ABSTRACT
Objective; To screen the genes may be regulated by NOB1 by using gene microarray technique, and to clarify the regulatory effect of NOB1 on the expression of osteosarcoma cell-related genes. Methods: The U20S cells were treated with lentivirus-mediated RNA interference and to establish the osteosarcoma cells Lv-shN0Bl-U20S. Lv-shCon-U20S group and Lv-shN0Bl-U20S group were set up. The mRNA expressions of those cells were detected using expression pattern analysis. Gene Oncology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to predict the global functions of NOB1, including biological processes, cellular components, molecular functions, and signaling pathways. Results; After NOB1 interference in the U20S cells, there were 792 genes with up-regulated mRNA expression level and 1 059 genes with down-regulated mRNA expression level, with a total variation of 1 851 genes. The GO analysis results showed that from the enrichment degree of cell location entries, the differentially encoded product proteins were mainly distributed on the cell membrane as 56. 9% of the total difference genes, 39. 4% of the totally differential genes distributed in the extracellular region, and 20% of the totally differential genes in the extracellular space; from the enrichment degrees of the molecular function items, the main function of the differentially encoded product proteins was calciu mion binding-related function (22% of the totally differential genes), the second function was transporter activity (9. 2% of the totally differenital genes), and the third function was actin binding activity (8. 7% of the totally differential genes). In terms of the enrichment of biochemical process entries, the main participation process of differentially encoded product proteins was 18. 7% of the totally differential genes of signal transduction, the second involved process was 15. 6% of the totally differential genes produced by multiple organelles, and the third process was the cell adhesion process accounted for 10. 2% of the totally differential genes. The KEGG analysis results showed that their encoding proteins were involved in plasma membrane, calcium ion binding activity and signal transduction. Conclusion; Knockout of NOB1 can affect the expressions of osteosarcoma cell-related genes in an all-round way.
ABSTRACT
NOB1 is a newly found gene regulating the function of the 26S proteasome, cell cycle and gene transcription. NOB1 contains a zinc ribbon domain and a PIN domain. Recent studies have indicated that the PIN domain is related to gene transcription and the zinc ribbon domain plays an important role in regulating cell cycle. It has also been found that NOB1 is closely associated with development and progression of gastric, esophageal, and ovarian cancer. The paper reviews the role of NOB1 in the development and progression of tumors.