ABSTRACT
Objective: To study the chemical constituents from the fruits of Tribulus terrester. Methods: The constituents were isolated and purified by silica gel column chromatography, Sephadex LH-20 column chromatography, preparative HPLC and TLC methods. The structures were identified by HR-ESI-MS and spectral analysis methods. Results: Fourteen compounds were isolated from the fruits of Tribulus terrester and the structures were identified as N-p-feruloyltyramine (1), N-trans-p-coumaroyl-3-O- methyldopamine (2), methyl ferulic acid (3), caffeic acid methyl ester (4), protocatechuic acid methyl ester (5), trifilines A (6), 5β-spirost-25(27)-en-3β-ol-12-one 3-O-{β-D-glucopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→3)]-β-D-glucopyranoside} (7), tribulosaponin B (8), isoterrestrosin B (9), 25(R)-spirostane-3,5-diene-12-one (10), 25 (R)-spirostane-24β-ol-4-ene-3,12-dione (11), 26-O-β-D-glucopyranosyl-(25S)-5α-furostane-20 (22)-en-12-one-3β,26-diol-3-O-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl- (1→4)]-β-D-galactopyranoside (12), terrestrinone A1 (13), and terrestrinone A2 (14). Conclusion: Compounds 1-7 are obtained from the Zygophllyaceae for the first time.
ABSTRACT
Objective: To investigate the chemical constituents in the dried leaves of Sabia fasciculata. Methods: Column chromatography such as silica gel, Sephadex LH-20, and preparative HPLC were used to isolate and purify the compounds. Spectroscopic methods like MS, 1H-NMR and 13C-NMR, and physical constants were used to elucidate their structures. Results: Fifteen compounds were isolated from 95% ethanol extracts of S. fasciculata, including seven pentacyclic triterpenoids, such as methyl-3-oxo-olean-12-ene-28-oate (1), betulin (2), 3-oxo-olean-Δ11,13(18)-diene (3), oleanolic acid (4), imberic acid (5), pseudo- ginsenoside RP1 (6), and chikusetsusaponin IVa (7); three flavonoids, such as quercetin (8), rutin (9), and mutabiloside (10); three alkaloids, such as fuseine (11), N-p-feruloyltyramine (12), and N-trans-coumaroyl tyramine (13); two steroids, such as β-sitosterol (14) and β-daucosterol (15). Conclusion: All the compounds are isolated from the plant for the first time. Compounds 1, 5-7, and 10 are isolated from the plants of Sabia Colelbr. for the first time.