ABSTRACT
Objective To research the chemical constituents distribution from the exocarps of Jugland mandshurica in rats' stomach based on UPLC-Q-TOF/MS technology. Methods After ig administration with the exocarps of J. mandshurica, the tissue samples of stomach collected from each rat. Tissue samples were treated with organic solvent for protein precipitation. Peakview 2.0/masterview 1.0 and Metabolitepilot1.5 software were used to determine the constituents from the exocarps of J. mandshurica based on the acquired accurate mass, isotope abundance, and MS/MS fragment mass spectra. Results In total, 54 constituents were identified, including 41 prototypes and 13 metabolites. The prototypes consists of 17 naphthoquinone, 9 diarylheptanoids, 7 flavonoids, 5 triterpenes, and 3 phenolicacids. And metabolites consists of 4 naphthoquinones, 3 diarylheptanoids, 1 flavonoids, and 5 other main components. Conclusion The work was deeply researched the constituents from the exocarps of J. mandshurica in rats' stomach for the first time based on UPLC-Q-TOF/MS technique. It was demonstrated that this method could be successfully applied for rapid screening and deeply analysis of chemical constituents in vitro. This research provides scientific research data for exocarps of J. mandshurica against gastric cancer.