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Objective To determine the aberrant methylation status of TFPI-2,FOXE1 and NPTX2 gene promoter region in bile from cholangiocarcinoma patients and the diagnostic value for cholangiocarcinoma. Methods Methylation—specific PCR(MSP)was used to detect the promoter methylation status of TFPI-2, FOXE1 and NPTX2 genes in bile from 29 patients with cholangiocarcinoma and 27 patients with choledocholi-thiasis.The sensitivity and specificity of combined methylation of 3 promotors and brush cytology were analyzed. Results There was significant difference between the methylation rate of NPTX2,FOXE1 in choledocholithiasis group and cholangiocarcinoma group(75. 86% VS 25. 93%,χ2 = 13. 964,P0. 05).Positive rate was 34. 48% with endoscopic retrograde brush cytolog,and 86. 20% with promoter methylation of FOXE1 combined with NPTX2 genes,and there was significance difference in the positive rates between the two methods(χ2 = 14. 122,P< 0. 05). Conclusion The methylation rates of NPTX2,FOXE1 were significantly higher in choledocholithiasis group than those in the cholangiocarcinoma group. Detection of comethylation rate of the FOXE1 and NPTX2 genes could improve diagnostic sensitivity of cholangiocarcinoma.
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Objective: To investigate the methylation level of NPTX2 gene in pancreatic cancer and assess its clinical value in diagnosis of pancreatic cancer. Methods: We detected the mRNA expression and methylation levels of NPTX2 gene by qRT-PCR and quantitative methylation with SYBR Green in 10 primary pancreatic carcinoma tissues and the matched adjacent normal tissues. The methylation levels of NPTX2 gene were then detected and compared using the above-mentioned method in the whole blood of patients with pancreatic ductal adenocarcinoma and healthy volunteers. Results: The relative quantification (RQ) of NPTX2 expression in pancreatic carcinoma tissues was significantly lower than that in the adjacent tissues (0.276±0.263 vs 3.526±3.037, P = 0.001). The result of quantitive methylation indicated that the methylation index (MI) of the carcinoma tissues was significantly higher than that of the adjacent tissues ([9.02±7.52]% vs [1.28±0.98]%, P = 0.003). MI of NPTX2 gene was negatively correlated with RQ value (R= -0.552, P = 0.012). We also found that the MI of the whole blood DNA methylation of patients with pancreatic cancer was significantly higher than that of healthy volunteers ([1.80±1.76]% vs [0.84±0.45]%, P < 0.05). Conclusion: Our findings strongly suggest that NPTX2 gene is aberrantly hypermethyated in pancreatic ductal adenocarcinoma. Detection of MI of NPTX2 gene might be of great value for diagnosis of pancreatic cancer.
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Neuronal pentraxin II (NPTX2) is a member of neuronal pentraxin family, but its distribution is not limited to the nervous system. Recent studies have shown that NPTX2 is associated with a number of diseases, including malignant gliomas, Parkinson's disease, narcolepsy, drug addiction, lung cancer, and pancreatic cancer. This article reviews the roles of NPTX2 in diseases of the nervous system and other systems.
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Objective To construct eukaryotie vector of human neronal pentraxin 2 (NPTX2),and obtain the sstable transfected PANC1 cell lines.Metods The full-length cDNA of NPTX2 was diget EcoRl and Notl,and was cloned into plasmid pcDNA3,1,which were confirmed by sequencing,then the PcDNA3,1(+)and pcDNA3,1(+)-NPTX2 vectors were transfected into PANC1 cells by LipofectamineTM 2000,The stable transfected PANC1 cell lines were selected by the abiliy of resistanc to G418.The NPTX2 mRNA expression of PANC1 in the selected clones was detected by real-time quantitative PCR.Results The eukaryotic vector pcDAN3,1(+)-NPTX2 was constructed successfully,stable transfected PANC1 cell line was established and confirmed by real-time quantitative PCR.Conclusions The construction of eukaryotic vector targeting NPTX2 and the established sstable transfected PANC1 cell line provided a solid experimental foundation for further studies on the function of NPTX2 in pancreatic cancer cells.