ABSTRACT
Objective To investigate NIMA-related kinase 2 (NEK2) expression in colorectal cancer (CRC) tissues and its effect on the proliferation, migration and invasion of HCT116 cells. Methods Synthesized siRNA targeting NEK2 was transfected into HCT116 cells by lipofectamine method. CCK8 assay, FACS, wound healing assay and Transwell assay were used to detect the effects of NEK2 knockdown on cell proliferation, cell cycle distribution, migration and invasion. Western blot was carried out to detect the expression of E-cadherin, N-cadherin, CDK4 and cyclin D1. Results The expression of NEK2 in CRC tissues was significantly higher than that in adjacent normal tissues (65.0% vs. 35.0%, χ2=14.593, P < 0.01), and its level was closely related to TNM stage, lymph node metastasis and distant metastasis (all P < 0.05). NEK2 protein and mRNA levels in CRC cell lines were also significantly higher than those in normal colorectal mucosal cells (P < 0.01). After NEK2 was knocked down by siRNA, HCT116 cells were arrested in G0/G1 cycle, while cell proliferation, invasion and migration were significantly reduced (P < 0.01). NEK2 interference in HCT-116 cells led to the upregulation of E-cadherin and downregulation of N-cadherin, CDK4 and cyclin D1 at protein levels. Conclusion The high NEK2 expression is correlated with clinicopathological characteristics of CRC patients. NEK2 knockdown by siRNA could effectively inhibit the proliferation, invasion and migration abilities of colorectal cancer cells, suggesting that NEK2 plays an important role in the occurrence and development of colorectal cancer and it can be used as a potential treatment target.
ABSTRACT
42 cases of OSCC and 20 of healthy tissues were studied to detect the expression of NIMA-related Kinase 2(NEK2)mRNA by Real-time PCR.Over expression of NEK2 mRNA was observed in OSCC (z =-6.328,P 0.05),age (z =-0.365,P >0.05)and gender (z =-3.450,P >0.05).NEK2 gene may be involved in the develop-ment of OSCC.
ABSTRACT
Purpose To detect the expression of Nek2, Plk1 and Cdk1 in gastric carcinoma and to explore their correlation with clini-copathological factors. Methods We used immunohistochemistry to detect the protein expression of Nek2, Plk1 and Cdk1 in 80 cases of gastric carcinoma. Results The positive rates of Nek2, Plk1 and Cdk1 expressions in 80 cases of gastric carcinoma were 64%, 79% and 85% respectively. While the positive rates of Nek2, Plk1 and Cdk1 expressions in 20 cases normal gastric tissue were 15%, 10% and 20% respectively,with statistical significance (P0. 05). The expression of Plk1 and Cdk1 had a correlation with tumor size, differentiation of gastric cancer, invasion depth, lymph node metastasis and TNM stage (P0. 05). There were significant associations between Nek2 and Plk1, Plk1 and Cdk1, Nek2 and Cdk1 expression (P <0. 01). Conclusions Abnormal expression of Nek2, Plk1 and Cdk1 might be one of the mechanisms of tumorigenesis, especially of abnormal tumour proliferation. They may represent new potential targets for therapeutic intervention.