HPLC Determination of Main Nucleoside and Nucleic Acid Base in Mycelia of Cordyceps / 中成药

Six nucleosides and base components in Four products of Mycelia of cultivated Cordyceps were determined. The comparison of extraction solvents and recovery test of the adenosine components were emphasized. The effective extraction-rate by water,as an extraction solvent, was higher than that by 50% alcohol. The addition of 0. 1% sodium azide into water could not only preserve putrefaction,but also make a contribution to the stability of nucleoside components in the liquid sample. When the extraction was carried out by ultrasonic at room temperature,the recovery rate of the added sample was 99. 8%.