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Objective: To investigate the inhibition of Shexiang Baoxin Pills (SBP) on myocardial remodeling due to the development of hepatic cirrhosis and its mechanism. Methods: To induce hepatic fibrosis, 70 rats were administered with CCl4, twice daily for eight weeks. At the end of the week 2, 10 rats were executed, the liver tissues were HE stained to confirm the formation of hepatic fibrosis, and the rest rats were randomly and equally divided into benazepril[positive control, 10 mg/(kg·d)], SBP[45 mg/(kg·d)], and model groups. The rats were ig administered for six weeks. Ten rats from the total of eighty rats were taken as the normal control group. At the end of the week 8, the blood pressure of all rats was measured, the alanine transarninase (ALT) and aspartate aminotransferase (AST) levels in serum were detected, and the collagen contents in liver and myocardial tissues were measured by Masson staining. The expression of matrix metalloproteinases (MMP-9) and tissue inhibitor of metalloproteinase (TIMP-1) were detected by Western blotting, the contents of hydroxyproline, type I and type III collagen in myocardial tissue were measured by ELISA. The liver function and hepatic fibrosis of rats in each group were observed, and the left ventricular hypertrophy index (LVHI) was analyzed. Results: There were different degrees of liver function damage, liver fibrosis, and myocardial injury in the rats in the model group. Compared with the model group, SBP could obviously reduce the contents of ALT and AST in serum and hepatic collagen (P < 0.01), the expression of myocardial MMP-9 and TIMP-1, the contents of hydroxyproline, type I and type III collagen, blue-stained collagen, and LVHI (P < 0.05). The ratio of MMP-9/TIMP-1 was obviously heightened (P < 0.05). The indexes of rats in the benazepril group were improved at different degrees. Conclusion: There is no difference in the effect of anti-hepatic fibrosis and ameliorating myocardium remodeling between SBP and benazepril. SBP has better effect on protecting liver function with damage but has no influence on blood pressure. SBP has better holistic affect on the treatment of cirrhotic cardiomyopathy than benazepril.
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@#Objective To observe the curative effects of long term statins treatment on blood lipid and left ventriclar function of myocardial infarction patients. Methods 70 patients with myocardial infarction were randomly divided into two groups: Statins treatment group (n=37) and control group (n=33). The level of blood lipid, left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), left ventricular end-diastolic volume (LVEDV), left ventricular end-systolic volume (LVESV), left ventricular ejection fraction (LVEF) were mearsured before and 2 years after the treatment. Results The long term applies of statins can bring statistically different (P<0.05) of TC and LDL-C to myocardial infarction patients. LVESD and LVEF were statistically different (P<0.05) before and after statins were used. Conclusion The long term applies of statins can improve the lipidic metabolism and left ventricular function after myocardial infarction.
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Objective To investigate the effects of tissue inhibitor-3 of matrix metalloproteinases(TIMP-3)Gene-transfected vascular smooth muscle cells(VSMCs)transplantation on heart function after acute myocardial infarction(AMI)in rats and to explore the potential mechanisms.Methods Wistar rats were produced AMI models by ligating the descending left coronary artery.Rats were survived and divided into 3 groups randomly(n=18):0.5mlDMEM containing 1?106 TIMP-3 gene-transfected VSMCs(group A),1?106 VSMCs(group B)or 0.5 ml DMEM without cell(group C)were injected into the ischemic myocardium immediately.Ischemic myocardium samples were harvested at 3 day after operation.The heart function was observed through the tissue functional examination.The activity of TIMP-3 gene-transfected VSMCs were measured by immunohistochemical method.mRNA of TIMP-3 and matrix metalloproteinase 9(MMP-9)were determined by RT-PCR.Results VSMCs were cultivated and had a high purity(98%).TIMP-3 gene was transfected into VSMCs successfully.Three day after operation in group A the average percentage of LVIDd、LVIDs、EDV and ESV were significantly higher than group normal(P
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Aim To observe the effects of GCIP-27(G protein competitive inhibitory polypeptide-27) on the structure of left ventricle in spontaneously hypertensive rats(SHR).Methods Thirty SHR rats of 12 weeks old were randomly divided into five groups(n=6):blank control groups,GCIP-27(10,30,90 ?g?kg-1,ip,bid)groups,and Losartan group(6 mg?kg-1),six Wistar-Kyotos(WKY) rats were as normal control.Systolic blood pressure(SBP)was measured once per two weeks with tail-sleeve methods.At the end of the 8th week,left ventricle weight index(LVWI)were determined.Myocardium structure was observed by polaroscope and transmission electron microscope.The area and content of myocardial interstitial collagen were treated for semiquantitative analysis by image analyzer system.Results The SBP in GCIP-27(10,30,90 ?g?kg-1)groups decreased significantly from the 2 nd to the 8th week by comparison with blank control group.GCIP-27(10,30,90 ?g?kg-1) lowered heart mass index(HMI)and left ventricular mass index(LVMI)markedly compared with blank control group(P