Enhanced antitumor effect of curcumin loaded solid lipid nanoparticles in Dalton’s ascites lymphoma mice

Curcumin is widely known for its antibacterial, antioxidant and anti inflammatory effects and has been reported to possess anticancerous activity as well. However, its medical application is limited because of poor bioavailability and rapid metabolism. In this study, we encapsulated curcumin in solid lipid nanoparticles and studied its anticancerous effect in Dalton’s Ascites Lymphoma (DAL) mice model. The physicochemical characteristics of curcumin solid lipid nanoparticles (CUR-SLN) were assessed and the anticancer efficacy was determined by in vivo studies. The curcumin solid lipid nanoparticles were synthesized by solvent emulsification evaporation method with particle size less than 100 nm. Antitumor effect of nanocurcumin (50 mg/kg) and curcumin (100 mg/kg) was evaluated in Dalton’s Ascites Lymphoma bearing mice. Pathological and immunohistochemical parameters were studied. Mean survival time and percentage increase in lifespan were assessed. Nanocurcumin group showed more significant influence in reducing tumor volume and weight, inducing apoptosis, reducing angiogenesis and invasion restoring antioxidant parameters and increased mean survival time. Curcumin and nanocurcumin inhibited the activation of nuclear factor-kappa B (Nf-kB), and thereby proved the pathway by which it induced anti-angiogenic and anti-invasive property.

Synergistic effects of dendrosomal nanocurcumin and oxaliplatin on oncogenic properties of ovarian cancer cell lines by down-expression of MMPs

BACKGROUND: Contrary to the advantageous anticancer activities of curcumin (Cur), limited bioavailability and solubility hindered its efficacy. Here, nontoxic dendrosomal nano carrier with Cur was used to overcome these problems. Despite considerable antitumor properties of Oxaliplatin (Oxa), the limiting factors are drug resistance and adverse side-effects. The hypothesis of this study was to evaluate the possible synergism between dendrosomal nanocurcumin (DNC) and Oxa and these agents showed growth regulatory effects on SKOV3 and OVCAR3 cells. METHODS: and materials In the present study, colony formation, wound healing motility, cell adhesion, transwell invasion and migration assay and cell cycle arrest with or without DNC, Oxa and Combination were defined. In addition to, real time PCR and Western blot were used to analyze AKT, PI3K, PKC, JNK, P38 and MMPs mRNAs and proteins expressions. Docking of MMP-2-Cur, MMP-2-DNC and MMP-2-Oxa was performed and the results of all three complexes were simulated by molecular dynamics. RESULTS: Our findings illustrated that DNC had the greatest effect on cell death as compared to the Cur alone. Moreover, the growth inhibitory effects (such as cell death correlated to apoptosis) were more intense if Oxa was added followed by DNC at 4 h interval. However, insignificant effects were observed upon simultaneous addition of these two agents in both cell lines. Besides, a combination of agents synergistically alters the relative expression of MMP-9. CONCLUSIONS: The docking results showed that His70 and Asp100 may play a key role at the MMP-2 binding site. The matrigel invasion as well as cell viability of ovarian cancer cell lines SKOV3 and OVCAR3 by DNC alone or in combination with Oxa was inhibited significantly. The inhibitory effects of these agents were due to the differential expression levels of MMP 2 and MMP 9 regulated by multiple downstream signaling cascades. From the molecular dynamic simulation studies, it was confirmed that DNC established a strong interaction with MMP-2.

Evaluation of Nanocurcumin Mouthwash as an Adjunctive Plaque Control Agent: A Clinical Pilot Study

The use of adjunctive plaque control methods such as mouthwashes has shown to be effective in prevention of plaque accumulation. The gold standard for mouthwashes is 0.2% chlorhexidine, however, various side effects compels researchers to divert towards herbal alternatives. Curcumin has been enhanced with nanotechnology to enhance its efficacy and water solubility. The aim of study was to compare the efficacy of 0.1% nanocurcumin mouthwash to 0.2% chlorhexidine gluconate as an adjunct to scaling and root planning in localized chronic periodontitis patient. In this randomized controlled clinical study, a total of 30 patients having localized mild to moderate periodontitis were included. The study population was divided into 2 groups by randomization protocol; Group A (n=15) was given 0.1% nanocurcumin mouthwash whereas Group B (n=15) was given 0.2% CHX mouthwash. Clinical parameters including Plaque index (PI), Modified Gingival Index (mGI) being recorded at baseline, 30th day and 45th day and Sulcular Bleeding Index (SBI), Periodontal Probing Depths (PPD), Clinical attachment levels (CAL) were recorded at baseline and 45th day. Subjective criteria included taste acceptability, burning sensation and dryness whereas objective criteria including ulcer formation, tongue and teeth staining were analyzed. Statistically significant improvement was observed in all clinical parameters when compared to baseline in both groups and difference was statistically non-significant on intergroup comparison. No adverse reaction was observed in both groups in terms of subjective and objective criteria. Within the limitations, it can be concluded that nanocurcumin can be a viable alternative to chlorhexidine to formulate a mouthwash