Cytochrome C Oxidase Subunit 1, Internal Transcribed Spacer 1, Nicotinamide Adenine Dinucleotide Hydrogen Dehydrogenase Subunits 2 and 5 of Clonorchis sinensis Ancient DNA Retrieved from Joseon Dynasty Mummy Specimens

We analyzed Clonorchis sinensis ancient DNA (aDNA) acquired from the specimens of the Joseon mummies. The target regions were cytochrome C oxidase subunit 1 (CO1), internal transcribed spacer 1 (ITS1), nicotinamide adenine dinucleotide hydrogen (NADH) dehydrogenase subunits 2 (NAD2) and 5 (NAD5). The sequences of C. sinensis aDNA was completely or almost identical to modern C. sinensis sequences in GenBank. We also found that ITS1, NAD2 and NAD5 could be good markers for molecular diagnosis between C. sinensis and the other trematode parasite species. The current result could improve our knowledge about genetic history of C. sinensis.

Application of portable digital radiography for dental investigations of ancient Egyptian mummies during archaeological excavations: Evaluation and discussion of the advantages and limitations of different approaches and projections

PURPOSE: In the age of X-ray computed tomography (CT) and digital volume tomography (DVT), with their outstanding post-processing capabilities, indications for planar radiography for the study of the dentition of ancient Egyptian mummies may easily be overlooked. In this article, the advantages and limitations of different approaches and projections are discussed for planar oral and maxillofacial radiography using portable digital X-ray equipment during archaeological excavations. Furthermore, recommendations are provided regarding projections and sample positioning in this context. MATERIALS AND METHODS: A total of 55 specimens, including 19 skeletonized mandibles, 14 skeletonized skulls, 18 separate mummified heads, and 4 partially preserved mummies were imaged using portable digital X-ray equipment in the course of archaeological excavations led by the University of Basel in the Valley of the Kings between 2009 and 2012. Images were evaluated by 2 authors with regard to the visibility of diagnostically relevant dental structures using a 4-point grading system (Likert scale). RESULTS: Overall, the visibility of diagnostically relevant dental structures was rated highest by both authors on X-ray images acquired using a dental detector. The tube-shift technique in the lateral projections of mandibular dentition achieved the second-best rating, and lateral projections achieved the third-best rating. CONCLUSION: Conventional planar digital X-ray imaging, due to its ubiquity, remains an excellent method–and often the only practicable one–for examining the skulls and teeth of ancient Egyptian mummies under field conditions. Radiographic images of excellent diagnostic quality can be obtained, if an appropriate methodology regarding the selected projections and sample placement is followed.

Designing A Contemporary Anatomy Museum: Anatomists' Perspective.

Background: A research study was conducted in sixteen anatomy museums across India. Aim: The aim of the study is to have an integrated approach while designing a museum. Objective: The objective is to stress on the need to have a holistic approach while designing a museum so that that the museum is well planned and organised and has a huge sectional diversity that spans all aspects related to anatomy. Materials and Methods: All the museums were studied using a planned proforma that emphasised on special features of the museum with special emphasis on sectional variety. Observations: The various techniques of specimen preparation, preservation, mounting and display were observed and photographed. The sectional variety was noted. Moreover the various methods of maintaining specimen related information in pictorial and computerised catalogues was observed. Results and Conclusion: A design of a contemporary anatomymuseumcan nowbe conceived that incorporates all aspects of anatomy from history, evolution, embryology, cross-sectional anatomy, comparative anatomy, teratology, genetics and clinical anatomy to sections with modern techniques like plastination. Such amuseum will certainly have a more holistic approach to anatomy and will be more educative and scientific.

Biomphalaria alexandrina in Egypt: Past, present and future.

The African species of Biomphalaria appeared as a result of the relatively recent west-to-east trans-Atlantic dispersal of the Biomphalaria glabrata-like taxon. In Egypt, Biomphalaria alexandrina is the intermediate host for Schistosoma mansoni. Biomphalaria alexandrina originated in the area between Alexandria and Rosetta and has historically been confined to the Nile Delta. Schistosoma mansoni reached Egypt via infected slaves and baboons from the Land of Punt through migrations that occurred as early as the Vth Dynasty. The suggestion of the presence of Schistosoma mansoni infection in Lower Egypt during Pharaonic times is discussed despite the fact that that there is no evidence of such infection in Egyptian mummies. It is only recently that Biomphalaria alexandrina colonized the Egyptian Nile from the Delta to Lake Nasser. This change was likely due to the construction of huge water projects, the development of new water resources essential for land reclamation projects and the movement of refugees from the Suez Canal zone to the Delta and vice versa. The situation with respect to Biomphalaria in Egypt has become complicated in recent years by the detection of Biomphalaria glabrata and a hybrid between both species; however, follow-up studies have demonstrated the disappearance of such species within Egypt. The National Schistosoma Control Program has made great strides with respect to the eradication of schistosoma; however, there has unfortunately been a reemergence of Schistosoma mansoni resistant to praziquantel. There are numerous factors that may influence the prevalence of snails in Egypt, including the construction of water projects, the increase in reclaimed areas, global climate change and pollution. Thus, continued field studies in addition to the cooperation of several scientists are needed to obtain an accurate representation of the status of this species. In addition, the determination of the genome sequence for Biomphalaria alexandrina and the use of modern technology will allow for the study of the host–parasite relationship at a molecular level.

Paleopatología osteoarticular en Chinchorro: Revisión de un caso y discusión sobre el autocuidado en la prehistoria de Arica, norte de Chile / Osteoarticular paleopathology in a Chinchorro body: Report of one case

We report a severe osteo-arthropathy in a body of a Chinchorro adult mole exhumed from the pre-Hispanic site of Playa Miller 8, located in the city of Arica, on the northern coast of Chile. The advanced state of joint disease shows that the individual was cared by his social group, to be oble to survive. This care was perpetuated in the postmortem treatment, applying red color to the body. Particular self-care social dynamics of Chinchorro society are inferred.

Studies on protozoa in ancient remains - A Review

Paleoparasitological research has made important contributions to the understanding of parasite evolution and ecology. Although parasitic protozoa exhibit a worldwide distribution, recovering these organisms from an archaeological context is still exceptional and relies on the availability and distribution of evidence, the ecology of infectious diseases and adequate detection techniques. Here, we present a review of the findings related to protozoa in ancient remains, with an emphasis on their geographical distribution in the past and the methodologies used for their retrieval. The development of more sensitive detection methods has increased the number of identified parasitic species, promising interesting insights from research in the future.

Chagas disease in prehistory

The classical hypothesis proposes that Chagas disease has been originated in the Andean region among prehistoric people when they started domesticating animals, changing to sedentary habits, and adopting agriculture. These changes in their way of life happened nearly 6,000 years ago. However, paleoparasitological data based on molecular tools showed that Trypanosoma cruzi infection and Chagas disease were commonly found both in South and North American prehistoric populations long before that time, suggesting that Chagas disease may be as old as the human presence in the American continent. The study of the origin and dispersion of Trypanosoma cruzi infection among prehistoric human populations may help in the comprehension of the clinical and epidemiological questions on Chagas disease that still remain unanswered.

A hipótese clássica sobre a origem da doença de Chagas propõe que tenha surgido entre as populações pré-históricas dos Andes quando começaram a domesticar animais, mudaram para hábitos sedentários e adotaram a agricultura. Estas mudanças em seus hábitos de vida aconteceram há aproximadamente 6.000 anos. Entretanto, os dados da paleoparasitologia, baseados na biologia molecular, mostraram que a infecção por Trypanosoma cruzi e a doença de Chagas eram comuns tanto em populações pré-históricas da América do Sul e América do Norte muito antes deste período. De acordo com os dados paleoparasitológicos, a doença de Chagas pode ser tão antiga quanto a presença humana no continente americano. O estudo sobre a origem e dispersão da infecção por Trypanosoma cruzi entre populações humanas pré-históricas pode auxiliar na compreensão de questões clínicas e epidemiológicas sobre a doença de Chagas que ainda permanecem sem resposta.

Evaluación de la frecuencia de espina bífida oculta y su posible relación con el arsénico ambiental en una muestra prehispánica de la Quebrada de Camarones, norte de Chile / Spina bifida occulta associated with environmental arsenic exposure in a prehispanic sample from northern Chile

Background: The Camarones River Valley, located in the extreme north of Chile, is characterized by high environmental arsenic levels and an arid desert. It has been inhabited by humans for the past 7,000 years. Evidence exists for chronic arsenic poisoning in both prehispanic and present populations residing in the area. Chronic arsenic exposure causes multi-systemic problems and can induce congenital malformations, in particular neural tube development defects such as spina bifda. Aim: To study the prevalence of spina bifda among prehispanic mummies of the area. Material and Methods: Onehundred and twenty prehistoric adult individuals were analyzed for evidence of spina bifda occulta of the sacrum in skeletal samples from the sites of Camarones 8, Camarones 9, Azapa 140 and Lluta 54, held in repository at the Museo Universidad de Tarapacá de Arica- San Miguel de Azapa. A diagnosis was considered positive when at least S1, S2 or S3 were affected. As controls, mummies of individuals that resided in Lluta and Azapa valley, with a low arsenic exposure, were analyzed. Results: The frequency of spina bifda occulta among samples from the Camarones coast and Lluta and Azapa Valley were 13.5 and 2.4 percent respectively. Conclusions: Considering these were contemporaneous samples, and are believed to have had no other differences in diet or other factors, the differential exposures to arsenic could have produced the observed differences in spina bifda frequencies.

Possible influence of the ENSO phenomenon on the pathoecology of diphyllobothriasis and anisakiasis in ancient Chinchorro populations

Current clinical data show a clear relationship between the zoonosis rates of Diphyllobothrium pacificum and Anisakis caused by the El Niño Southern Oscillations (ENSO) phenomenon along the Chilean coast. These parasites are endemic to the region and have a specific habitat distribution. D. pacificum prefers the warmer waters in the northern coast, while Anisakis prefers the colder waters of Southern Chile. The ENSO phenomenon causes a drastic inversion in the seawater temperatures in this region, modifying both the cool nutrient-rich seawater and the local ecology. This causes a latitudinal shift in marine parasite distribution and prevalence, as well as drastic environmental changes. The abundance of human mummies and archaeological coastal sites in the Atacama Desert provides an excellent model to test the ENSO impact on antiquity. We review the clinical and archaeological literature debating to what extent these parasites affected the health of the Chinchorros, the earliest settlers of this region. We hypothesise the Chinchorro and their descendants were affected by this natural and cyclical ENSO phenomenon and should therefore present fluctuating rates of D. pacificum and Anisakis infestations.

Paleoparasitology of Chagas disease: a review

One hundred years since the discovery of Chagas disease associated with Trypanosoma cruzi infection, growing attention has focused on understanding the evolution in parasite-human host interaction. This interest has featured studies and results from paleoparasitology, not only the description of lesions in mummified bodies, but also the recovery of genetic material from the parasite and the possibility of analyzing such material over time. The present study reviews the evidence of Chagas disease in organic remains excavated from archeological sites and discusses two findings in greater detail, both with lesions suggestive of chagasic megacolon and confirmed by molecular biology techniques. One of these sites is located in the United States, on the border between Texas and Mexico and the other in state of Minas Gerais, in the Brazilian cerrado (savannah). Dated prior to contact with Europeans, these results confirm that Chagas disease affected prehistoric human groups in other regions outside the Andean altiplanos and other transmission areas on the Pacific Coast, previously considered the origin of T. cruzi infection in the human host.

Arseniasis and teratogenic anomalies in the atacama desert coast of ancient Chile / Arsenicismo y anomalías teratógenas en la costa del desierto de atacama en Chile prehispánico / Arsenicismo y anomalias teratógenas em populações da costa do deserto de atacama no Chile prehispánico

The model that chronic arsenic (As) exposure causes many teratogenic consequences is tested. Ancient northern Chilean populations appear to be optimal to undertake such a study as these populations are believed to have been largely stationary and ingested significant As levels over time. The Camarones River water, along the Atacama Desert, has extreme As values, 100 times above the norm. We tested the hypothesis that ancient populations, starting with the Chinchorro culture, 5000 years B.C., were significantly affected by this heavy metal and massive arsenic contamination played a role in the origin of Chinchorro artificial mummification practices. We examined 199 skeletons, housed at the Museo Arqueológico San Miguel de Azapa, Universidad de Tarapacá, Arica, Chile, searching for seven teratogenic related pathological conditions: cleft palate, polydactyly, syndactyly, spina bifida, club foot, eye malformations, and hip joint dislocation. Of the seven pathological conditions under assessment, only spina bifida was clearly found in the surveyed sample. Thus, the As teratogenic hypothesis cannot be fully demonstrated. However, the presence of spina bifida in the three valleys suggests a genetic-environmental interplay within the populations. The endemic presence of As in both the Chinchorro mummies and their material culture do strongly support the As hypothesis as a valid explanation for why the Chinchorro first began to anthropogenically mummify their dead.

En este trabajo se evalúa el modelo que la exposición crónica al arsénico (As) juega un rol importante en el desarrollo de anomalías teratógenas. Las poblaciones ancestrales del norte de Chile son adecuadas para ello, ya que por varios milenios han estado expuestas a cantidades importantes del elemento y tienen continuidad biocultural. El agua del río de Camarones, en el Desierto de Atacama, contiene niveles de As muy elevados, 100 veces lo recomendado por normas internacionales, por lo cual se testeó la hipótesis que los primeros habitantes de esta zona, los Chinchorros, estaban afectados por este metal a partir del 5000 A.C. y que el mismo influyó en el origen de complejas prácticas de momificación. Fueron examinados 199 esqueletos del Museo Arqueológico San Miguel de Azapa, Universidad de Tarapacá, Arica, Chile, buscando evidencias de siete patologías supuestamente teratógenas y relacionadas al As: labio leporino, polidactilia, sindactilia, espina bífida, pie equino, deformaciones de los ojos y dislocación de la cadera. Los hallazgos revelan que el impacto del As varía regionalmente y que las poblaciones del valle de Camarones tuvieron mayores frecuencias de lesiones a la piel y espina bífida. Los datos no avalan la hipótesis teratógena, ya que solo se encontró espina bífida en las poblaciones estudiadas. Sin embargo, la presencia de espina bífida en los tres valles muestreados implica una compleja interacción genética y medioambiental. La presencia endémica del As en las momias y en su cultura material refuerza la hipótesis del As para explicar el surgimiento de la compleja momificación artificial en poblaciones Chinchorro.

Neste trabalho foi avaliado o modelo de que a exposição crônica ao arsênico ("As") desempenha um papel importante no desenvolvimento de anomalias teratógenas. As populações ancestrais do norte do Chile são adequadas para isso, já que por vários milênios têm estado expostas a quantidades importantes do elemento e têm continuidade biocultural. A água do rio de Camarones, no Deserto de Atacama, contêm níveis de "As" muito elevados, 100 vezes o recomendado por normas internacionais, pelo qual foi testada a hipótese de que os primeiros habitantes desta zona, os Chinchorros, tinham sido afetados por este metal a partir de 5000 A.C. e que o mesmo influiu na origem de complexas práticas de mumificação. Foram examinados 199 esqueletos do Museu Arqueológico San Miguel de Azapa, Universidade de Tarapacá, Arica, Chile, procurando por evidências de sete patologias supostamente teratógenas e relacionadas ao "As": lábio leporino, polidactilia, sindactilia, espinha bífida, pé equino, deformações dos olhos e deslocamento da cadeira. Os achados revelan que o impacto do "As" varia regionalmente e que as populações do vale de Camarones tiveram maiores frequências de lessões na pele e espinha bífida. Os dados não validam a hipótese teratógena, já que somente achou-se espinha bífida nas populações estudadas. No entanto, a presença de espinha bífida nos três vales amostrados implica uma complexa interação genética e meio-ambiental. A presença endêmica do "As" nas múmias e em sua cultura material reforça a hipótese do "As" para explicar o aparecimento da complexa mumificação artificial em populações Chinchorro.

Diptera Brachycera found inside the esophagus of a mummified adult male from the early XIX century, Lisbon, Portugal

Fly puparia and adult fragments of diptera muscid were found inside the esophagus of a mummified body from the early XIX century, buried inside the crypt of the Sacrament Church (Lisbon, Portugal). The identification of the material revealed a monospecific colonization by Ophyra capensis (Wiedemann) (Diptera: Muscidae), a species known to invade corpses in the ammoniacal fermentation wave. This species can be found in corpses kept indoors, not available to the early waves of blowflies (Diptera: Calliphoridae). In the present case, the number of pupae and their developmental stage suggest that the female invaded the mummified corpse through the partially opened mouth and the oviposition took place directly inside the esophagus. This is the first case of O. capensis infesting internal organs of an intact corpse. The use of chemical products for the embalming process probably explains why external colonization did not occur.

Age Estimation of Mummies by Dental Attrition: Application of Three-dimensional CT Images

BACKGROUND: Because of the rarity of mummies in Korea and the difficulty in obtaining samples from mummies, studies to determine the ages of mummies are uncommon in Korea. This study was performed to determine the ages of mummies using the information obtained by nondestructive methods to minimize damages to the mummies. METHODS: Three mummies excavated between 2002 and 2004 were used. Three-dimensional reconstructed images of the total teeth were obtained by CT scanning. The age at death was determined according to the 'Age Estimation Table of Dental Attrition' as developed by Takei. Three teeth were extracted from each of three mummies and examined grossly and microscopically by serial sections using the Gustafson-Johanson method. RESULTS: The ages at death of the three mummies estimated by the Takei method were 23.57 years (Yoon mummy), 51.01 years (Bong mummy), and 64.45 years (Black mummy). These results were similar to the ages determined by the Gustafson method. CONCLUSION: Age determination method using a CT scan and three-dimensional reconstruction may be a valuable method because it minimizes the damages to valuable mummies and it gives reliable data similar to that obtained by other standard methods.

Paleopathologic Analysis of a Mummified Pregnant Woman of Papyung Yoon's Family

BACKGROUND: A mummy is a dead body of a human being or an animal that has been preserved artificially or naturally from decaying. Because the natural environment of Korea isn't appropriate for mummification and Korean people haven't artificially made mummies, mummies were rarely studied in Korea. METHODS: On September 6, 2002, a well-preserved female mummy was found in the grave of a family in Kyunggi-do. She was submitted to a thorough autopsy examination along with the review of genealogical documents. RESULTS: The mummy died in winter. She was pregnant and the fetal head was observed at the vaginal orifice. The uterine wall was ruptured, and the peritoneum was discolored, probably by hemorrhage. Histologically, the gastric mucosa was well preserved. On the smear cytology of gastrointestinal material and the fluid from the coffin, pollens and parasitic eggs were observed. The woman seemed to be death from hypovolemic shock due to uterine rupture during the 2nd phase of labor. CONCLUSION: From this case, we concluded the causes of the woman's mummification included the cold and dry circumstance at the time of her death, and the thick mortared wall of the grave that completely isolated the body from the outside.