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Objective To study the protective effects of umbilical cord stem cells on cardiac injury in severely burned rats. Methods 30 SD rats were randomly divided into sham group ,burn group and stem cells treatment group. The severely burned head ,neck and back of rat model of 35%TBSAⅢdegree was eatabished in burn and stem cells treatment groups. The blood and cardiac tissues were collected at 24 hours after stem cells transplantation. The distribution patterns of umbilical cord stem cells in cardiac tissues ,and infiltrations of neutro-phils and macrophages in cardiac tissues were detected by immunohistochemistry staining method. The cardiac structures of rats were evaluated by HE staining. The levels of Troponin I ,Creatine Kinase and Creatine Kinase-MB in serum were detected by the blood biochemical automatic analyzer. Results The umbilical cord stem cells migrated into the injured cardiac tissues and mainly concentrated in capillaries of heart. The umbilical cord stem cells significantly decreased neutrophils and macrophages infiltration of the cardiac tissues ,and markedly alleviated structure damages of the cardiac tissues in severely burned rats. Furthermore ,the levels of Troponin I ,Creatine Kinase,Creatine Kinase-MB of burn group were significantly higher than that of sham group,but those of stem cells treatment group were markedly lower than that of burn group (P < 0.05). Conclution The umbilical cord stem cells transplantation could effectively reduce inflammatory cells infiltration of the cardiac tissues,then protected the structure and function of the heart in severely burned rats.
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Objective To study the protective effects of umbilical cord stem cells on cardiac injury in severely burned rats. Methods 30 SD rats were randomly divided into sham group ,burn group and stem cells treatment group. The severely burned head ,neck and back of rat model of 35%TBSAⅢdegree was eatabished in burn and stem cells treatment groups. The blood and cardiac tissues were collected at 24 hours after stem cells transplantation. The distribution patterns of umbilical cord stem cells in cardiac tissues ,and infiltrations of neutro-phils and macrophages in cardiac tissues were detected by immunohistochemistry staining method. The cardiac structures of rats were evaluated by HE staining. The levels of Troponin I ,Creatine Kinase and Creatine Kinase-MB in serum were detected by the blood biochemical automatic analyzer. Results The umbilical cord stem cells migrated into the injured cardiac tissues and mainly concentrated in capillaries of heart. The umbilical cord stem cells significantly decreased neutrophils and macrophages infiltration of the cardiac tissues ,and markedly alleviated structure damages of the cardiac tissues in severely burned rats. Furthermore ,the levels of Troponin I ,Creatine Kinase,Creatine Kinase-MB of burn group were significantly higher than that of sham group,but those of stem cells treatment group were markedly lower than that of burn group (P < 0.05). Conclution The umbilical cord stem cells transplantation could effectively reduce inflammatory cells infiltration of the cardiac tissues,then protected the structure and function of the heart in severely burned rats.
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Objective To investigate the correlation between the cerebrocardiac syndrome (CCS) of severe type and prediction of prognosis of patients with neurosurgery .Methods Myocardial zymogram levels were measured in course of development of 120 ca‐ses of Acute Stroke(AS) that were confirmed diagnosis by brain computerized tomography (CT) .ultrasoundcardiogram(UCG)and electrocardiogram(ECG)were used to dectect Segmental Wall Motio Abnormalities (SWMA) .Results For 120 patients with AS , the specimens include 38 cases(76 .0% )of T‐wave change and 12 cases(24 .0% )of ST‐segment depression in 50 cases of ST‐T seg‐ment change ,7 cases of arrhythmia and adnormal cardiac electrical conduction .24 cases of SWM A ,32 cases(26 .7% ) of raised CK‐MB ,35 patients could be recovered to normal of ECG after active treatment .17cases of ECG improve markedly ,18 cases of ECG un‐improved ,15 cases of myocardial enzymes normally ,8 cases of myocardial enzymes improve markedly20 cases of death .Conclusion Hypertension is a risk factor of CCS ,correlation between ST‐T segment and SWMA as well as myocardial enzymes and myocardial damagein CCS was statistically analyed .
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Objective To investigate the effects of different flow volume of continuous 1:1 blood crystalloid cardioplegia on open heart surgery.Methods Retrospective randomized study was performed on 80 patients undergoing valve replacement and congenital heart disease with 1:1 blood crystalloid cardioplegia by minimum-flow continuous perfusion[0.5ml/(kg·min),group A,40 cases],high-flow continuous perfusion[(2.0 ml/(kg·min),group B,40 cases).The changes of creatine kinase(CK),isoenzyme of creatine kinase(CK-MB),lactate dehydrogenase(LDH),lactic dehydrogenase isoenzyme(LDH_1)were detected before operation and at 6,12,24,48,72 h after operation.Results The cardioplegia liquid was (41.72±7.98)ml/kg in group A,(142.09±9.65)ml/kg in group B,there was significant difference between two groups(P<0.05).During the first 72 h postoperative the value of CK,CK-MB,LDH and LDH1 in two groups were increased to different degree,serum enzymes levd increased significantly between 12,24 h after operation(P<0.05)and return to normal at 72h after operation in two groups.Butbetween two groups there was no significant difference(P＞0.05).ConclusionMinimum-flow continuous perfusion of 1:1 blood crystalloid cardioplegia appears to be superior in safety,decrease of myocardial enzymes integrity.
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180 U/L.CK-MB raised in only 5 cases(15.6%),and the highest was 28 U/L.Of the 218 MPP patients,only the 5 patients(2.3%) with raised CK-MB levels could be diagnosed as myocardial damage complicated with MPP.Conclusions The abnormal ECG is more important than myocardial zymogram in the diagnosis of myocardial damage complicated with MPP.Only higher level of certain myocardial zymogram without abnormal ECG can not be diagnosed as myocardial damage if lack of clinic and other objective evidences for myocardial damage.In watching out for myocardial damage,excessive examinations and treatments shall be avoided if possible.
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Objective To investigate the effects of local mild hypothermia therapy on ECG QT dispersion(QTd)and myocardial zymogram in patients with acute cerebral hemorrhage.Methods 70 cases of acute cerebral hemorrhage were divided into local mild hypothermia group(35 cases)and routine treatment group(35 cases)randomly.Routine treatment was used in both groups,and the local mild hypothermia therapy was just applied in local mild hypothermia group.The changes of the ECG QTd and myocardial zymogram were observed before treatment and 1 week after treatment.Results The ECG QTd was shorten and the myocardial enzymes in serum were decreased significantly in both groups at the 1 week after treatment(P
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Objective To explore the change and its significance of serum myocardial zymogram level in patients with acute cerebral hemorrhage.Methods Serum myocardial zymogram levels were detected in 82 patients with acute cerebral hemorrhage and compared with healthy controls group. The difference of serum myocardial zymogram was evaluated according to the different volume of hemorrhage. The relationship between serum myocardial zymogram level and activity of daily living (ADL) was observed. Results The level of serum myocardial zymogram in cerebral hemorrhage group was pronounced higher than those in control group (all P