Bergson e a clínica psicanalítica / Bergson and the psychoanalytical clinic / Bergson e la clínica psicanalítica

O presente artigo procura conjugar aspectos da filosofia bergsoniana com determinantes fundamentais do pensamento analítico, avaliando assim o benefício clínico e ético de suas interações. Para tanto, fará a investigação incidir, especialmente, sobre as noções de "impulso vital" e de "pulsão". O retorno a si, a multiplicidade virtual, os atos livres, a decifração pragmática da força, são temas que se interpenetram de tal modo nas duas experiências do pensamento, que suas intersecções geram - assim o pretendemos - abordagens mais nuançadas dos problemas analíticos, compreendendo uma retomada essencial da perspectiva ética, não mais situada a propósito do inconsciente, mas constituindo o seu plano

This paper combines aspects of Bergsonian philosophy with fundamental determinants of analytical thinking, thus evaluating the clinical and ethical benefits of their interactions. To do so, the investigation will focus, especially, on the notions of "vital impulse" and "drive". The return to oneself, the virtual multiplicity, the free acts, the pragmatic deciphering of force, are themes that interpenetrate themselves in such a way in the two experiences of thought, that their intersections generate - as intended - more nuanced approaches to analytical problems, involving an essential resumption of the ethical perspective, no longer situated in relation to the unconscious, but constituting its plan

Este artículo busca combinar aspectos de la filosofía bergsoniana con determinantes fundamentales del pensamiento analítico, evaluando así los beneficios clínicos y éticos de sus interacciones. Por tanto, la investigación se centrará en las nociones de "impulso vital" y "pulsión". El retorno a uno mismo, la multiplicidad virtual, los actos libres, el desciframiento pragmático de la fuerza, son temas que se interpenetran de tal manera en las dos experiencias de pensamiento, que sus intersecciones generan - como pretendo - aproximaciones más matizadas a problemas analíticos, involucrando una reanudación esencial de la perspectiva ética, que ya no se sitúa en relación con el inconsciente, sino que constituye su plan.

Texto descrente: em defesa das multiplicidades na Educação Física / Unbelievable text: in defense of multilicities in Physical Education / Texto increíble: en defensa de las multilicidades en la Educación Física

O presente artigo, de tipo ensaístico, lança mão daquilo que chama de descrença associada à Educação e, mais especificamente, à Educação Física, para defender as multiplicidades, inspirados pelos escritos de Deleuze e Guattari (2011a, 2011b, 2012a, 2012b). A descrença torna-se uma espécie de primeira fase para a aposta na multiplicidade, visto que ao desacreditar a representação, aquilo que está além dos(as) docentes, dos (as) alunos (as) e da escola, passa-se a crer naquilo que se vive aqui e agora, no território em que se habita. Visando lançar o olhar para os problemas educacionais a fim de rechaçar a representação que assola o pensamento escolar e que faz reverberar os velhos cacoetes necessários ao mercado de trabalho ­ habilidades, competências ­, este texto objetiva instigar uma docência que assuma uma postura cartográfica, mais exploratória e menos decalcadora.

This essay, of an essay type, makes use of what it calls disbelief associated with Education and, more specifically, with Physical Education, to defend multiplicities, inspired by the writings of Deleuze and Guattari (2011a, 2011b, 2012a, 2012b). Disbelief becomes a kind of first phase for the bet on multiplicity, since by discrediting representation, what is beyond the teachers, the students and the school, one starts to believe in what is lives here and now, in the territory in which one inhabits. Aiming to cast a look at educational problems in order to reject the representation that plagues school thinking and that makes the old taunts necessary for the job market ­ skills, competences ­ reverberate, the text aims to instigate a teaching that assumes a cartographic posture, more exploratory and less tracing.

Este ensayo, de tipo ensayístico, se sirve de lo que denomina descreimiento asociado a la Educación y, más concretamente, a la Educación Física, para defender las multiplicidades, inspirándose en los escritos de Deleuze y Guattari (2011a, 2011b, 2012a, 2012b). El descreimiento se convierte en una especie de primera fase para la apuesta por la multiplicidad, ya que al desprestigiar la representación, lo que está más allá de los docentes, los alumnos y la escuela, se pasa a creer en lo que se vive aquí y ahora, en el territorio que se habita. Con el objetivo de lanzar una mirada a los problemas educativos para rechazar la representación que plaga el pensamiento escolar y que hace resonar las viejas burlas necesarias para el mercado de trabajo ­ habilidades, competencias ­, el texto pretende instigar una enseñanza que asume una postura cartográfica, más exploratorio y menos rastreador.

Multiplicity of infection of Plasmodium knowlesi in Malaysia: an application of Pkmsp-1 block IV

@#In Malaysia presently, the main cause of human malaria is by the zoonotic monkey parasite Plasmodium knowlesi. A previous study has suggested that the P. knowlesi merozoite surface protein 1 (Pkmsp-1) block IV to be a suitable multiplicity of infection (MOI) genotyping marker for knowlesimalaria. This study therefore aimed to investigate the usefulness of Pkmsp-1 block IV in assessing the MOI of P. knowlesi in clinical isolates from Malaysia. Two allele-specific PCR primer pairs targeting the two allelic families of block IV (T1 and T2) were designed, and used to genotype P. knowlesi in 200 blood samples (100 from Peninsular Malaysia and 100 from Malaysian Borneo). Results showed that the mean MOI in Malaysian Borneo was slightly higher as compared to Peninsular Malaysia (1.58 and 1.40, respectively). Almost half of the total blood samples from Malaysian Borneo (52%) had polyclonal infections (i.e., more than one allele of any family type) as compared to Peninsular Malaysia (33%) samples. The T1 allelic family was more prevalent in Peninsular Malaysia (n=75) than in Malaysian Borneo (n=60). The T2 allelic family, however, was more prevalent in the Malaysian Borneo (n=87 vs n=53 respectively). This study shows that the single locus Pkmsp-1 block IV can serve as a simple alternative genetic marker for estimating knowlesi malaria MOI in a population. Future MOI studies should focus on macaque populations as macaques are the natural host of P. knowlesi.

El amor en Badiou: ontologia del vacío, la no relación sexual y la verdad / Love in Badiou: ontology of void, non-sexual relationship and truth / O amor em Badiou: ontologia do vazio, da não-sexualidade e da verdade

RESUMEN El objetivo del siguiente artículo de reflexion es ofrecer una lectura del concepto de amor en el pensamiento de Alain Badiou que medie y armonice el trasfondo abstracto y formal de su metaontología con sus conocidas intervenciones sobre este concepto dirigidas al gran público. Se llena con ello un vacío en la literatura que analiza esta region de su pensamiento. El artículo acomete esta tarea en tres momentos: primero se explica la conexión, que se asume que hay entre la teorïa del ser como multiplicidad y la teona lacaniana de la sexuación a fin de senalar la continuidad y limite entre las mismas. Tras ello, situamos el amor en las coordenadas de la teoría del acontecimiento, el sujeto y la verdad. Finalmente, interpretamos la propuesta de Badiou a la luz de algunos elementos que proceden de la teoría del amor de Platon y de lo que llamamos un comunismo de la diferencia o mínimo.

AВSTRAСT The aim of the current reflection article is to offer a reading of the concept of love in Alain Badiou's thought that mediates and harmonizes the abstract and formal background of his metaontology with his well-known interventions on this concept aimed at the general public. This fills a gap in the literature that analyzes this region of his thinking. The article undertakes this task in three moments: First the connection, which is assumed to exist, between the theory of being as multiplicity and the Lacanian theory of sexuation is explained in order to indicate the continuity and limit between them. After that, we place love in the coordinates of the theory of the event, the subject and the truth. Finally we interpret Badiou's proposal in the light of some elements that come from Plato's theory of love and what we call a communism of difference or minimum.

RESUMО O objetivo do artigo de reflexão a seguir é oferecer uma leitura do conceito de amor no pensamento de Alain Badiou que medeia e harmoniza o fundo abstrato e formal de sua metaontologia com suas conhecidas intervenções sobre este conceito dirigidas ao público em geral. Isto preenche uma lacuna na literatura que analisa esta região de seu pensamento. O artigo empreende esta tarefa em três etapas: primeiro, a suposta conexão entre a teoria do eu como multiplicidade e a teoria lacaniana da sexuação é explicada a fim de apontar a continuidade e os limites entre elas. Em seguida, situamos o amor nas coordenadas da teoria do evento, do assunto e da verdade. Finalmente, interpretamos a proposta de Badiou á luz de alguns elementos que provêm da teoria do amor de Platão e do que chamamos um comunismo de diferença ou minimalismo.

Multivariate analysis of the operative effect on cervical spinal cord injury without fracture or dislocation / 中国骨伤

OBJECTIVE@#To explore the influencing factors of the operative effect on cervical spinal cord injury without fracture or dislocation.@*METHODS@#The clinical data of 69 patients with cervical spinal cord injury without fracture or dislocation from November 2010 to November 2016 who received operation were retrospectively analyzed. There were 37 males and 32 females, aged from 32 to76 years with an average of (51.6±7.3) years. The clinical data of 12 factors were selected, including age, gender, ASIA grade of spine cord injury, the length of spine cord injury by MRI, Pavlov ratio, ossification of the posterior longitudinal ligament (OPLL), intervertebral disc herniation, type of spine cord injury by MRI, time from injury to operation, treatment of high-dose methylprednisolone, operation time, intraoperative bleeding volume. In order to screen the main influencing factors of above items to prognosis, the single factor and multiple factor Logistic regression analysis were used in the clinical data by SPSS 22.0 statistical software.@*RESULTS@#Univariate analysis results showed that the factors including ASIA grade of spine cord injury, the length of spine cord injury by MRI, Pavlov ratio, ossification of longitudinal ligament, intervertebral disc herniation, the type of spine cord injury by MRI were associated with prognosis (<0.05). Multi factor analysis of the selected factors indicated that the type of spine cord injury by MRI, the length of spine cord injury by MRI, Pavlov ratio, ASIA grade of spine cord injury were the main prognostic factors according to the influence intensity (<0.05).@*CONCLUSION@#The influencing factors of the operative effect on cervical spinal cord injury without fracture and dislocation were the type of spine cord injury by MRI, the length of spine cord injury by MRI, Pavlov ratio, ASIA grade of spine cord injury, and the foremost were the type and length of spine cord injury by MRI. Compared with other patients, preoperative MRI showed the patient with spinal cord injury type with bleeding and edema, or the length of spine cord injury larger than 45 mm may be less effective, therefore, it is necessary to thoroughly communicate with the patients and their kin before surgery.

Multivariate analysis of short-term prognosis of liver transplantation in patients with end-stage liver disease / 中华器官移植杂志

Objective To analyze many indicators during perioperative period of liver transplantation in patients with end-stage liver disease ,only to seek related factors that can accurately predict short-term(≤ three months)outcome .Methods We analyzed retrospectively clinical data of consecutive patients with end-stage liver diseases undergone liver transplantation in a single treatment center .Logistic regression analysis was used to analyze the perioperative indicators including recipient gender ,age ,body mass index ,preoperative serum albumin level ,serum sodium concentration ,urea nitrogen level and donor-recipient blood group ,et al .Correlated factors were analyzed by the method of multivariate logistic regression .Statistical processing package was SAS 9 .1 .3 soft .The difference was statistically significant with P< 0 .05 .Results 18/165 patients died within 3 months after transplantation(mortality rate :10 .9 ％ ) .According to the result of univariate analysis ,the indicators correlated with early mortality which were statistically significant were preoperative serum sodium , blood urea nitrogen ,PT-INR ,CTP score ,MELD score and MELD-Na score .On the base of the result of Logistic multiple regression . However ,only MELD-Na score was associated with 3 months prognosis(P=0 .001 ,β=-2 .510 ,OR=0 .088 ,95 ％ CI=0 .037～0 .349) .Conclusions Preoperative MELD-Na score is an independent risk factor for short-term survival in patients with end-stage liver disease .Higher MELD-Na score is ,the early mortality is higher .

Multivariate analysis of short-term prognosis of liver transplantation in patients with end-stage liver disease / 中华器官移植杂志

Objective@#To analyze many indicators during perioperative period of liver transplantation in patients with end-stage liver disease, only to seek related factors that can accurately predict short-term(≤three months)outcome.@*Methods@#We analyzed retrospectively clinical data of consecutive patients with end-stage liver diseases undergone liver transplantation in a single treatment center. Logistic regression analysis was used to analyze the perioperative indicators including recipient gender, age, body mass index, preoperative serum albumin level, serum sodium concentration, urea nitrogen level and donor-recipient blood group, et al. Correlated factors were analyzed by the method of multivariate logistic regression. Statistical processing package was SAS 9.1.3 soft. The difference was statistically significant with P<0.05.@*Results@#18/165 patients died within 3 months after transplantation(mortality rate: 10.9 %). According to the result of univariate analysis, the indicators correlated with early mortality which were statistically significant were preoperative serum sodium, blood urea nitrogen, PT-INR, CTP score, MELD score and MELD-Na score. On the base of the result of Logistic multiple regression. However, only MELD-Na score was associated with 3 months prognosis(P=0.001, β=-2.510, OR=0.088, 95 % CI=0.037~0.349).@*Conclusions@#Preoperative MELD-Na score is an independent risk factor for short-term survival in patients with end-stage liver disease. Higher MELD-Na score is, the early mortality is higher.

Study of light-particle multiplicities in p + non-fissionable nuclei events in the 0.5 - 2 GeV energy range / Estudio de la multiplicidad de partículas ligeras en proto-reacciones con núcleos no fisionables en el rango energético 0.5 - 2 GeV

Abstract In recent years, the investigation of spallation reactions have caught the attention of scientific community due to their application in the transmutation of nuclear waste by using the Accelerator Driven System (ADS) reactors. Due to the experimental difficulties that nuclear reactions researches face; the study of spallation reaction by using simulation codes is more suitable for generating more complete database for different energy ranges. This work aims to study spallation reactions induced by protons at intermediate energies 0.5 - 2 GeV on non-fissionable nuclei by using the Monte Carlo code: CRISP (Collaboration Rio-Ilhéus-São Paulo). The target nuclei studied were: 184 W, 197 Au and 208 Pb, focusing on the last one. Multiplicity of light particles obtained with CRISP was compared with the available experimental data and other Monte Carlo codes involved in the study of spallation reactions, resulting on a quite satisfactory agreement.

Resumen En años recientes la investigación de las reacciones nucleares de "espalación" han causado atención en la comunidad científica debido a su aplicación en la transmutación de los residuos nucleares usando reactores ADS (AcceleratorDriven System). Debido a las dificultades experimentales en el estudio de las reacciones nucleares, su estudio vía simulación es adecuado para generar una base de datos más completa en un amplio rango de energía. Este trabajo tiene como objetivo principal el estudio de reacciones nucleares inducidas por protones a energías intermedias, 0.5 - 2 GeV, en núcleos no fisionables, utilizando el código de Monte Carlo: CRISP (Collaboration Rio-Ilhéus-São Paulo). Los núcleos estudiados fueron: 184W, 197Au y 208Pb, con un enfoque en el último. La multiplicidad de partículas ligeras obtenida con el CRISPfue comparada con los datos experimentales disponibles y con otros códigos de Monte Carlo y se obtuvieron resultados satisfactorios.

Control de falsos descubrimientos en mapeo asociativo con poblaciones estructuradas / False discovery rate control in association mapping with genetically structured populations

Las pruebas de asociación entre marcadores moleculares y variables fenotípicas son cruciales para la identificación de QTL (Quantitative Trait Loci). Los avances biotecnológicos incrementaron la disponibilidad de marcadores genéticos y consecuentemente el número de pruebas de la asociación fenotipo-genotipo. El incremento de pruebas de significancia estadística a realizar en simultaneo (multiplicidad) demanda correcciones de los valores-p obtenidos para cada prueba de hipótesis de manera de mantener acotada las tasas de error para la familia de pruebas de asociación. Las correcciones estadísticas clásicas para el problema de multiplicidad, como Bonferroni, el método de control de la tasa de falsos descubrimientos (FDR) y el número efectivo de pruebas (Meff), son ampliamente usadas, pero fueron desarrolladas para datos independientes. Sin embargo, cuando las poblaciones de mapeo están genéticamente estructuradas los datos dejan de ser independientes. En este trabajo, proponemos un método de corrección por multiplicidad basado en estimación del número efectivo de pruebas desde un modelo que ajusta por la estructura de correlación subyacente. Se evalúa el desempeño del procedimiento propuesto a través del análisis de los valores-p obtenidos para un conjunto de QTL simulados. Los resultados sugieren que el método propuesto provee control de la tasa de falsos positivos y presenta mayor potencia que otros métodos de corrección por multiplicidad usados en mapeo asociativo.

The association tests between molecular markers and phenotypic traits are crucial for the Quantitative Trait Loci (QTL) identification. Biotechnological advances increased the molecular marker information; consequently, the number of genotype-phenotype association tests required incremented too. The multiple statistical inferences (multiplicity) demand corrections of the p-values obtained for each comparison in order to keep limited the error rates for the family of association tests. However, classic statistical correction methods such as Bonferroni, False Discovery Rate (FDR) and the Effective Number of Independent Test (Meff) were developed in the context of independent data. Wherever, when the population genetic structure is present, the data are no longer independent. In this paper, we propose a method of correction for multiplicity based on estimation of the effective number of tests from a model that adjust for the underlying correlation structure. We evaluate the performance of the proposed procedure in the estimation of p-values for a set of simulated QTL. The results suggest that the proposed method provides control of FDR and has more power than other methods for multiplicity correction used in association mapping.

The Prognostic Impact of Synchronous Ipsilateral Multiple Breast Cancer: Survival Outcomes according to the Eighth American Joint Committee on Cancer Staging and Molecular Subtype

BACKGROUND: In the current American Joint Committee on Cancer staging system of breast cancer, only tumor size determines T-category regardless of whether the tumor is single or multiple. This study evaluated if tumor multiplicity has prognostic value and can be used to subclassify breast cancer. METHODS: We included 5,758 patients with invasive breast cancer who underwent surgery at Samsung Medical Center, Seoul, Korea, from 1995 to 2012. RESULTS: Patients were divided into two groups according to multiplicity (single, n = 4,744; multiple, n = 1,014). Statistically significant differences in lymph node involvement and lymphatic invasion were found between the two groups (p < .001). Patients with multiple masses tended to have luminal A molecular subtype (p < .001). On Kaplan-Meier survival analysis, patients with multiple masses had significantly poorer disease-free survival (DFS) (p = .016). The prognostic significance of multiplicity was seen in patients with anatomic staging group I and prognostic staging group IA (p = .019 and p = .032, respectively). When targeting patients with T1-2 N0 M0, hormone receptor–positive, and human epidermal growth factor receptor 2 (HER2)–negative cancer, Kaplan-Meier survival analysis also revealed significantly reduced DFS with multiple cancer (p = .031). The multivariate analysis indicated that multiplicity was independently correlated with worse DFS (hazard ratio, 1.23; 95% confidence interval, 1.03 to 1.47; p = .025). The results of this study indicate that tumor multiplicity is frequently found in luminal A subtype, is associated with frequent lymph node metastasis, and is correlated with worse DFS. CONCLUSIONS: Tumor multiplicity has prognostic value and could be used to subclassify invasive breast cancer at early stages. Adjuvant chemotherapy would be necessary for multiple masses of T1–2 N0 M0, hormone-receptor-positive, and HER2-negative cancer.

Establishment of a HEK293T cell line able to site-specifically integrate and stably express GDNF by rAAV-2 vector

Background: Using recombinant adeno-associated virus 2 (rAAV-2), we attempted to establish a HEK293T cell line that is able to site-specifically integrate and stably express glial cell line-derived neurotrophic factor (GDNF). Results:Recombinant vector with enhanced green fluorescent protein (EGFP) and GDNF (pTR-P5-EGFP-IRES-GDNF), as well as that carrying Rep genes and SV40 promoters (pSVAV2) were constructed and packed. HEK293T cells were co-infected with rAAV-2/EGFP-GDNF and rAAV-2/SVAV2 virus separately at 1 x 10(4),1 x 10(5),and 1x10(6) of multiplicity of infection (MOI). The efficiency of transduction was detected using flow cytometry. Additionally, the infected HEK293T cells were separately validated by touchdown polymerase chain reaction (PCR) and Western-blot. After 72 h of transduction, the rate of EGFP positive cell was 22%, 45% and 49% at the MOIs of 1 x 10(4),1 x 10(5) and 1 x 10(6), respectively. On the 3rd, 6th and 9th day of cell passage, there was no significant difference in the cell viability and proliferation rate between transduction and control groups. Importantly, touchdown PCR showed that there was a specific PCR amplified product band in the lane of infected cells. Furthermore, GDNF expression was detected in the infected cells after 15 and 180 d of cultivation. Conclusions: A HEK293T cell line able to site-specifically integrate and stably express GDNF was established.

Construction of RIP140 recombinant adenovirus and its expression in neonatal rat cardiomyocytes / 中国药理学通报

Aim The limited transfection efficiency for plasmid in primary neonatal rat cardiomyocytes,which are terminal differentiated cells,and long foreign DNA (the RIP140 gene sequence are as long as 3.5 kb) cause us to choose a better system to study RIP140 gene expression in primary non-replicative cells. Methods Full-length of RIP140 was cloned into pAdTracker-CMV shuttle vector,and then recombined with virus backbone pAdEasy-1 vector in BJ5183 bac-teria.Positive recombinant plasmid was confirmed by sequence analysis and restriction enzyme determina-tion,and then transfected into AD293 cells for amplifi-cation.Titers of virus particles were determined by Tis-sue Culture Infectious Dose 50 (TCID50 )method and cell vitality was analyzed by CCK-8 kit in cardiomyo-cytes.RIP140 gene was identified by Western blot. Results Sequence analysis suggested that full-length RIP140 gene was cloned correctly into AdEasyTM sys-tem.Virus titers of Ad-RIP140 and Ad-GFP were 1011.3 and 1011.7 PFU·mL-1 ,respectively.Cell vitali-ty was not affected when the Multiplicity of Infection (MOI)was lower than 200.Green fluorescent protein (GFP)and Western blot analysis showed RIP140 gene was remarkably increased in cardiomyocytes for 12h in-fection by Ad-RIP140 (P<0.05 ).Conclusion Re-combinant adenovirus containing RIP140 gene was suc-cessfully constructed and effectively expressed in car-diomyocytes.These will be helpful for further research on the function of RIP140 in cardiomyocytes.

La multiplicación dramática en la enseñanza universitaria de lo grupal / The use of dramatic multiplication in university group education / A multiplicação dramática no ensino universitário no âmbito grupal

Este artículo presenta un modo de trabajo característico de la Cátedra de Teoría y Técnica de Grupos de la Facultad de Psicología de la Universidad de Buenos Aires, denominado Jornada de Producciones Grupales. Dicho dispositivo pedagógico fue diseñado para el aprendizaje grupal a través de una experiencia colectiva prolongada de 8 horas, organizada alrededor de un recurso psicodramático: la Multiplicación Dramática. El dispositivo diseñado, en tanto crea condiciones para poner de relevancia la diversidad, hace necesarias algunas consideraciones metodológicas, filosóficas y políticas sobre las nociones de multiplicidad y diferencia. Estas nociones habilitan no sólo categorías, sino prácticas y procedimientos que abran visibilidad y por ende enunciabilidad a la diversidad de diversidades de un colectivo acción.

This article presents a work model applied by the Group Theory and Method, in Faculty of Psychology of the University of Buenos Aires: the Group Productions Session, pedagogical device designed for group learning through extended collective experience of 8 hours which is organized around a psychodramatic resource: the dramatic multiplication. The designed device, as long as it creates conditions to put relevant diversity, needs some methodological, philosophical and political considerations on the concepts of multiplicity, difference, diversity that enable not only categories, but practices, procedures that open visibility and hence enunciability the diversity of diversities of a collective action.

Este artigo apresenta um modelo de trabalho característico da disciplina Teoria e Técnica de Grupo, da Faculdade de Psicologia da Universidade de Buenos Aires: a Jornada de Produções Grupais, dispositivo pedagógico concebido para a aprendizagem em grupo por meio de uma experiência coletiva de oito horas, organizada em torno de um recurso psicodramático: Multiplicação Dramática. Esse dispositivo cria condições para apresentar a relevância da diversidade, com algumas considerações metodológicas, filosóficas e políticas sobre os conceitos de multiplicidade, diferença, diversidade que permitem não só categorias, mas as práticas e os procedimentos que deem visibilidade e, portanto, enunciabilidade à diversidade das diversidades de uma ação coletiva.

Optimize multiplicity of infection and selected concentration of antibiotics in construction of monoclonal stable cell line by lentivirus vector-mediated RNA interence silenced gene SGMS2 / 中国生化药物杂志

Objective To optimize multiplicity of infection ( MOI) and antibiotics ( blasticidin) concentration selecting BSD gene in construction of monoclonal stable cell line by lentivirus vector-mediated RNA interence silenced gene SGMS2.Methods The INS-1 cells were transfected by fluorescence labeled negative control SGMS2-siRNA lentivirus at MOI of 0, 10, 30, 60 and 120 TU number/cell.The cells were photographed under fluorescent microscopy after 72 h cultivation, then fluorescence ratio and apoptosis rate were calculated to determine optimal MOI.The INS-1 cells were treated by blasticidin with different concentrations of 0, 1, 2, and 3 μg/mL, and the apoptosis rate was observed to acquire optimal concentration of antibiotics.The INS-1 cells were transfected by negative control SGMS2-siRNA lentivirus and SGMS2-siRNA lentivirus (virus titer:1 ×108TU/mL) at optimal MOI and positive-transfected cells were selected by blasticidin at optimal concentration, then mixed cell lines were acquired.The monoclonal cell line was constructed at fluorescence ratio of 90%.Results The optimal MOI was 60 with 100% fluorescence ratio, less than 0.5% apoptosis rate and keep original cellular morphology.The optimal concentration of blasticidin was 2 μg/mL with cell adherence disappear and all cells apoptosis.The Ct value of INS-1-SEMS2 cells detected at the second time was 28.21, which was greater than 27.58 at the first time.The interfering efficiency of siRNA was 77.78% which indicated a successful expression of siRNA and construction of monoclonal stable cell line ( INS-1-SEMS2 ).Conclusion The monoclonal stable cell line was successfully constructed by lentivirus vector-mediated RNA interence silenced gene SGMS2.

Analysis of 63 patients with laryngeal carcinoma / 实用医学杂志

Objective To investigate the survival rate and its prognostic factors of laryngeal carcinoma. Methods We retrospectively analyzed 63 cases of laryngeal carcinoma. A total of 7 clinicopathologic factors were studied by univariate analysis and Cox multivariate model. Results The overall cumulative survival rate was 69.8% at 3 years,54.0%at 5 years.In univariate analysis, the survival was related to location of the tumor(P 0.05). In Cox multivariate model, T status (P < 0.01) and N status (P < 0.01) were independent prognostic factors. Conclusions T and N status were independent prognostic factors for patients with laryngeal carcinoma. Early detection and treatment should be given to improve the survival of patients.

Sem centro, no meio / With no center, in the middle / Sin centro, en el medio

O trabalho propõe uma diferença entre pensar nos vínculos e a partir dos vínculos. Nessa segunda opção, é necessário incluir a lógica da multiplicidade, um pensamento rizomático, ou seja, que não coloque o centro nem no sujeito, nem na história, nem no inconsciente, mas sim que transite pelo Entre, a partir do meio. Entre como produção vincular. Essas ideias são trabalhadas a partir do relato de uma consulta familiar.

The paper proposes that there is a difference between thinking of the links and thinking with the links as a starting point. In the latter, it is necessary to include the logic of multiplicity, a rhizomatic thought; in other words, one which doesn't place the center in the subject, in the story, nor in the unconscious, but instead which travels through the Between, from the middle. Between being link production. These ideas are developed from the report of a session of family therapy.

El trabajo propone una diferencia entre pensar en los vínculos y a partir de los vínculos. En esta segunda opción es necesario incluir la lógica de la multiplicidad, un pensamiento rizomático, es decir, que no coloque el centro ni en el sujeto, ni en la historia, ni en el inconsciente, sino que transite por el Entre, desde el medio. Entre como producción vincular. Se trabajan estas ideas a partir del relato de una consulta familiar.

Genetic diversity and complexity of Plasmodium falciparum infections in Lagos, Nigeria

<p><b>OBJECTIVE</b>To analyse the genetic diversity of Plasmodium falciparum (P. falciparum) using msp-1 and msp-2 as antigenic markers.</p><p><b>METHODS</b>Parasite DNA was extracted from 100 blood samples collected from P. falciparum-positive patients confirmed by microscopy, and followed by PCR-genotyping targeting the msp-1 (block2) and msp-2 (block 3) allelic families.</p><p><b>RESULTS</b>All the families of msp-1 (K1, MAD20 and R033) and msp-2 (FC27 and 3D7) locus were observed. Results revealed that K1 (60/100) was the most predominant genotype of msp-1 allelic family followed by the genotypes of MAD20 (50/100) and R033 (45/100). In the msp-2 locus, FC27 genotype (62/100) showed higher frequency than 3D7 genotype (55/100). The allelic families were detected either alone or in combination with other families. However, no R033/MAD20 combination was observed. Multiplicity of infection (MOI) with msp-1 was higher in the locality of Ikorodu (1.50) than in Lekki (1.39). However, MOI with msp-2 was lower in the locality of Ikorodu (1.14) than in Lekki (1.76). There was no significant difference in the mean MOI between the two study areas (P=0.427).</p><p><b>CONCLUSIONS</b>The observation of limited diversity of malaria parasites may imply that the use of antigenic markers as genotyping tools for distinguishing recrudescence and re-infections with P. falciparum during drug trials is subjective.</p>

Por uma Psicologia Social não perspectivista: contribuições de Annemarie Mol / For a nonperspectivist Social Psychology: contributions of Annemarie Mol / Por una Psicología Social no perspectivista: contribuciones de Annemarie Mol

Neste artigo, buscamos discutir as contribuições da obra da filósofa holandesa Annemarie Mol para a Psicologia Social. Para isso, apresentamos algumas de suas reflexões centrais, como a abordagem não perspectivista da realidade e as noções de multiplicidade e política ontológica. Ao propor tal discussão, procuramos chamar a atenção para a possibilidade de ordenar e de coordenar a realidade de diferentes modos, bem como de reconhecer que um fenômeno é formado por múltiplos atores. Pretendemos, portanto, defender uma Psicologia Social que se aproxime das propostas da teoria ator-rede, ao buscar conexões complexas, que articulem humanos e não humanos e que façam existir múltiplas realidades. Uma Psicologia Social que fuja do realismo que caracteriza grande parte das pesquisas científicas e que não pressuponha, portanto, uma realidade anterior e independente de nossas ações, mas que tome como ponto de partida uma realidade que se torna real por meio de nossas práticas

In this article,we discuss the contributions of the work of the Dutch philosopher Annemarie Mol to Social Psychology. To do so, we present some of her main reflections, such as the nonperspectivist approach of reality and the concepts of multiplicity and ontological politics. In doing so, we aimed to call attention to the possibility of ordering and coordinating the reality in different ways and of recognizing that a phenomenon is formed by multiple actors. Therefore, we aimed to stand for a Social Psychology that follows the actor-network theory approach, as it searches complex relations, which articulate humans and nonhumans and perform multiple realities. A Social Psychology that escapes from the realism that characterizes most scientific investigations and that doesn´t presuppose a reality that is previous and independent of our actions. A Social Psychology that takes as a starting point a reality that becomes real through our practices

En este texto, buscamos discutir las contribuciones de la obra de Annemarie Mol a la Psicología Social. Para hacerlo, presentamos algunas de sus reflexiones centrales, tales como el abordaje no perspectivista de la realidad y las nociones de multiplicidad y política ontológica. Al proponer esta discusión, queremos llamar la atención para la posibilidad de ordenar y coordinar la realidad de diferentes maneras, así como también reconocer que un fenómeno está formado por múltiples y diversos actores. Buscamos, por lo tanto, defender una Psicología Social que se acerca de la Teoría del Actor-Red, al buscar conexiones complejas, que articulen humanos a no humanos y que performen diversas realidades. Una Psicología Social que huya del realismo que caracteriza gran parte de las investigaciones científicas y, por lo tanto, que no presuponga una realidad anterior e independiente de nuestras acciones, sino una realidad que se vuelva real por intermedio de nuestras prácticas

Práticas de estranhamento, indignação e resistência / Practice of questioning normality, indignation and resistance / Pratiques d'étonnement, indignation et résistance / Prácticas de extrañamiento, indignación y resistencia

O presente texto aponta algumas das importantes contribuições trazidas por Maria Helena Souza Patto para a Psicologia e a Educação em nosso país, em especial através de seu último livro, Exercícios de Indignação. Afirma-se ali não só a pesquisadora competente e cuidadora, a professora requisitada e respeitada, mas também a mulher solidária, amiga, acolhedora e indignada que indaga, estranha e coloca em análise o que vem sendo considerado como natural. São muitas e múltiplas Marias aqui apresentadas por uma de suas alunas da Pós-graduação da USP em 1988. Em especial toma relevo neste texto a prática cotidiana da resistência que os escritos de Maria Helena enfatizam. Resistência não como simples reação, mas como criação, produção de rupturas e afirmação de outras lógicas, de outras realidades. Essa é uma das múltiplas Marias aqui presentes.

The present text points out some of the important contributions made by Maria Helena Souza Patto to Psychology and Education in our country, especially through her latest book Exercises of Indignation. It is stated in her work not only the competent and caring researcher, the requested and respected professor, but also the altruistic, friendly, receiving and indignated woman, that had always questioned what was usually being considered as normal. It is possible to visualize the many "Marias" presented in this article by one of her students in USP Post-Graduation in 1988. It is especially prominent in this text the daily practice of resistance that the writings of Maria Helena emphasize. Resistance, not as mere reaction, but as creation, production of rupture, affirmation of other logics and other realities. This is one of the many "Marias" presented in this work.

Cet article souligne quelques-unes des contributions les plus importantes faites par Maria Helena Souza Patto pour la psychologie et la éducation dans notre pays, notamment à travers son dernier livre Exercícios de Indignação. Ce livre montre que l'auteur est un chercheur compétent et prudent, mais aussi une femme solidaire, indignée, sympathique et chaleureuse, qui demande, s'étonne et met à l'analyse ce qui a été considéré comme naturel. Nombreuses et multiples Marias sont présentées ici par une de ses étudiantes diplomées en l'Université de São Paulo en 1988. Ce travail met en relief la pratique quotidienne de résistance que les écrits de Maria Helena soulignent. Résistance, pas comme simple réaction, mais comme la création, la production de ruptures et l'affirmation d'autres logiques, autres réalités. Il s'agit d'un des multiples Marias ici presentées.

Este texto señala algunas de las importantes contribuciones hechas por Maria Helena de Souza Patto para la Psicología y la Educación en Brasil, en particular a través de su último libro Exercícios de Indignação. Este libro muestra no solo la investigadora competente y cuidadosa, la profesora respetada y solicitada, sino también una mujer solidaria, amiga, cálida e indignada que pregunta, extraña y pone en cuestión lo que se considera como natural. Son muchas y múltiples Marias que aquí se presenta por una de sus estudiantes de Posgrado de la Universidad de São Paulo en 1988. En este texto, se destaca que los escritos de Maria Helena insistan en la práctica de la resistencia cotidiana. La resistencia es entendida no como una mera reacción, sino como la creación, la producción de rupturas y la afirmación de otra lógica y de otras realidades. Esta es una de las muchas Marias aquí presentes.

Psicologia e liberdade: conhecimento do eu e das coisas / Psychology and freedom: knowledge of self and things

Nesse artigo, proponho investigar a significação do problema da liberdade no âmbito de um debate entre a tradição espiritualista e a psicologia experimental. Meu objetivo é o de demonstrar que a conceituação da mente como duração nãoespacializada e como multiplicidade qualitativa é capaz de suprir os empecilhos à liberdade que são instituídos pela psicologia experimental. Para tanto, recorro às obras dos filósofos Henri Bergson e Farias Brito, no tanto quanto souberam expressar e se posicionar em face dos propósitos experimentalistas. A conclusão obtida é a de que a causalidade não é determinação e, por isso, não exclui a liberdade.

In this paper, my aim is to investigate the significance of the problem of freedom in the context of the discussion between spiritualist tradition and experimental psychology. My goal is to demonstrate that the concept of mind as duration nonspatialized and as qualitative multiplicity is capable of supplying the obstacles to freedom that are established by experimental psychology. Therefore, I turn to the works of Henri Bergson and Farias Brito, in so far as recognized and positioned themselves in the face of the experimentalists' purposes. The conclusion is that causality is not determination and therefore does not exclude freedom.