ABSTRACT
Multiple myeloma (MM) originates from malignant plasma cells, leading to multiple destructive lytic bone lesions that occur in more than 80%of MM patients. MicroRNAs have been reported to be involved in the development of bone lesions in MM. However, it is still unclear that microRNAs can be considered as diagnostic and prognostic biomarkers for bone lesions. MiR-214 and miR-135b may participate in the pathogenesis of bone marrow, which has shown a certain guiding significance in its prognosis. This paper will introduce the relationship between miR-214, miR-135b and myeloma bone disease.
ABSTRACT
Objective To investigate the relationship between macrophage inflammatory protein 1-α( MIP-1α) ,sclerostin and multiple myeloma bone disease( MBD) .Methods 52 patients with multiple myeloma were selected as the observation group,among them,9 cases without MBD,43 cases with MBD;MBD grade:9 cases with grade 0,8 cases with grade 1,21 cases with grade 2,14 cases with grade 3.25 healthy examinee were selected as the control group.MIP-1αand sclerostin were observed.Results MIP-1α,sclerostin in the observation group[(158.43 ± 78.75)pg/mL,(0.62 ±0.24)pg/mL] were higher than those in the control group[(52.46 ±21.35)pg/mL,(0.31 ± 0.14)pg/mL](t =9.635,P <0.05;t =8.844,P <0.05);MIP-1α,sclerostin of MBD patients[(175.55 ± 62.75)pg/mL,(0.84 ±0.54)pg/mL]were higher than those of non MBD patients[(89.83 ±41.57)pg/mL,(0.42 ± 0.25)pg/mL](t=7.665,P<0.05;t=6.834,P<0.05).There were positive relation between MIP-1α,sclerostin and the grade of MBD(r=0.572,P<0.05;r=0.683,P<0.05).There was positive relation between the expressions of MIP-1αand sclerostin(r =0.522,P <0.05).Conclusion MIP-1αand sclerostin of patients with MM have increased.There is a significant relation between MIP-1α, sclerostin and MBD.United detection of MIP-1αand sclerostin may be an index used as evaluation of MBD.
ABSTRACT
ObjectiveTo detect IL-17 level of bone marrow in patients with multiple myeloma bone disease,and to investigate its clinical significance.MethodsThe bone marrow IL-17 levels were quantified in 33 cases of multiple myeloma patients and 20 normal control by ELISA assay. RANKL mRNA expression were detected by using RT-PCR.ResultsIn bone marrow supernatant,IL-17 was detected in both groups,and RANKL mRNA were detected in both groups too. IL-17 and RANKL mRNA levels in bone marrow of patients with MM were significantly higher than those in the control group(P＜0.05). The bone marrow concentrations of IL-17 and bone marrow mononuclear cells' RANKL mRNA expression in active stage were significantly higher than those in stable stage (P＜0.05).The bone marrow IL-17 and RANKL were significantly correlated (r =690,P＜0.05).ConclusionIL-17 may play an important role in the pathogenesis of MM.
ABSTRACT
ObjectiveTo investigate the gene expressions of TAZ and Wnt/β-catenin on the postosteogenic cells of mesenchymal stem cells(MSC)in multiple myeloma(MM)patients and to explore the potential therapeutic target of multiple myeloma bone disease (MBD).MethodsBone marrow mononuclear cells MNC from MM and controls were isolated,cultured,expanded and then induced to osteogenic differentiation.Realtime quantitative RT-PCR was employed to detect the osteogenic markers (TAZ,Wnt/β-catenin,OPN,OC,ALP and Cbf α1); and alizarin red staining for mineral deposition.The mRNA expressions of TAZ and Wnt/β-catenin in the two groups were analysed.ResultsAlizarin red staining was positive and the red calcium nodules were appeared on the post-osteogenic cells of MSC.The mRNA expressions of OC,ALP and Cbf α1 were 2.0958±0.5665,2.6670±0.3847,0.8463±0.3473,respectively,on the post-osteogenic cells of MSC in the experimental groups,which were significantly higher than those of pre-osteogenic cells(1.3487±0.9291,1.1452±0.6054,0.4439±0.2945) (t =2.171,6.709,2.795; all P ＜ 0.05).The mRNA expressions of OPN,OC,ALP and Cbf α1 were 2.1096±0.8267,2.8991±0.3531,4.3045±0.2844,1.3273±0.4075,respectively,on the post-osteogenic cells of MSC in the controls,which were significantly higher than those of pre-osteogenic cells (1.2200±0.9091,0.8780±0.3927,1.9161±0.2684,0.6736±0.2513) (t =2.289,12.103,25.134,4.411; all P ＜ 0.05).The mRNA expressions of OPN,OC,ALP,Cbf α1 were 1.2710±0.5636,2.0958±0.5665,2.6670± 0.3847,0.8463+0.3473,respectively,on the post-osteogenic cells of MSC in the experimental groups,which were significantly lower than those of control groups(2.1096 ±0.8267,2.8991 ±0.3531,4.3045±0.2844,1.3273±0.4075) (t =-2.650,-3.805,-10.822,-2.841; all P ＜ 0.05).The mRNA expression of TAZ and β-catenin were 2.2315±1.0723 and 0.5801±0.2159 on the post-osteogenic cells of MSC in MM patients,which were significantly lower than those of control groups (4.4140±0.8325,0.9516±0.2920) (t =±5.085,-3.235;both P ＜ 0.05).ConclusionThe gene expressions of OPN,OC,ALP and Cbf α1,the osteogenesis related genes,are increased in post-osteogenic cells of MSC,which showed the MSC have been successfully induced to osteoblasts.Comparing with control groups,the osteogenic potential of MSC in MM patients is lower.Based on the above research,TAZ and Wnt/β-catenin may present a novel target for the future therapy of MBD.