ABSTRACT
PURPOSE: The purpose of this study was to examine the effects of exercise on muscle weight and Type I and II fiber cross-sectional area of affected and unaffected hindlimb muscles in rats with neuropathic pain induced by unilateral peripheral nerve injury. METHODS: Neuropathic pain was induced by ligation and cutting of the left L5 spinal nerve. Adult male Sprague-Dawley rats were randomly assigned to one of two groups: The Pain+Exercise (PE) group (n=21) and the Sham+Exercise (SE) group (n=20). All rats had 28 sessions of treadmill exercise at grade 10 for 30 minutes, twice/day at 10 m/min for 14 days. Body weight, food intake and activity were measured every day. At 15 days all rats were anesthetized and soleus, plantaris and gastrocnemius muscles were dissected. Muscle weight and Type I, II fiber cross-sectional area of the dissected muscles were measured. RESULTS: The PE group showed significant increases (p<.05), as compared to the SE group for body weight and total diet intake, muscle weight of the unaffected soleus and plantaris, and in Type I and II fiber cross-sectional area of unaffected three muscles and affected plantaris. CONCLUSION: Exercise for 14 days attenuates unaffected soleus, plantaris and gastrocnemius muscle atrophy in neuropathic pain model.
Subject(s)
Animals , Male , Rats , Body Weight , Disease Models, Animal , Eating , Hindlimb/physiology , Muscle Fibers, Skeletal/physiology , Muscular Atrophy/etiology , Neuralgia/etiology , Peripheral Nerves/injuries , Physical Conditioning, Animal , Rats, Sprague-DawleyABSTRACT
PURPOSE: The purpose of this study was to examine the effect of DHEA (Dehydroepiandrosterone) on muscle weight and Type I and II fiber cross-sectional area of affected and unaffected hindlimb muscles in rats with neuropathic pain induced by unilateral peripheral nerve injury. METHODS: Neuropathic pain was induced by ligation and cutting of the left L5 spinal nerve. Adult male Sprague-Dawley rats were randomly assigned to one of two groups: The DHEA group (n=10) had DHEA injections daily for 14 days, and the Vehicle group (n=10) had vehicle injections daily for 14 days. Withdrawal threshold, body weight, food intake and activity were measured every day. At 15 days all rats were anesthetized and soleus, plantaris and gastrocnemius muscles were dissected from the both hindlimbs. Body weight, food intake, activity, muscle weight and Type I, II fiber cross-sectional area of the dissected muscles were measured. RESULTS: The DHEA group showed significant increases (p<.05), as compared to the vehicle group for muscle weight of the unaffected plantaris, and in Type II fiber cross-sectional area of the gastrocnemius muscle. The DHEA group demonstrated a higher pain threshold than the vehicle group whereas total diet intake and activity score were not significantly different between the two groups. CONCLUSION: DHEA administration for 14 days attenuates unaffected plantaris and gastrocnemius muscle atrophy.
Subject(s)
Animals , Male , Rats , Body Weight , Dehydroepiandrosterone/administration & dosage , Disease Models, Animal , Eating/drug effects , Hindlimb , Muscle Fibers, Skeletal/drug effects , Muscle, Skeletal/drug effects , Muscular Atrophy/drug therapy , Pain/etiology , Pain Measurement , Peripheral Nerves/injuries , Rats, Sprague-DawleyABSTRACT
In this paper, effects of physical activity in daily life on m. triceps surae were considered comparing the cadavers with decubitus with those without decubitus on muscle weight and relative muscle weight. The muscles were dissected from 18 cadavers (13 males and 5 females) aged 36 to 90 which were preserved in a 10% formalin solution for about one year. Immediately after the removal, weights of gastrocnemius and soleus were measured.<BR>Mean values of the muscle weights of soleus, gastrocnemius and combined total were heavier in male than in female. Those of the relative weights of soleus were larger than those of gastrocnemius in both sexes. The muscle weights showed remarkable inter-individ-ual variations and sexual differences. The decubitus group (non-active group) showed lighter muscle weight than the normal group did in both sexes. However, there were no differences in the relative muscle weight between both groups in both sexes.<BR>The rate of lack of m. plantaris was 11.1%.<BR>From these results, it was suggested that the relative muscle weight might not be influenced by physical activity in daily life but the muscle weight might change consid-erably related to it.