ABSTRACT
Abstract Objectives We aimed to explore the heterogeneity and differentiation trajectories of epithelial cells and NK/T-cells in Laryngeal Squamous Cell Carcinoma (LSCC). Methods We downloaded the GSE150321 data set containing LSCC01 and LSCC02 samples single cell RNA data from Gene Expression Omnibus. The UMAP analysis was performed to identify the cell subpopulations and cell locations of subpopulations. Seurat package was used to analyze the differential expression of genes. The function of differential expression genes was analyzed using DAVID database. The monocle2 package was used to analyze differentiation trajectories. We used the CellChat package to observe the signaling pathways and ligand-receptor pairs for epithelial cells and NK/T-cells. Results All the LSCC cells were divided into 16 subpopulation that included 7 epithelial cell subsets, 3 T-cell subsets. The function analysis indicated that epithelial cells and NK/T-cells mainly participated in different process, such as cell cycle, immune response, and cell migration. Then, the results of differentiation trajectory indicated that the ability of migration, and the activation of the immune system increases, while the ability of apoptosis, and glucose metabolic process decreases as pseudotime. Migration-related epithelial cells act on all T-cells via the CNTN2-CNTN2 ligand-receptor pair, which suggested that CNTN2 might be an important biomarker for regulating migration of epithelial cells. Conclusions Our study characterized the heterogeneity of LSCC, which provided novel insights into LSCC and identified a new mechanism and target for clinical LSCC threapies. Evidence IV.
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Objective: We documented the immunological profile of neonates and mothers, and lymphocyte subsets at birth. Methods: Consecutively born preterm neonates (26 to 31 weeks gestation) at our level III neonatal unit, fulfilling the inclusion criteria were enrolled. Immunoglobulin levels were assessed in maternal blood and in cord blood along with T cell subsets. Results: A total of 115 neonates were enrolled. The mean cord levels for IgG, IgM and IgA, respectively were 5.34, 0.10 and 0.04 g/L and of B, T, NK and NK-T cells were 14%, 71%, 10% and 1%, respectively of total lymphocyte population. Cord IgG and IgA levels showed a significantly rising trend with increasing gestation (P=0.005 and 0.02, respectively) but not IgM and T cell subsets. Maternal immunoglobulins were similar in all gestations. Conclusion: The cord IgG and IgA increased with increasing gestation but not IgM in neonates.
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Extranodal nature killer/T cell lymphoma (ENKTCL) is a highly aggressive non-Hodgkin lymphoma (NHL) with a high mortality. ENKTCL is epidemic in Asia and Latin America, especially in China, Japan and South Korea. EBV has been proved to be associated closely with ENKTCL; however, specific molecular mechanism which can explain how the neoplasm develops is still unclear. Chinese population seems to be more susceptible to this lymphoma and Chinese scientists have made great contributions to the pathogenesis and the treatments of ENKTCL. This review mainly introduces the recent progress of molecular pathogenesis of ENKTCL.
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El objetivo de esta revisión es analizar el estado actual de nuestro conocimiento sobre las moléculas de superficie celular involucradas en la presentación de antígenos glicolipídicos, denominadas familia CD1. Estas proteínas constituyen la tercera clase de moléculas presentadoras de antígeno. Las proteínas CD 1 controlan diversas funciones inmunes importantes en la defensa del hospedero contra las infecciones microbianas. En años recientes estas proteínas han sido involucradas en la generación de una respuesta inmune celular contra Mycobacterium tuberculosis. Aquí, nosotros analizaremos aspectos relevantes acerca de las proteínas CD 1 y las células T específicas para antígenos glicolipídicos.
The aim of this review is to analyze the current state of our knowledge about cell surface molecules involved in glycolipid antigen presentation, named CD1 family. These proteins constitute a third class of antigen-presenting molecules. CD 1 molecules develop diverse important immune functions in host defenses against microbial infections. In recent years these proteins have been involved in the generation of cell-mediated immune response against Mycobacterium tuberculosis. Here, we analyze relevant roles of CD1 proteins and glycolipid antigen-specific T cells.