ABSTRACT
Objective: To optimize the formula of artificial diets for Poecilobdella manillensis. Methods: Survival rate and individual body weight growth rate of P. manillensis were chosen as indexes. The three factors [NP-90 spray-dried hemoglobin powder, NP-2002 spray-dried plasma powder, and vitamin C (VC)] of the formula of artificial diets were investigated by L9(34) orthogonal test. AHP was applied to statistical analysis on non-interacting orthogonal test, the effect weight of each factor for test result was obtained, and principal-secondary sequence of factors and optimal project was given. Results: The effects of the three components in artificial diets on the survival rate were in the following decreasing order: VC>NP-90>NP-2002. And the optimization of components of artificial diets was 0 NP-90 spray-dried hemoglobin powder, 1% NP-2002 spray-dried plasma powder, and 0.05% VC. When the individual body weight growth rate was chosen as the index, the effects of the three components in artificial diets on the individual body weight growth rate were in the following decreasing order: NP-2002>NP-90>VC. The optimization of components of artificial diets was 0.5% NP-90 spray-dried hemoglobin powder, 3% NP-2002 spray-dried plasma powder, and 0.05% VC. The weight of principal-secondary sequence of factors and levels influences on the tests' results was NP-90>NP-2002>VC. The weight of 0.5% NP-90 spray-dried hemoglobin powder, 3% NP-2002 spray-dried plasma powder and 0.05% VC was maximum. And the optimization of components in artificial diets was 0.5% NP-90 spray-dried hemoglobin powder, 3% NP-2002 spray-dried plasma powder, and 0.05% VC. According to the results, P. manillensis bred with the artificial diets (0.5% NP-90 spray-dried hemoglobin powder, 3% NP-2002 spray-dried plasma powder, 3% NP-2002 spray-dried plasma powder, 0.05% VC, and other components) had the survival rate of 100% and the individual body weight growth rate was 19.91%. Conclusion: The optimal artificial diets could be used to feed P. manillensis.