ABSTRACT
Objective: To explore the expression of HCV core,mutation p53,and Bcl-2 proteins,and the correlation of these three proteins in the tissues of HCV infection or cirrhosis;to explore weather HCV core protein promotes the production of mutation p53 and/or the expression of Bcl-2 protein. Methods: Collect tissues from 23 HCV infected patients and determine the expression of HCV core,p53,and(Bcl-2) proteins with an immunohistochemical method(Envision method);analyze the correlation of the three proteins by statistics. Results :The positive expression of HCV core and mutation p53 proteins(primarily) lay in the nucleus,while the positive expression of Bcl-2 protein lay in the cytoplasm;the positive rate of mutation p53 was 87.0%,while the positive rate of Bcl-2 was 95.7% in the tissues with HCV core positive expression.There was no difference(P= 0.095) in expression of the three proteins;P value of correlationship test of positive intensity between HCV core and p53,HCV core and Bcl-2 was respectively 0.011 and 0.012,while the correlation coefficient was respectively 0.69 and 0.72;and P value of correlation test of positive intensity between mutation p53 and Bcl-2 was 0.007 with a correlation coefficient as 0.72. Conclusion:The expression of the three proteins is correlated: maybe the HCV core protein promotes mutation of wild p53; HCV core and mutation p53 proteins increase the expression of Bcl-2 protein.
ABSTRACT
Objective:To explore the relationship between the accelerated repopulation of tumor cells in the afterloading fractionated radiation therapy and the radiation dose,Ki67 antigen,PcNA & mutation p53 antigen expression were analyzed by continual biopsy on the same patients of cervical carcinoma two weeks (10 Gy)and four weeks(20 Gy).Methods:Ki67 antigen expression and cell cycle analysis (SPF,PI,DEN) were checked by flow cytometry,PCEA & mutation p53 antigen expression were checked by means of immunohistochemical technique and computerized image cytometry.All these indexes were viewed on their relationship with the irradiation dose.Rusults:Ki67 antigen expression and DEN were increased as radiation dose increased.The difference of Ki67,PCNA and mtP53 antigen expression at the dose of 10 Gy had statistical significance compared with before brachytherapy,but it had no statistical significance compared with at the dose of 20 Gy.Conclusion:It may be concluded that the tumor cell repopulation had occurred at the dose of 10 Gy (two weeks after brachytherapy).But these markers were not obviously changed compared with at the dose of 20 Gy,so it leads to a conclusion that tumor cell repopulation was stable.It also offers the theory basis to select the opportunity of accelerated brachytherapy of cervical carcinoma.
ABSTRACT
p53 gene mutation (exon4 , 5 , 6 . 7 . 8 and intron6) in gastric cancer and precancerous le- sions and p53 gene (exon4 and intron6) .APC gene deletion in gastric carcinomas were studied by PCR/ SSCP and PCR/RFLP. Results showed: mutation rate of p53 in intestinal metaplasia ,dysplasia and gas- tric carcinoma was 37. 5% (3/8) ,42. 1 % (8/19) , 53. 3% (16/30) ,respectively. There was significant dif- ference between groups of metaplasia、dysplasia、cancer and that of normal control. We found there were no exon8 mutation in metaplasia and dysplasia , but 4 cases in cancer group. lt is suggestted that exon8 mutation occurs at the late stage of gastric cancer , but exon 5 , 6 , 7 mutation occur in the course of pre- cancerous lesions to cancer. Loss of heterozygosity (LOH) of exon4 . intron6 . APC was 47. 4 % ( 9/19) . 8. 7%C2/23).16. 7%(3/18) ,respectively. There are some relationship between LOH of exon4 and poor- ly differentiation , lymph node metastas , depth of invasion. LOH of exon4 may be one of prognostic marker of gastric cancer. We concluded that p53 gene mutation is an early event and perhaps has syner- gism with ras oncogene in gastric carcinogenesis