ABSTRACT
Objective: To establish a method for the analysis of constituents of Pheretima aspergillum by UPLC-Q-TOF-MS. Methods: The analysis was performed on an Acquity UPLC BEH C18 column (100 mm×2.1 mm, 1.7 μm) by gradient elution. The mobile phase consist of 0.1% formic acid-acetonitrile and 0.1% formic acid-water at a flow rate of 0.3 mL/min. The column temperature was at 35 ℃. The information of the compounds was acquired in positive and negative mode. Results: Total 83 compounds were inferred, contains 11 free amino acids, 26 organic acids, 11 necleosides, 5 dipeptides and cyclic dipeptideds and other 21 nitrogen-containing substances, the rest on a total of 10. Conclusion: The method is accurate, rapid, and sensitive, and provide a basis for further clarifying the material basis of its efficacy and the selection of quality control indicators.