ABSTRACT
Objective To compare and analyze the genetic structure of NIH mice bred in Unites A and B, using microsatellite technology.Methods Thirty SPF 8-week old outbred NIH mice (half male and half female) of each population were randomly chosen from the Units A and B, respectively.PCR amplification and STR scan were performed to determine the genetic characteristics of two outbred populations using microsatellite loci, and the population genetic structure was analyzed with statistical software Popgene 1.32.Results In the NIH mouse population form the Unit A, 74 alleles were obtained, with an average heterozygosity of 0.3108 and polymorphism information content of 0.2637.In the NIH mouse population from the Unit B, 76 alleles were obtained, with an average heterozygosity of 0.3257 and polymorphism information content of 0.2777.The inter-population comparison showed that genetic differentiation coefficient Fst was 0.3932, the genetic identity was 0.3971, and the genetic distance was 0.9235.The population difference was significant.Conclusions There is serious genetic differentiation between the two NIH mice populations,resulting in the formation of two different closed populations.
ABSTRACT
The aim of the present study was to evaluate the effect of adult Fasciola hepatica excretion-secretion products administered intraperitoneally (ip) to NIH mice, on the number of cells producing antibodies against sheep erythrocytes (thymus dependent antigen). Thirteen mice were distributed at random into two groups which were treated ip, daily during seven days, with either phosphate buffered saline (Group I: PBS, n = 6) or excretion-secretion products of adult Fasciola hepatica (Group II: ESP_Fh, n = 7). At day eight, each mouse in both groups was immunized ip with sheep erythrocytes and four days later, the number of plaque-forming cells (PFC) in a suspension of spleen cells per each mouse was recorded. The mean number (± SEM) of PFC obtained in the group PBS was significantly higher (72 ± 10; P < 0.01) than the value observed in the group ESP_Fh (27 ± 9). The results indicate that ESP_Fh induce immunosupression to thymus-dependent antigens in mice.
El objetivo del presente estudio fue evaluar el efecto de los productos de excreción-secreción de Fasciola hepatica adulta administrados vía intraperitoneal (ip) a ratones NIH, sobre el número de células productoras de anticuerpos contra eritrocitos de borregos (antígeno timodependiente). Trece ratones fueron distribuidos al azar en dos grupos que fueron tratados ip, diariamente durante siete días, ya sea con solución salina amortiguadora de fosfatos (Grupo I: SSAF, n = 6) o con productos de excreción-secreción de Fasciola hepatica adulta (Grupo II: PES_Fh, n = 7). Al día ocho, cada ratón en ambos grupos fue inmunizado vía ip con eritrocitos de borrego y cuatro días más tarde se determinó el número de células formadoras de placa (CFP) en una suspensión de células del bazo de cada ratón. El número promedio (± EEM) de CFP obtenido en el grupo SSAF fue significativamente más alto (72 ± 10; P < 0.01) que el valor observado en el grupo PES_Fh (27 ± 9). Los resultados indican que los PES_Fh inducen inmunosupresión a antígenos timodependientes en ratones.
ABSTRACT
Objective To study the protective effects of aqueous extract and alcohol-soluble extract of Isodon lophanthoides var.gerardianus (Benth.)Hara (ILVG) on immunity hepatic injury induced by concanavalin A (Con A) in mice.Methods One hundred and eight NIH mice were allocated into normal control group,model group,ILVG groups of aqueous extract and alcohol-soluble extract (low-,middle-and high-dosage),and bifendate group randomly.In the experimental groups,mice received either ILVG aqueous extract or alcohol-soluble extract (18.20 g?kg-1,9.10 g?kg-1,4.55 g?kg-1 respectively) or Bifendate (45 mg?kg-1) by gastric perfusion daily for consecutive 5 days.In the 5th day,Con A (20 mg?kg-1) was injected into mice via the tail vein 4h after administration.And then the blood was obtained by picking out the eyeball and the serum was separated after 8-hour fasting.The serum levels of ALT and AST were analyzed,the body weight as well as the weight of liver,spleen and thymus were measured,and pathological features of hepatic tissue were observed.Results ILVG can decrease the ALT and AST,restrain the enlargement of liver and the shrinkage of thymus and reduce the necrosis of hepatic tissue.Conclusion Aqueous extract and alcohol-soluble extract of ILVG possess the effects of protecting liver from immunity injury induced by Con A in NIH mice.