ABSTRACT
Objective • To explore the effect of intrauterine high-nutrition on cytokine expression in umbilical cord blood-derived monocytes and nature killer (NK) cells. Methods • Samples of umbilical cord blood were collected from macrosomia (n=10) and infants with normal birthweight (n=18). Monocytes and NK cells were obtained by magnetic sorting. Purified monocytes were stimulated by lipopolysaccharide (LPS), while purified NK cells were stimulated by phorbol myristate acetate (PMA) and ionomycin (ION). The expression of cytokines was detected by real-time PCR and ELISA. Results • There was no significant difference in cytokine expression in the monocytes treated with PBS between macrosomia and infants with normal birthweight. However, dampened responses following LPS stimulation were observed in macrosomia group, including the mRNA and protein expression of IL-1α, IL-1β, TNF-α, IL-10 and CCL5. Moreover, mRNA and protein expression of TNF-α were significantly higher in NK cells from macrosomia group, while no significant difference in IFN-γ was found. Conclusion • Intrauterine high-nutrition might result in dampened response in monocytes following LPS stimulation and exaggerated expression of TNF-α in NK cells.
ABSTRACT
Objective@#To study the functional effects of killer cell lectin-like receptor subfamily G member 1 (KLRG1) expression on natural killer cells (NK cell) in chronic hepatitis B virus infection (HBV).@*Methods@#Peripheral blood mononuclear cells (PBMC) were extracted from 120 patients with chronic hepatitis B virus infection and 19 healthy persons. The frequency of NK cells and KLRG1+ NK cells in peripheral blood was detected by flow cytometry. Interferon-γ levels secreted by NK cells were detected in peripheral blood. Statistical analysis of experimental data was performed using GraphPad Prism 6.03 software.@*Results@#The frequency of NK cells in HBV-infected group (16.92% ± 7.9%) was not significantly different from that in healthy controls (10.57% ± 6.5%). The frequency of KLRG1+NK cells in HBV-infected group was significantly higher (49.43% ± 21.2%) than that to healthy control group (31.60% ± 17.9%), (t = 7.347 6, P < 0.001). IFN-γ secretion of KLRG1 + NK cells in HBV-infected patients (2.59% ± 1.0%) were significantly lower than healthy controls (5.96% ± 2.4%), (P = 0.009).@*Conclusion@#HBV infection can increase the expression of KLRG1 in NK cells and further reduce the secretion of IFN-γ in NK cells, which may be an important cause for chronic HBV infection.
ABSTRACT
Objective:To investigate the effect of HLA genetic susceptibility and NK cell receptors and immune response on the occurrence and development of the Cervical cancer.Methods: Select the 200 patients confirmed by the pathological biopsy in our hospital from January 2013 to January 2014 as the observation group.At the same time,randomly select the 200 healthy women as the control group.Both of them blood 2 ml peripheral blood,sample the cervical cell from the observation group.Having the cytological ob-servation and the DNA′s probe of the HPV,observe two group′s HPV infection rates and HLA′s parting.Results: The HPV infection rates of the observation group is 91%,and the rates of the control group is 16%.The differences between them were all significant(P<0.05).The HLA-KIR*1003,HLA-KIR*14,HLA-KIR*17,HLA-KIR*02,HLA-KIR*12 distribution frequency of the observation group are 41%,39%,35%,15%,53%.The HLA-KIR*1003,HLA-KIR*14,HLA-KIR*17,HLA-KIR*02,HLA-KIR*12 distribution frequency of the control group are 18%,15%,14%,52%,89%.The differences between them were all significant ( P<0.05).Among them The HLA-KIR*1003, HLA-KIR*14, HLA-KIR*17 distribution frequency of the observation group are significantly higher than the control group, HLA-KIR*02 , HLA-KIR*12 distribution frequency of the observation group are significantly lower than the control group.Conclusion:During the occurrence and development of the Cervical cancer,the HLA-KIR*1003,HLA-KIR*14,HLA-KIR*17 may be the risk factors for the Cervical cancer;the HLA-KIR*02,HLA-KIR*12 may be the protective factors for the Cervical cancer.
ABSTRACT
Myeloid-derived suppressor cells are a heterogeneous population of early myeloid progenitors,immature granulocytes,macrophages,and dendritic cells at different stages of differentiation.These cells have the capacity to suppress both the innate immunity response mediated by the cytotoxic natural killer cells and natural killer T cells,and the adaptive immune response mediated by CD4+ and CD8+ T cells.In addition,myeloid-derived suppressor cells have close links with macrophages,dendritic cells,regulate T cells and so on,and also play an important role in the process of tumor progression.