ABSTRACT
In order to observe the effects of Nicotine and NNK on cultured human gingival fibroblast, several factors were examined including mutagenicity, the number of cells attached culture plate surface through MTT test, the abundance of collagen & collagenase in mRNA level and collagenolytic activity in extracellular matrix. The results were as follows; 1. Regardless of the co-existence of S9, Nicotine did not show the mutagenicity by itself and NNK by itself showd the same result; However, dose related mutagenicity was shown in NNK with S9. 2. The number of fibroblasts attached cultured plate surface was measured by MTT procedure. The number of cells in Non-smokers increased at all time periods as compared to those of smoker. 3. Non-smoker's fibroblast treated by NNK or Nicotine was dose-dependently decreased in the number of cells when compared to untreated control. In higher dose, Nicotine showed the cellular toxicity , but NNK did not. 4. No change in the abundance of mRNA for proalpha1 and proalpha2 was shown in Nicotine treated group but in gingival fibroblasts following treatment with NNK, the abundance of mRNA for proalpha1, but not proalpha2 collagen was decreased. 5. The abundance of mRNA for collagenase was decreased when NNK was treated but no change occurred in Nicotine treated group. 6. The effect of NNK and Nicotine in collagenolytic activity showed that ,collagenase activity exclusively react to type I collagen, was increased in both group, but gelatinase exclusively react to type IV collagen was not influenced at all. Collagenase activity of smoker's fibroblast was also increased as much as Nicotine and NNK group. The findings suggest that both of Nicotine and NNK lead gingival fibroblast to decrease in the abundance of collagen. And it seems to be that Nicotine and NNK have independent pathway toward the gingival fibroblast.
Subject(s)
HumansABSTRACT
Objective To study the pollution of organic components in landfill leachates which produced from industrial city-Z and their cytogenetic toxicity. Methods The landfill leachates and groundwater samples collected from an industrial city-Z were analyzed by GC-MS and determined by micronucleus test of the root-tip cells of Vicia faba. The organic extracts of the landfill leachates samples were also determined by micronucleus test of early spermatids of mice exposed to organic extracts to be tested via peritoneal injection. Results Benzene, phenol, B(a)P were found in this two samples. Significantly higher levels of micronucleus rates of the root-tip cells of Vicia faba were observed in the original landfill leachates and diluted samples(1∶10,1∶2)compared with those of the negative samples(P
ABSTRACT
ve To assess the cytogenetic toxicological safety of fish raised in water containing effluent. Methods The mutagenecity of organic extracts of uncooked meat specimens of fish raised in fishpond water mixed with sewage water taken from South and North Sewer Drainage Canals of Tianjin was detected by mice bone marrow micronucleus test. Results The organic extracts of uncooked fish meat specimens showed positive result for all of the 3 observed groups of fish raised in water of fish ponds with sewage, and negative result for fish raised in clean fishpond water in micronucleus test. Conclusion The results indicated that some kinds of organic mutagenic compounds ex-isted in the uncooked meat of fish raised in fishpond water containing effluent, which might have some potentially hazardous effects on human health through the food chains.
ABSTRACT
0.05) . Significant decreases of contents of 14 kinds of macro-elements and trace elements in purified water were found compared with those in water samples after activated carbon filtration (t = 2.496 9-5.261 7, F2 and showed significant dose-response relationship respectively (r = 0.980, P