in clinical research.

Dental non-metric traits of Han and Zhuang people / 解剖学报

[Abstract] Objective To investigate the relationship between the Han, Zhuang and the World populations by comparing the frequency of dental non-metric traits. Methods From 2015 to 2019, ten dental non-metric traits were sampled from He’nan, Jiangsu and Guangxi Zhuang Autonmous Region, with sample size of 1785(674 He’ nan, 547 Jiangsu, 388 Guangxi Han, 176 Guangxi Zhuang). Principal component analysis, multidimensional scaling and neighbor network were carried out. Results East Asian populations could be clearly divided into two major groups, Northeast Asian and Southeast Asian. Han and Zhuang populations were close to the Northeast Asian populations, but were far from the Southeast Asian populations. There were also some north-south differences within Han populations. Conclusion Both Sundadonty and Sinodonty are existed in East Asian populations. Han and Zhuang populations dental non-metric traits are closer to the Sinodonty.

Carbohydrate-based quality consistency evaluation of Saposhnikoviae Radix / 中国中药杂志

This study aims to evaluate the quality consistency of Saposhnikoviae Radix based on carbohydrates, and explore the potential of carbohydrates as the internal quality control indicators of Saposhnikoviae Radix. The total polysaccharides were quantified by UV-Vis spectrophotometry and the molecular weight range of the polysaccharides was determined by high performance gel-permeation chromatography-evaporative light scattering detection(HPGPC-ELSD). The monosaccharides in polysaccharides and the free monosaccharides were quantified by high performance liquid chromatography-UV detection(HPLC-UV), and the oligosaccharides and fructose were quantified by high performance liquid chromatography-evaporative light scattering detection(HPLC-ELSD). The carbohydrate-based quality of Saposhnikoviae Radix was compared among 45 batches of commercial samples and 13 batches of self-collected samples. The results showed that the molecular weight distribution, monosaccharide composition, oligosaccharide, and free monosaccharide composition were similar in the 58 batches of samples. The average content of total polysaccharides, oligosaccharides, and total free monosaccharides in commercial samples were 39.66, 148.79, and 68.62 mg·g~(-1), respectively. The content showed significant differences among batches, with the highest differences of 3.51, 1.75, and 2.58 times, respectively. The RSD of the relative ratios of monosaccharides in the polysaccharides in commercial samples reached 28%-45%. The average content of total polysaccharides, oligosaccharides, and total free monosaccharides in self-collected samples were 68.07, 145.76, and 42.04 mg·g~(-1), respectively, with the inter-region differences of 2.88, 1.88, and 1.07 times, respectively. The RSD of the relative ratios of monosaccharides in polysaccharides in self-collected samples ranged from 8.2% to 59%. The total polysaccharides and total free monosaccharides in self-collected samples were 1.72 times higher and 1.63 times lower, respectively, than those in commercial samples. The content of oligosaccharides was similar between self-collected samples and commercial samples. To sum up, carbohydrates are one of the material bases for the internal quality consistency of Saposhnikoviae Radix. The qualitative characteristics of polysaccharides and the quantitative characteristics of polysaccharides and oligosaccharides are related to the origin of medicinal materials. Moreover, the quantitative characteristics of polysaccharides and free monosaccharides may be related to the storage conditions. Carbohydrates are potential indicators for the quality control of Saposhnikoviae Radix and deserve attention.

Psychometric Properties of the Hewitt and Flett Multidimensional Perfectionism Scale in Brazilian Adults / Propriedades Psicométricas da Escala Multidimensional de Perfeccionismo de Hewitt e Flett para Adultos Brasileiros

Abstract The aim of the present study was to investigate validity evidence based on internal structure and the relationship with other variables of the Brazilian version of the Hewitt and Flett Multidimensional Perfectionism Scale (H&F-MPS). To this end, a sample comprising 368 participants with a mean age of 31.17 years (SD=11,34), 74% female, answered the H&F-MPS perfectionism scale and instruments that measure personality and psychological inflexibility. Findings indicated a factor structure of three dimensions, like the original scale, good discriminative item parameters, highly satisfactory reliability indexes, and significant correlations of the hypothesized directions with Openness to Experiences, Neuroticism, and Conscientiousness factors from the personality measure, as well as with the psychological flexibility measure.

Resumo O objetivo do presente estudo foi levantar evidências de validade da estrutura interna e da relação com outras variáveis para a versão brasileira da Escala Multidimensional de Perfeccionismo de Hewitt e Flett (H&F-MPS). Para isso, uma amostra composta por 368 participantes com média de idade de 31,17 anos (DP = 11,34), sendo 74% do sexo feminino, respondeu à escala de perfeccionismo H&F-MPS e a instrumentos que mensuram a personalidade e a inflexibilidade psicológica. Os resultados apontaram uma estrutura fatorial com três dimensões semelhante à escala original, bons parâmetros de discriminação dos itens, índices muito satisfatórios de confiabilidade e correlações significativas na direção esperada com os fatores abertura à experiência, neuroticismo e conscienciosidade da medida de personalidade e com o instrumento de inflexibilidade psicológica.

Desigualdades sociales en la mortalidad maternal, República Dominicana, 2016 / Social inequalities in maternal mortality, Dominican Republic, 2016

Esta investigación pretende cuantificar las diferencias en la razón de mortalidad materna en las provincias de la República Dominicana, considerando las distintas condiciones sociales a la que estas mujeres están expuestas. Se utilizó la información proveniente de registro de estadísticas vitales. Se calculó la asociación entre la razón de mortalidad materna y ciertos indicadores socioeconómicos seleccionados. Se calcularon las métricas de desigualdad tomando en cuenta los indicadores socioeconómicos que se hallaron significativamente asociados con la razón de mortalidad materna, resultando esta dos veces mayor en los territorios más desfavorecidos socialmente, comparados con los territorios que se encontraron en mejores condiciones sociales.

This research aims to quantify the differences in the ma-ternal mortality rate in the provinces of the Dominican Republic, considering the different social conditions to which these women are exposed. Information from the vital statistics registry was used. The association be-tween the maternal mortality rate and certain selected socioeconomic indicators was calculated. The inequality metrics were calculated considering the socioeconomic indicators that were found to be significantly associated with the maternal mortality rate, resulting in two times higher in the most socially disadvantaged territories, compared to the territories that were found in better social conditions.

Social Isolation, Social Support, and Psychological Distress Among the Elderly During the COVID-19 Pandemic: A Cross-Sectional Study from Central India

Background: In the present COVID-19 pandemic, social distancing measures have been advised to protect elderly from infection which might have led to poor mental health state. Objective: A cross-sectional study was carried out to assess the magnitude of social isolation, social support, and psychological distress among the elderly during the COVID-19 pandemic in Central India. Methods: The estimated sample size was 1535. The sample was equally distributed among rural, semiurban, and urban strata of districts. Social isolation was measured using Lubben’s Social Network Scale?Revised, and psychological distress was assessed using Kessler K10 Psychological Distress Scale. Other parameters such as a history of COVID-19 illness and COVID-19 vaccination were assessed. Results: The prevalence of social isolation was higher at 23.6% during the COVID?19 pandemic compared to before the pandemic period (15.0%). The majority perceived a high level of social support during the pandemic (55.3%) and 39.9% received moderate support. Overall, 18.4% of the respondents had psychological distress. Out of them, 56.2% had mild distress, 20.1% had moderate distress, and 23.7% had severe distress. Significant predictors of psychological distress were female gender, lower socioeconomic status, history of COVID-19 disease among the participants, social isolation, and lack of social support. Conclusion: Social isolation and lack of social support were significant predictors of psychological distress among the elderly during the pandemic.

Evidências de validade da Escala Multidimensional de Suporte Social Percebido (EMSSP) em universitários / Evidence of the validity of the Multidimensional Scale of Perceived Social Support (MSPSS) in university students

Resumo O apoio social se refere aos recursos sociais percebidos como disponíveis à pessoa e tem sido fortemente associado a desfechos em saúde física e mental. O estudo atual objetivou avaliar a consistência interna, a validação de construto e a confiabilidade teste-reteste da Escala Multidimensional de Suporte Social Percebido (EMSSP) em universitários do Centro Oeste brasileiro. As análises fatoriais exploratória e confirmatória foram realizadas com duas amostras aleatórias e independentes, ambas de 1.147 universitários da área da saúde. O estudo teste-reteste foi realizado com uma amostra de 347 estudantes de medicina e duas coletas com intervalo de 14 dias entre elas. A melhor solução foi com três fatores: família, amigos, e outros significativos, os quais apreesentaram cargas fatoriais variando entre 0,767 a 0,950 e consistência interna elevada (alfa de Cronbach de 0,96, 0,97 e 0,97, respectivamente). O ajustamento para essa solução foi adequado (χ2(g.l.)=380 (49), p<0,001; SRMR: 0,023; RMSEA: 0,077; CFI: 0,993; TLI: 0,990). A confiabilidade teste-reteste foi moderada (Kappa ponderado variando de 0,36 a 0,52). Os resultados sugerem que a EMSSP possui propriedades psicométricas aceitáveis para seu uso com universitários.

Abstract Social support refers to the social resources that a person perceives to be available and has been strongly associated with physical and mental health outcomes. The present study aimed to evaluate the internal consistency, construct validity, and test-retest reliability of the Perceived Social Support Multidimensional Scale (PSSMS) in university students in the Midwest of Brazil. Exploratory and confirmatory factor analyses were performed with two independent random samples, both consisting of 1,147 students. The test-retest study was carried out with a sample of 347 medical students. Data were collected in two separate moments with a 14-day interval. The solution with the best fit was the three-factor model: family, friends, and significant others. The factor loadings of the scale items ranged between 0.767 and 0.950 and each factor showed high internal consistency (Cronbach's alpha=0.96, 0.97, and 0.97, respectively). The model also attained adequate fit: χ2(d.f.)=380 (49), p<0.0001; SRMR=0.023; RMSEA=0.077; CFI=0.993; TLI=0.990. Test-retest reliability was moderate (weighted kappa ranging from 0.36 to 0.52). The results suggest that the PSSMS has acceptable psychometric properties for use with university students.

Evidencia de validez de la Escala Multidimensional de Apoyo Social Percibido (EMASP) en población adulta Argentina / Evidence of Validity of the Multidimensional Social Support Scale (MSPSS) in adult Argentinian population

Resumen El presente trabajo tuvo como objetivo adaptar y validar la Escala Multidimensional de Apoyo Social Percibido (EMASP) para adultos argentinos. La EMASP cuenta con 12 items que permiten indagar acerca del apoyo social en tres fuentes: Familia, Amigos y Otros significativos. Para este trabajo, se ha escogido un diseño instrumental. La muestra fue de tipo intencional, compuesta por 1278 adultos nacidos en Argentina con edades que oscilan entre los 18 y 83 años y de ambos sexos. En el contexto local, la EMASP muestra propiedades psicométricas adecuadas para cada uno de los factores. La validez cruzada por sexo indica que la Escala presenta índices de ajuste adecuados tanto para hombres como para mujeres. El análisis de la validez externa dio cuenta de las relaciones indirectas entre las distintas dimensiones de la ESMAP con el cuestionario Patient Health Questionnaire (PQH9), que evalúa trastorno depresivo mayor. Se concluye que la EMASP presenta propiedades psicométricas adecuadas para su uso en población argentina.

Abstract The present work aimed to adapt and validate the Multidimensional Scale of Perceived Social Support (EMASP) for Argentine adults. The EMASP has 12 items that allow inquiring about social support from three sources: Family, Friends and Significant others. For this work, an instrumental design has been chosen. The sample was of an intentional type, consisted of 1278 adults born in Argentina aged between 18 and 83 of both sexes. In the local context, the EMASP shows adequate psychometric properties for each of the factors. Cross-validity by sex indicates that the Scale has adequate adjustment indices for both men and women. The external validity analysis revealed the indirect relationships between the different dimensions of the ESMAP with the Patient Health Questionnaire (PQH9) which assesses major depressive disorder. It is concluded that the EMASP has adequate psychometric properties for its use in the Argentine population.

Application of SifaInDel 45plex System in the Han and Mongolian Populations / 法医学杂志

OBJECTIVES@#To investigate the genetic polymorphism of InDel loci in SifalnDel 45plex system in the Han population in Jiangsu Province and the Mongolian population in Inner Mongolia, and to evaluate the effectiveness of the system in forensic medicine.@*METHODS@#SifaInDel 45plex system was used for genotyping in blood samples of 398 unrelated individuals from the above two populations, and allele frequencies and population genetic parameters of the two populations were calculated respectively. Eight intercontinental populations in the gnomAD database were used as reference populations. The genetic distances between the two studied populations and eight reference populations were calculated based on the allele frequencies of 27 autosomal-InDels (A-InDels). The phylogenetic trees and multidimensional scaling (MDS) analysis diagrams were constructed accordingly.@*RESULTS@#Among two studied populations, the 27 A-InDels and 16 X-InDels showed no linkage disequilibrium between each other and the allele frequency distributions were in Hardy-Weinberg equilibrium. The CDP of the 27 A-InDels in two studied populations were all higher than 0.999 999 999 9, and the CPEtrio were all less than 0.999 9. The CDP of the 16 X-InDels in Han in Jiangsu and Mongolian in Inner Mongolia female and male samples were 0.999 997 962, 0.999 998 389, and 0.999 818 940, 0.999 856 063, respectively. The CMECtrio were all less than 0.999 9. The results of population genetics showed that the Jiangsu Han nationality, Inner Mongolia Mongolian nationality and East Asian population clustered into one branch, showing closer genetic relationship. The other 7 intercontinental populations clustered into another group. And the above 3 populations displayed distant genetic relationships with the other 7 intercontinental populations.@*CONCLUSIONS@#The InDels in the SifaInDel 45plex system have good genetic polymorphism in the two studied populations, which can be used for forensic individual identification or as an effective complement for paternity identification, and to distinguish different intercontinental populations.

Study on influencing factors and needs of medical personnel in scientific research of a tertiary hospital in Jieyang / 中华医学科研管理杂志

Objective:To understand the needs, problems and challenges of medical personnel in the hospital for scientific research, propose tailored management measurements and suggestions for the hospital.Methods:The questionnaire was prepared by " Questionnaire Star" , the convenience sampling method was used, and information was collected by WeChat online chat group. Factor analysis and multi-dimensional preference analysis were adopted to analyze information collected.Results:Four main factors that affect the scientific research of medical personnel were identified. The most significant impact is the lack of information platform, followed by the lack of environmental atmosphere, lack of personal capacity and lack of personal interest. The research needs of medical personnel are divided into two categories: one is the more basic skill needs, which are mainly targeted at female, nurses, undergraduates, 5~10 years′ working experiences, medium-grade professional title, and people with no knowledge and experience in research. The other one is the needs of skill improvement, which are mainly targeted at younger, physicians, early career, lower professional titles, and had certain research knowledge.Conclusions:So far, there is a lot of space for improvement. It is recommended to promote the capacity building by training, strengthen the research team building and construct of research communication platform, at the same time, update the scientific research management system.

The reliability and validity of multi-dimensional, quantitative video evaluation of the hand function of stroke survivors / 中华物理医学与康复杂志

Objective:To test the reliability and validity of a multi-dimensional, quantitative video evaluation system (the Multi-dimensional system) for evaluating hand dysfunction among stroke survivors so as to provide an objective basis for its clinical application.Methods:Sixty stroke survivors with single dysfunctional hand were evaluated using the Multi-dimensional system and also using the Fugl-Meyer Upper Extremity Assessment (FMA-UE) and the Action Research Arm Test (ARAT) and in terms of their active range of hand motion (AROM-H). The Multi-dimensional system′s internal consistency, composite reliability, split-half reliability, parallel-forms reliability as well as intra- and inter-observer reliability were quantified. Its validity was tested in terms of content validity, structural validity, convergent validity and criterion validity.Results:The system′s Cronbach′s α was determined to be 0.86. Its one-dimension composite reliability was 0.939, its split-half reliability coefficient was 0.88 and its parallel-forms reliability was 0.922. The inter-evaluator reliability was between 0.965 and 0.998 [95%CI= (0.919, 0.999)], and the single evaluator ICC value was 0.973 to 0.998 [95%CI= (0.937, 0.999)]. The validity test produced a content validity index of 1, and the Pearson correlation coefficients between each sub-item and the total score were all greater than 0.60. The KMO value for structural validity was 0.882, and there was a common factor in the component matrix which reflected 60.9% of the information in the original variables. The system′s convergent validity AVE was 0.609 with Pearson correlation coefficients between the multidimensional system and the FMA-UE and ARAT both > 0.70.Conclusion:The multi-dimensional quantitative video evaluation system has good reliability and validity in the evaluation of stroke survivors with hand dysfunction.

An integrated strategy for comprehensive characterization of metabolites and metabolic profiles of bufadienolides from Venenum Bufonis in rats / 药物分析学报

Comprehensive characterization of metabolites and metabolic profiles in plasma has considerable sig-nificance in determining the efficacy and safety of traditional Chinese medicine(TCM)in vivo.However,this process is usually hindered by the insufficient characteristic fragments of metabolites,ubiquitous matrix interference,and complicated screening and identification procedures for metabolites.In this study,an effective strategy was established to systematically characterize the metabolites,deduce the metabolic pathways,and describe the metabolic profiles of bufadienolides isolated from Venenum Bufonis in vivo.The strategy was divided into five steps.First,the blank and test plasma samples were injected into an ultra-high performance liquid chromatography/linear trap quadrupole-orbitrap-mass spectrometry(MS)system in the full scan mode continuously five times to screen for valid matrix compounds and metabolites.Second,an extension-mass defect filter model was established to obtain the targeted precursor ions of the list of bufadienolide metabolites,which reduced approximately 39％of the interfering ions.Third,an acquisition model was developed and used to trigger more tandem MS(MS/MS)fragments of precursor ions based on the targeted ion list.The acquisition mode enhanced the acquisition capability by approximately four times than that of the regular data-dependent acquisition mode.Fourth,the acquired data were imported into Compound Discoverer software for identification of metabolites with metabolic network prediction.The main in vivo metabolic pathways of bufadienolides were elucidated.A total of 147 metabolites were characterized,and the main biotransformation reactions of bufadienolides were hydroxylation,dihydroxylation,and isomerization.Finally,the main prototype bufadienolides in plasma at different time points were determined using LC-MS/MS,and the metabolic profiles were clearly identified.This strategy could be widely used to elucidate the metabolic profiles of TCM preparations or Chinese patent medicines in vivo and provide critical data for rational drug use.

Application research of multi-dimensional interaction combined with PBL teaching in emergency and critical care nursing practice teaching / 中华医学教育探索杂志

Objective:To explore the value of multi-dimensional interaction combined with problem-based learning (PBL) teaching in the nursing practice teaching of emergency and critical care.Methods:A total of 110 nursing students who practiced in the First Affiliated Hospital of Xinjiang Medical University from June 2019 to March 2020 were selected as the control group, and the traditional teaching was adopted. In addition, 89 nursing students who practiced from June 2020 to December 2020 were selected as the research group, and multi-dimensional interaction combined with PBL teaching was adopted. The teaching quality was assessed through the comprehensive scores of the nursing students (departure examination + regular performance assessment) and nursing students' feedback on the teaching. SPSS 22.0 was used for t-test and Chi-square test. Results:The comprehensive scores of the nursing students in the research group were higher than those in the control group, and the difference was statistically significant ( P<0.001). Compared with the control group, the nursing students in the research group believed that multi-dimensional interaction combined with PBL teaching could improve nursing knowledge and skills, overall clinical nursing ability, self-management ability, clinical thinking ability, and the ability to analyze, judge and solve clinical nursing problems, group cooperation ability, communication ability between doctors, nurses and patients, emergency ability and rescue ability, and professional quality, with statistically significant differences ( P<0.001). Conclusion:Multi-dimensional interaction combined with PBL teaching can improve the theoretical and clinical practice level of nursing interns, and at the same time help to cultivate nursing talents with excellent comprehensive quality.

Percepción de personas con tartamudez en cuanto a sus experiencias de tratamiento basado en el modelo multidimensional CALMS / Perception of people with stuttering regarding their treatment experiences based on the multidimensional CALMS model

Conocer la percepción de personas con tartamudez sobre sus experiencias de tratamiento en base al modelo multidimensional CALMS en sus cinco dimensiones; cognitiva, afectiva o emocional, lingüística, control motor del habla y social. Esta investigación se desarrolló con un enfoque cualitativo, análisis descriptivo y abordaje fenomenológico. Participaron cinco sujetos de género masculino de entre 19 a 39 años de edad, diagnosticados con tartamudez del desarrollo persistente. Cada uno de ellos respondió a una entrevista semiestructurada de manera individual, a través de preguntas abiertas categorizadas en las cinco dimensiones del modelo multidimensional CALMS. Para el análisis de la información se utilizó el software Atlas.ti versión 7. Los resultados de la encuesta realizada indicaron que los participantes perciben el abordaje multidimensional como un modelo de intervención que les ayuda a desarrollar su confianza, desempeñarse de mejor manera en su vida cotidiana y mejorar su calidad de vida, aunque aún persistan ciclos de sentimientos y pensamientos negativos dentro de los contextos de su vida diaria y entorno en general.A pesar de que los sujetos perciben de una manera positiva la intervención fonoaudiológica, las características personales de cada participante y el impacto del entorno social influyeron en las respuestas del ámbito cognitivo y emocional basadas en las preguntas de la encuesta.

To know the perception of people with stuttering about their treatment experiences based on the CALMS multidimensional model in its five dimensions; cognitive, affective or emotional, linguistic, motor control of speech and social. This research was developed with a qualitative approach, descriptive analysis and phenomenological approach. Five male subjects between 19 and 39 years of age, diagnosed with persistent developmental stutter, participated. Each of them responded to a semi-structured interview individually, through open questions categorized in the five dimensions of the CALMS multidimensional model. The Atlas.ti version 7 software was used for the analysis of the information. The results of the survey indicated that the participants perceive the multidimensional approach as an intervention model that helps them develop their confidence, perform better in their lives and improve their quality of life, even though cycles of negative thoughts and feelings still persist within the contexts of their daily life and environment in general. Although the subjects perceive the speech-language intervention in a positive way, the personal characteristics of each participant and the impact of the social environment influenced the answers in the cognitive and emotional domain based on the questions in the survey.

The multidimensional comprehension of Chagas disease. Contributions, approaches, challenges and opportunities from and beyond the Information, Education and Communication field

Chagas is a complex, multidimensional phenomenon in which political, economic, environmental, biomedical, epidemiological, psychological, and sociocultural factors intersect. Nonetheless, the hegemonic conceptualisation has long envisioned Chagas as primarily a biomedical question, while ignoring or downplaying the other dimensions, and this limited view has reinforced the disease's long neglect. Integrating the multiple dimensions of the problem into a coherent approach adapted to field realities and needs represents an immense challenge, but the payoff is more effective and sustainable experiences, with higher social awareness, increased case detection and follow-up, improved adherence to care, and integrated participation of various actors from multiple action levels. Information, Education, and Communication (IEC) initiatives have great potential for impact in the implementation of multidimensional programs of prevention and control successfully customised to the diverse and complex contexts where Chagas disease persists.

Allometrically adjusted handgrip strength and chair stand test cut points to identify sarcopenia in older Portuguese adults / Pontos de corte da força de preensão manual e teste de sentar e levantar da cadeira ajustados alometricamente para identificar sarcopenia em idosos portugueses

ABSTRACT Absolute muscle strength or adjusted by body mass index (BMI) are useful to identify sarcopenia. However, these values are not accurate for older adults with extreme body sizes because the nonlinear relationship between strength and body size. The purpose was to determine cut-off points for identify sarcopenia in older adults using allometric coefficients to normalise handgrip strength (HGS) and 30-sec chair stand test (30-s CST) by body size. Allometric exponents were proposed with log-linear models for body-size variables (body mass, height and BMI). The remotion of body-size effect on muscle strength with allometric normalisation was tested by partial correlation. Cut-off points for low muscle strength were established by ROC curve and Youden index considering functional limitation (six-minute walk test<400m). Allometric exponents provided for body-size variables range from -0.01 to 2.28 (HGS) and -0.27 to 0.21 (30-s CST). The effect of body size on muscle strength was removed with allometric normalisation (r<0.30). Cut-off points accuracy was always adequate (AUC≥0.78; p<0.001). In conclusion, cut-off points of HGS and 30-s CST allometrically normalised were proposed to identify sarcopenia in Portuguese older adults and allometry maintained adequate the accuracy (AUC>70%). Allometry removed influence of body size on the expression of HGS and 30-s CST and permits evaluate muscle strength regardless of body-size.

RESUMO A força muscular absoluta ou ajustada pelo índice de massa corporal (IMC) é útil para identificar a sarcopenia. No entanto, esses valores não são precisos para idosos com tamanhos corporais extremos devido à relação não linear entre força e tamanho corporal. O objetivo foi determinar os pontos de corte para identificar a sarcopenia em idosos usando coeficientes alométricos para normalizar a força de preensão manual (FPM) e teste de sentar e levantar da cadeira em 30 segundos (30-s CST) por tamanho corporal. Os expoentes alométricos foram propostos com modelos log-lineares para variáveis de tamanho corporal (massa corporal, estatura e IMC). A remoção do efeito do tamanho corporal na força muscular com normalização alométrica foi testada por correlação parcial. Os pontos de corte para baixa força muscular foram estabelecidos pela curva ROC e índice de Youden considerando a limitação funcional (teste de caminhada de seis minutos<400m). Os expoentes alométricos fornecidos para variáveis de tamanho corporal variam de -0,01 a 2,28 (FPM) e -0,27 a 0,21 (30-s CST). O efeito do tamanho corporal na força muscular foi removido com normalização alométrica (r<0,30). A precisão dos pontos de corte sempre foi adequada (AUC≥0,78; p<0,001). Em conclusão, foram propostos pontos de corte para FPM e 30-s CST normalizados alometricamente para identificar sarcopenia em idosos portugueses e a alometria manteve a precisão adequada (AUC>70%). A alometria removeu a influência do tamanho corporal na expressão da FPM e 30-s CST e permite avaliar a força muscular independentemente do tamanho corporal.

Una aproximación a la alfabetización nutricional, evaluación del estado nutricional y calidad de la alimentación en una muestra a conveniencia de mujeres de comunas con pobreza multidimensional

RESUMEN La obesidad es factor de riesgo para las enfermedades crónicas no transmisibles. Esta condición afecta principalmente a mujeres de bajo nivel socioeconómico. La alfabetización nutricional, referida al conocimiento en alimentación y nutrición, podría estar determinando la calidad de la dieta, la que a su vez podría favorecer el desarrollo de la obesidad. Los objetivos del estudio fueron 1) Describir la alfabetización nutricional, el estado nutricional y la calidad de la alimentación, y 2) Explorar la relación entre estas tres variables, en un grupo de mujeres de comunas con pobreza multidimensional de la Región Metropolitana. Se diseñó un estudio transversal, no probabilístico, en donde participaron 122 mujeres. Se evalúo el estado nutricional mediante el cálculo del Índice de Masa Corporal, midiendo peso y talla. La calidad de la alimentación se evaluó utilizando una encuesta de tendencia de consumo alimentaria y el Índice de Alimentación Saludable, y la alfabetización nutricional, con el cuestionario Nutritional Literacy en español (NLit-S), adaptado a la población chilena. La prevalencia de obesidad fue 47,5% y el 88,5% de las mujeres presentó necesidad de cambios en su alimentación. Un 66,4% presentó una alfabetización nutricional deficiente, con más mujeres con exceso de peso en esta categoría. Dominio de etiquetado nutricional fue el que obtuvo peores resultados. Se concluye que la condición alimentaria nutricional de las mujeres de nuestro estudio sugiere un desafío para el trabajo por parte de profesionales de la salud, así como para quienes toman decisiones en alimentación y nutrición.

ABSTRACT Obesity is a risk factor for chronic non-communicable diseases. This condition mainly affects women of low socioeconomic status. Nutritional literacy, understood as knowledge of food and nutrition, could determine the quality of the diet, which in turn could cause the development of obesity. The aims were 1) To describe the nutritional literacy, nutritional status, and food quality, and 2) To explore the relationship between these three variables, in a group of women from communes with multidimensional poverty in the Metropolitan Region. We conducted a cross-sectional, non-probabilistic study in which 122 women participated. The nutritional status was evaluated by calculating the Body Mass Index using weight and height measurements. The diet quality was evaluated using a survey of food consumption trends and the Healthy Eating Index. Nutritional literacy was evaluated with the Nutritional Literacy questionnaire in Spanish adapted for the Chilean population. The prevalence of obesity was 47.5%, and 88.5% of the women presented the need for changes in their diet. 66.4% of women had low nutritional literacy, with more overweight women in this category. The nutrition labeling domain was the one with the worst results. We concluded that women's food and nutritional condition suggests a challenge for health professionals and those who make decisions about food and nutrition.