ABSTRACT
A 45 year old female patient complaining of pain, Sparsha Asahatwa (tenderness), Shotha (inflammation) crackle sounds of the joints and warmth for 4 years but severe since 5 months and restricted movement of bilateral knee join, was being diagnosed as a case of Janu Sandhigata Vata (Osteoarthritis of knee joint) treatment planned with Hingwashtaka Choorna, Maha Yograj Guggulu, Maha Rasnadi Kadha, Gandharva Haritaki Choorna ausadhi and Bahiparimarjana Chikitsa i.e., Naga Mircha Lepa. There was a significant improvement in the scale of pain, range of movement and standing time along with diminution of inflammation and tenderness after treatment. Patient responded to the Ayurvedic treatment. After the therapy patient felt improvement.
ABSTRACT
In the present research paper an attempt has been made to introduce standard operating procedure (SOP) for preparation of Naga Bhasma described in Ayurvedic formulary of India (AFI) using traditional Puta method. A total 60 Putas were given, out which first 50 Puta were Ardha gajaputa, followed by last 10 Puta as Gajaputa. In this method Manahshila and Kanji were taken as media. Arddha Gaja Puta (burnt with 4 kg Cow dung cake) was given for first 50 puta and the last ten Putas was given Gajaputa (burnt with 8 kg cow dung cake). The percentage increase in the Naga bhasma prepared after 60 Puta was 12%. Upto 50 Puta the increase in weight of Naga was 208%, while in last 10 Putas the percentage of decrease in weight was 63.66%. The average percentage purity of Naga decreased from 93.18% to 81.44% after Shodhana. The percentage of (Pb) Lead in Naga Bhasma 50 Puta and 60 Puta were 14.118% and 14.872 % respectively. Chemically Naga Bhasma was found in the form of PbS form.
ABSTRACT
The complete coding sequences of three sheep genes- BCKDHA, NAGA and HEXA were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR), based on the conserved sequence information of the mouse or other mammals. The nucleotide sequences of these three genes revealed that the sheep BCKDHA gene encodes a protein of 313 amino acids which has high homology with the BCKDHA gene that encodes a protein of 447 amino acids that has high homology with the Branched chain keto acid dehydrogenase El, alpha polypeptide (BCKDHA) of five species chimpanzee (93 percent), human (96 percent), crab-eating macaque (93 percent), bovine (98 percent) and mouse (91 percent). The sheep NAGA gene encodes a protein of 411 amino acids that has high homology with the alpha-N-acetylgalactosaminidase (NAGA) of five species human (85 percent), bovine (94 percent), mouse (91 percent), rat (83 percent) and chicken (74 percent). The sheep HEXA gene encodes a protein of 529 amino acids that has high homology with the hexosaminidase A(HEXA) of five species bovine (98 percent), human (84 percent), Bornean orangután (84 percent), rat (80 percent) and mouse (81 percent). Finally these three novel sheep genes were assigned to GenelDs: 100145857, 100145858 and 100145856. The phylogenetic tree analysis revealed that the sheep BCKDHA, NAGA, and HEXA all have closer genetic relationships to the BCKDHA, NAGA, and HEXA of bovine. Tissue expression profile analysis was also carried out and results revealed that sheep BCKDHA, NAGA and HEXA genes were differentially expressed in tissues including muscle, heart, liver, fat, kidney, lung, small and large intestine. Our experiment is the first to establish the primary foundation for further research on these three sheep genes.