ABSTRACT
Lippia sidoides Cham is a typical herb species of Northeast Brazil with widespread use in folk medicine. The major constituents of the essential oil of L. sidoides (EOLs) are thymol, p-cymene, myrcene, and caryophyllene. Several studies have shown that the EOLs and its constituents have pharmacological effects, including antibacterial, anti-inflammatory, antioxidant and neuroprotective activity. Therefore, this work aimed to investigate the effects of the EOLs and their main constituents on rat sciatic nerve excitability. The sciatic nerves of adult Wistar rats were dissected and mounted in a moist chamber. Nerves were stimulated by square wave pulses, with an amplitude of 40 V, duration of 100 μs to 0.2 Hz. Both EOLs and thymol inhibited compound action potential (CAP) in a concentration-dependent manner. Half maximal inhibitory concentration for CAP peak-to-peak amplitude blockade were 67.85 and 40 µg/mL for EOLs and thymol, respectively. CAP peak-to-peak amplitude was significantly reduced by concentrations ≥60 µg/mL for EOLs and ≥30 µg/mL for thymol. EOLs and thymol in the concentration of 60 µg/mL significantly increased chronaxie and rheobase. The conduction velocities of 1st and 2nd CAP components were also concentration-dependently reduced by EOLs and thymol in the range of 30-100 µg/mL. Differently from EOLs and thymol, p-cymene, myrcene and caryophyllene did not reduce CAP in the higher concentrations of 10 mM. These data demonstrated that EOLs and thymol inhibited neuronal excitability and were promising agents for the development of new drugs for therapeutic use.
Subject(s)
Animals , Male , Female , Alkenes/pharmacology , Lippia/chemistry , Monoterpenes/pharmacology , Oils, Volatile/pharmacology , Sciatic Nerve/drug effects , Sesquiterpenes/pharmacology , Thymol/pharmacology , Action Potentials/drug effects , Action Potentials/physiology , Nerve Block/methods , Neural Conduction/drug effects , Oils, Volatile/chemistry , Rats, Wistar , Reproducibility of Results , Sciatic Nerve/physiology , Time FactorsABSTRACT
Pimenta racemosa var. racemosa (Mill.) J.W. Moore, is a Caribbean arboreal plant belonging to the family Myrtaceae with a special interest in their leaves for the production of essential oil, commonly known as "Bay-Rum". Steam distillation of fresh leaves collected in April 2012 produced two types of essential oil, one of these resulted lighter than water and was named (AL) and the other one heavier than water (AP). Gas chromatography/mass spectrometry analysis showed the presence of 17 components constituting 99.9 percent for AL, being the major compounds: eugenol (60.4 percent), myrcene (11.7 percent), chavicol (6.0 percent), limonene (5.4 percent) and linalool (4.4 percent); for the AP, 13components were identified (98.3 percent) from which eugenol (82.9 percent) and chavicol (9.3 percent) were in major proportions.
Pimenta racemosa var. racemosa (Mill.) J.W. Moore, es una planta caribeña, arbórea, perteneciente a la familia Myrtaceae y con un especial interés en sus hojas para la producción del aceite esencial comúnmente conocido como Bay-Rum. La destilación por arrastrecon vapor de las hojas frescas colectadas en abril 2012 produjo dos tipos de aceites esenciales, uno ligero (AL) y otro más pesado que elagua (AP). El análisis de sus componentes volátiles por cromatografía de gases/espectrometría de masas mostró 17 componentes lo que constituye el 99,9 por ciento del AL de los cuales los mayoritarios fueron eugenol (60,4 por ciento), mirceno (11,7 por ciento), chavicol (6,0 por ciento), limoneno (5,4 por ciento) ylinalool (4,4 por ciento); para el AP, 13 componentes fueron identificados (98,3 por ciento) de los cuales eugenol (82,9 por ciento) y chavicol (9,3 por ciento) fueron los mayoritarios.
Subject(s)
Oils, Volatile/chemistry , Plant Leaves/chemistry , Pimenta/chemistry , Anisoles/analysis , Eugenol/analysis , Gas Chromatography-Mass Spectrometry , Monoterpenes/analysis , Terpenes/analysis , VenezuelaABSTRACT
Essential oil from leaves of Ageratina jahnii (B.L.Rob.) R. M. King & H. Rob. and Ageratina pichinchensis (Kunth) R. M. King & H. Rob (Asteraceae) collected in January 2010 were analyzed by GC/MS. Oils extracted by hydrodistillation yielded 0.50 percent and 0.43 percent w/v, respectively. Fifteen and twenty five components were identified by comparison of their mass spectra with the Wiley GC-MS Library data and by their retention indices (RI). The major components identified in A. jahnii were beta-myrcene (37.6 percent, alpha-pinene (17.1 percent), limonene (8.8 percent and pentacosane (9.2 percent while for A. pichinchensis 8,9-epoxythymyl isobutyrate (20.2 percent, germacrene-D (19.8 percent, thymyl isobutyrate (10.8 percent, eupatoriochromene (6.5 percent) and encecalol (5.9 percent) were observed as main compounds. This is the first report regarding the essential oil composition and antibacterial activity of the essential oil of A. jahnii.
Aceites esenciales de las hojas de Ageratina jahnii (B.L.Rob.) R. M. King & H. Rob. y Ageratina pichinchensis (Kunth) R. M. King & H. Rob (Asteraceae) colectadas en enero 2010 fueron analizados por CG/EM. Los aceites extraídos por hidrodestilación produjeron 0,50 por ciento y 0,43 por ciento p/v de rendimiento, respectivamente. Quince y veinticinco compuestos fueron identificados por comparación de sus espectros de masas con la base de datos de la librería Wiley CG/EM y por sus índices de retención (IR). Los compuestos identificados como majoritarios en A. jahnii fueron beta-mirceno (37,6 por ciento), alfa-pineno (17,1 por ciento, limoneno (8.8 por ciento y pentacosano (9,2 por ciento mientras para A. pichinchensis isobutirato de 8,9-epoxitimilo (20,2 por ciento, germacreno-D (19,8 por ciento, isobutirato de timilo (10,8 por ciento), eupatoriocromeno (6,5 por ciento y encecalol (5,9 por ciento) fueron observados como compuestos mayoritarios. Este es el primer reporte sobre la composición química y actividad antibacteriana del aceite esencial de A. jahnii.
Subject(s)
Oils, Volatile/pharmacology , Oils, Volatile/chemistry , Ageratina/chemistry , Anti-Bacterial Agents/pharmacology , Plant Extracts/pharmacology , Anti-Bacterial Agents/chemistry , Bacteria , Gas Chromatography-Mass Spectrometry , Microbial Sensitivity Tests , Monoterpenes/analysis , Sesquiterpenes/analysis , VenezuelaABSTRACT
The objective of this study was to investigate the effect of vermicompost, worm-bed leachate (WBL) and Glomus mosseae, an arbuscular mycorrhizal fungi (AMF), on growth of lemongrass (Cymbopogon citratus (DC.) Stapf). A response surface methodology, i.e. a three-level Box Benhen design with three repetitions and three blocks, was applied to optimize biomass production, essential oil yield and its composition. Application rates of Glomus mosseae were 0, 1 or 2 g plant-1, vermicompost 0, 5 or 10 g plant-1 and WBL 0, 10 and 20 percent. The AMF had no significant effect on the variables tested, but vermicompost had a significant effect on essential oil yield and WBL on essential oil yield, myercene concentration and shoot dry weigh (p < 0.05). It was found that lemongrass fertilized with 2.0 g G. mosseae, 5.0 g vermicompost and 20 percent worm-bed leachate would yield 0.797 percent essential oil of which 62.6 percent was citral.
Subject(s)
Oils, Volatile/chemistry , Composting , Cymbopogon/chemistry , Fungi/physiology , Mycorrhizae/physiology , Soil , Culture Media , Process OptimizationABSTRACT
INTRODUCCIÓN: es necesario definir la época adecuada de plantación para cultivar exitosamente Tagetes lucida. OBJETIVOS: obtener valores altos de biomasa y aceite esencial. MÉTODOS: se establecieron estaquilleros y posterior trasplante en 2008 (30 de abril-27 de mayo; 27 de mayo-8 de julio; 24 de junio-30 de julio; 5 de agosto-11 de septiembre). Se determinó la supervivencia de estacas enraizadas en cultivo a pleno sol, la altura de las plantas en el momento de cosecha y el rendimiento fresco de follaje en 4 cosechas; generalmente, a 3 meses del trasplante y las restantes a intervalos de 3 meses. Se estimaron los contenidos de aceite en las cosechas segunda y tercera mediante hidrodestilación y la presencia de componentes por cromatografía en capa delgada (referencias: eugenol, linalol, mirceno). RESULTADOS: se obtuvo supervivencia 98 por ciento en el trasplante; la mayoría de las plantas no superaron 60 cm de altura, se determinó que en el período octubre-inicios de febrero las plantas presentaron completa floración, en mayo floración escasa y en junio-septiembre estado vegetativo y su crecimiento se detiene en invierno. Se demostró que en un período de explotación de 12-13 meses, se alcanzaron los mayores valores en la segunda fecha de plantación, que en la mayoría de las cosechas, salvo en la cuarta, presentaron abundante follaje en floración con altos porcentajes de aceite esencial y que se produce incremento significativo desde la primera hasta la cuarta cosecha, influenciada por las precipitaciones del período lluvioso (mayo/agosto-septiembre/2009). Se constató que después de 6 cortes del follaje, las plantas mostraron formación de tocones de tallos viejos que proporcionan mucho tallo lignificado y, consecuentemente, disminución de calidad del material vegetal a cosechar.
INTRODUCTION: it is necessary to define the appropriate planting period to cultivate successfully Tagetes lucida. OBJECTIVES: to obtain high values of biomass and essential oil. METHODS: picket fences were placed and posterior transplant in 2008 (April 30-May 27-July 8, June 24-30, and August 5-September 11). The survival of rooted stakes was determined in broad daylight culture, the plant's height at harvest and the fresh yield of foliage in four harvests; generally, at three months from the transplant and the remainder ones at three months intervals. The oil content in second and third harvests was estimated by hydrodistillation and presence of components by thin layer chromatography (references: eugenol, linalool, myrcene). RESULTS: there was a 90 percent survival in transplant; most of plants not passed of 60 cm height, determining that in October-the beginning of period February the plants showed a total flowering, in May a scarce flowering and in June-September a vegetative state and their growth stop in winter. It was demonstrate that a farming period of 12-13 months, the higher values in the second planting date were achieved than in most of harvests, except in the fourth one, where they had a abundant foliage in flowering with high percentages of essential oil and a significant increase from the first one up to the fourth harvest, influenced by rainfalls (May/August-September/2009). We confirmed that after the 6 foliage cuts, plants showed the stump of old stems with many lignin stems and consequently, a decrease in the plant material quality to be grown. CONCLUSIONS: the better planting date was where the picket fence was established was May 27, the harvests when plants has flowering and in culture must to be eliminated after 1,5 years