ABSTRACT
PURPOSE: Minocycline appears to reduce the mortality rate of patients with myocardial infarction. Therefore, the cardioprotective effects of minocycline through reduction of apoptosis were studied in a rat myocardiac cell culture induced by hypoxia. METHODS: Cultured cells from the hearts of Sprague-Dawley rats were divided into two sets of groups: normoxia groups treated with 5% O2 and hypoxia groups treated with 1% O2. After several days of incubation, the control groups were not treated with minocycline, while the sample groups were treated with 1 mug/mL of minocycline. The damaged cells were observed under a microscope, while apoptosis was detected using a Tdt-mediated dUTP nick and labeling(TUNEL) assay control-stained with 4'-6'-diamidino-2-phenylindole-2HCl(DAPI). RESULTS: In normoxia, the control group showed a statistically significant difference from the sample group treated with 1 mug/mL of minocycline. In addition, in hypoxia, there was also a statistically significant difference between the two groups. When comparing the normoxia and hypoxia groups to each other, there was a statistically significant difference between the control groups and sample groups. CONCLUSION: Minocycline was found to be protective for heart in normoxia and in hypoxia in the model of rat myocardiac cell culture.
Subject(s)
Animals , Humans , Rats , Hypoxia , Apoptosis , Cell Culture Techniques , Cells, Cultured , Heart , Minocycline , Mortality , Myocardial Infarction , Rats, Sprague-DawleyABSTRACT
Objective To study the regularities of myocardiac cells with age increasing. Methods 30 male rats were used in the experiment and divided into three groups:infant group (20\|25 day), young group (3\|5 month) and old group (13\|15 month). Routine paraffine sections were made for PTAH staining and data of cell area, nuclear area, ratio of nucleus to cytoplasm, nuclear ellipticity were measured by image computer analyzer. Results With advancing age:1^Cell area and nuclear area became larger; the ratio of nucleus to cytoplasm was declined; nuclear ellipticity turned high. 2^The cell area of ventricular myocytes was bigger than that of atrial myocytes, the cell area of left atrial myocytes and left ventricular myocytes was bigger than that of right ones, and the cell area of interventricular septum was between that of right ventricular myocytes and left ones. 3^Myocardiac cells became thicker and longer; intercalated disc got distinct and complex.Conclusion The growth of nuclei seem to slower than that of myocardiac cells; the morphology of nucleus changes from round to ellipse; Round nucleus is one of important features of young cells; the cells in atrium is younger than that of ventricle.