ABSTRACT
myrsine coriacea (Sw.) R. Br. ex Roem. & Schult. (Primulaceae) conhecida popularmente como capororoquinha ou capororoca, é amplamente distribuída nas regiões sul e sudeste do Brasil. As espécies desse gênero apresentam um potencial antioxidante e anti-inflamatório, que pode ser acessado na busca de novos ativos para o tratamento de desordens pigmentares da pele. Desta forma, este trabalho teve como objetivos avaliar o potencial antitirosinase e antioxidante de extratos e frações de M. coriacea e identificar os possíveis compostos responsáveis por essas atividades. Foram realizados ensaios para avaliar o potencial antioxidante das amostras através do método do DPPH, enquanto a capacidade hipopigmentante das amostras foi avaliado pela inibição da enzima tirosinase. Como complemento, foram determinados os teores de compostos fenólicos totais e flavonoides através dos métodos colorimétricos empregando o reagente Folin-Ciocalteau e AlCl3. Adicionalmente, os extratos de M. coriacea tiveram avaliados seus potenciais citotóxicos utilizando diferentes linhagens tumorais humanas. O perfil fitoquímico de M. coriacea foi analisado por cromatografia a gás acoplada com espectrometria de massas (CG-EM) e cromatografia em camada delgada (CCD) com padrões. Nessas análises foram identificados 34 compostos, sendo o ácido palmítico e o palmitato de etila os compostos majoritários nas amostras de M. coriacea. O extrato bruto das folhas apresentou o maior teor de fenólicos totais, enquanto a fração de acetato de etila das folhas teve o maior teor de flavonoides. Contudo, o extrato bruto dos frutos apresentou a melhor atividade antioxidante de todas as amostras analisadas, apresentando também a melhor atividade antitirosinase. Dentre os compostos anotados, mandenol, ácido -linoleico e o linolenato de etila foram os compostos considerados como possíveis inibidores da tirosinase, com boa interação molecular com a enzima nas análises de ancoragem molecular in silico. Das amostras analisadas com relação a inibição de crescimento frente as células tumorais, a amostra da fração de clorofórmio das folhas foi a que apresentou potencial antitumoral frente as células de adenocarcinoma de cólon (HCT116)
myrsine coriacea (Sw.) R. Br. ex Roem. & Schult. (Primulaceae) popularly known as capororoquinha or capororoca, is widely distributed in southern and southeastern Brazil. Myrsine species have an antioxidant and anti-inflammatory potential, which can be accessed in the search for new actives for the treatment of skin pigmentation disorders. Thus, this work aimed to evaluate the antityrosinase and antioxidant potential from extracts and fractions of M. coriacea and to identify the probable compounds responsible for these activities. Assays were performed to evaluate the antioxidant potential of the samples using the DPPH method, while the hypopigmentation capacity of the samples was evaluated by the tyrosinase inhibition. As a complement, the amounts of total phenolic compounds and flavonoids were determined through colorimetric methods using the Folin-Ciocalteau reagent and AlCl3. Additionally, M. coriacea extracts had their cytotoxic potential evaluated using different human tumor cell lines. M. coriacea phytochemical profile was obtained by gas chromatography coupled with mass spectrometry (GC-MS) and thin layer chromatography (TLC) with standards. In these analyses, 34 compounds were identified, with palmitic acid and ethyl palmitate as the major compounds in M. coriacea samples. The leaf crude extract presented the highest total phenolics contents, while the leaf ethyl acetate fraction had the highest flavonoid amounts. However, the fruit crude extract showed the best antioxidant and antityrosinase activities of all analyzed samples. Among the annotated compounds, mandenol, -linoleic acid and ethyl linolenate were the compounds considered as putative tyrosinase inhibitors, presenting good molecular interaction with the enzyme active site in the in silico molecular docking analysis. The leaf chloroform fraction was the only sample that showed an antitumor potential against colon adenocarcinoma cells (HCT116)
Subject(s)
Monophenol Monooxygenase/analysis , Primulaceae/metabolism , Myrsine/classification , Fruit/classification , Antioxidants/analysis , Mass Spectrometry/methods , Skin Pigmentation/immunology , Chromatography, Thin Layer/methods , Hypopigmentation/pathologyABSTRACT
ABSTRACTThe antimicrobial activity of the myrsinoic acid A isolated from Myrsine coriacea (Sw.) R.Br. ex Roem. & Schult., Primulaceae, and a two semi-synthetics derivatives was tested against Bacillus subtilis, Escherichia coli, Salmonella enterica subsp. enterica serovar typhi, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Micrococcus luteus, Candida albicans, Candida krusei and Candida tropicalis. The microdilution method was used for the determination of the minimum inhibitory concentration during evaluation of the antimicrobial activity. The myrsinoic acid A showed no activity against the selected microorganisms but the hydrogenated and acetylated derivatives were active against B. subtilis, E. coli, S. aureus and P. aeruginosa.
ABSTRACT
Objective: To study the chemical constituents from the radix and rhizome of Myrsine stolonifera. Methods: The chemical constituents were isolated and purified by D101 macroporous adsorption resin, silica gel, and Sephadex LH-20 column chromatography. The structures of the isolated compounds were identified on the basis of physicochemical properties and spectroscopic methods including 1H-NMR, 13C-NMR, and MS spectra. Results: Eleven compounds were identified as askaempferol (1), dihydrokaempferol (2), quercetin (3), 5, 7, 4'-trihydroxyisoflavone (4), chrysoeriol (5), quercetin-7-O-α-D-glucoside (6), (-)- epicatechin (7), 3, 5-dimethoxy-benzylalcohol-4-O-β-D-glucopyranoside (8), 2, 6-dimethoxy-4-hydroxyphenol-1-O-β-D- glucopyranoside (9), syringing (10), and (+)-lyoniresinol3α-O-β-D-glucopyranoside (11). Conclusion: Compounds 2, 4-11 are all isolated from the plants of Myrsine Linn. for the first time, and compounds 1-11 are all isolated from this plant for the first time.
ABSTRACT
Birds are known to be important dispersers of plants, since the passage of seeds through the digestive tract often has a positive influence on germination percentage and speed. In this study, proportions of germination in different substrates were compared between Myrsine (Myrsinaceae) seeds collected manually and those ingested by birds. The guilds of birds that fed on M. umbellata and M. lancifolia were identified and comprise 24 and 12 species, respectively. A higher germination proportion was found among seeds defecated by birds and sowed in the study area. The study also found differences between the guilds of frugivores in both synchronopatric species. Therefore, we suggest that M. umbellata and M. lancifolia are important food sources by birds that play a positive influence for enhance germination of Myrsine.
As aves são reconhecidamente importantes agentes dispersores de plantas, uma vez que a passagem da semente pelo trato digestório muitas vezes interfere positivamente na porcentagem e velocidade de germinação de sementes. No presente estudo, as porcentagens de germinação, em diferentes substratos, foram comparadas entre sementes ingeridas por aves e sementes não-ingeridas (coletadas manualmente) de Myrsine (Myrsinaceae). A guilda de aves que se alimentou de M. umbellata e M. lancifolia foi identificada com 24 e 12 espécies, respectivamente. Verificou-se maior porcentagem de germinação nas sementes defecadas por aves, que foram semeadas em solo da área de estudo. Além disso, constataram-se diferenças nas guildas de frugívoros entre as duas espécies sincronopátricas. Portanto, nós sugerimos que M. umbellata e M. lancifolia são importantes fontes alimentares para aves que têm uma influência positiva para o aumento na germinação de Myrsine.