ABSTRACT
Objective To determine the purity of sodium nitrite(NaNO2) using in stray light standard substance which was applied to check semiautomatic clinical chemistry analyzer.Methods Titration was used to determine the purity of NaNO2,the uncertainty of the purity was assessed,and a parallel test was used to validate the uncertainty.Results The purity of NaNO2 was 99.81%,and its uncertainty was 0.86%.Conclusion The uncertainty of the purity passes the validation,it shows that the purity test is credible,and its uncertainty assessment is logical.
ABSTRACT
Objective This study evaluates the Anti- hypoxic effect of Fo li um Bambusae extract (FBE). Methods The survival time of mice under the conditi on of closed normobaric hypoxia, and those subjected to KCN, NaNO2 and lidoc aine poisoning, and the time of electrocardiograph disappearance after occlusio n of trachea and the duration of continuous gasping in mice after decapitation were used to evaluate the Anti- hypoxic effect of FBE. Results FBE ( 22.5 m g/kg, 45.0 mg/kg, 90.0 mg/kg) prolonged the survival time of mice under the c ondition of closed normobaric hypoxia, prolonged the survival time in mice subj ected to KCN, NaNO2 and lidocaine poisoning and prolonged the time of electro cardiograph disappearance and the duration of gasping in decapitated mice. Conc lusion FBE can increase the anti- hypoxic effect of animals.
ABSTRACT
The effects of 4 efficacious cyanide antidotes, 4-dimethylaminophenol (4-DMAP) , P-aminopropiophenone hydrochloride (PAPP. HC1), NaNO2, and di-cobalt ethyleneamino-tetraacetate (Co2-EDTA), on the liver and kidney functions and on the blood gases and serum levels of lactate and potassium were investigated in rabbits. The former 3 agents can convert hemoglobin into me-themoglobin, and the dosages that are required to convert 30~35% of total hemoglobin into methemoglobjn are 15mg/kg, 35mg/kg, and 55 mg/kg for 4-DMAP, PAPP . HC1 and NaNO2 respectively. Obviously, 4-DMAP is the most potent one. Co2-EDTA can directly combine with cyanides to form nontoxic complex without affecting hemoglobin.According to the results of the 2-hour observation on the acute experiments in rabbits, all the 4 agents exert no harmful effects on the liver functions. 4-DMAP and PAPP. HC1 are also not harmful to the kidneys. But serum urea nitrogen was increased to 204% above the normal 30 minutes after intravenous NaNO2 (110 mg/kg) , and to 171% 90 minutes after intravenous Co2-EDTA(40 mg/kg) . Apparently, both the agents are injurious to the renal functions.4-DMAP can decrease the blood level of lactate. NaNO2 exerts most marked effect on the serum level of potassium. After the administration of 55 mg/kg and 110 mg/kg of NaNO2/the serum potassium level was increased 2.5 and 4.5 fold respectively. The larger the dose of NaNO2, the higher the serum potassium level, and more quickly the elevation of potassium level occurs.4-DMAP has a transient action on the arterial Po2 which usually returns to normal in 30 minutes. The action of NaNO2 and PAPP. HC1 on the blood gases are essentially similar, they can quickly lower the arterial Po2 but its recovery to normal is sluggish.
ABSTRACT
The effects of 4-DMAP and NaNO2 on the hemodynamics of dogs suffering from acute hemorrhage complicated with cyanide poisoning were studied. It was found that the administration of 4-DMAP could brought about an increase of the cardiovascular functions in the experimental animals. Although the increase was temporal and not very impressive, it played an important role to prevent the dogs from developing cardiovascular collapse during the period of observation. On the other hand, the administration of NaNO2 resulted in a transient excitation for 1-2 minutes and then a prolonged and progressive depression of the cardiovascular functions, and all the experimental animals died from cardiovascular failure within 13-19 minutes after NaNO2 injection.The results of this study indicate that 4-DMAP as a therapeutic agent for acute hemorrhage complicated with cyanide poisoning is superior to NaNO2 since the former can produce an excitation of the cardiovascular functions while the latter a progressive depression and eventually a failure of the cardiovascular functions.