ABSTRACT
Objective: To investigate the chemical constituents from the whole plants of Gynura procumbens. Methods: The chemical constituents were isolated and purified by repeated silica gel column chromatography, Sephadex LH-20 gel column chromatography, medium pressure column chromatography, and semi-preparative HPLC, and their structures were elucidated by chemical properties and spectroscopic analyses. Results: Sixteen compounds were identified to be quercetin (1), apigenin (2), luteolin (3), kaempferol (4), astragaline (5), kaempferol-5-O-(6″-O-acetyl)-β-D-glucopyranoside (6), negletein (7), 4-methoxycinnamic acid (8), benzyl-O-β-D-glucopyranoside (9), 2-phenylethyl-O-β-D-glucopyranoside (10), 3,5-dicaffeoylquinic acid methyl ester (11), 3,5-dicaffeoylquinic acid ethyl ester (12), 3,4-dicaffeoylquinic acid methyl ester (13), 4,5-dicaffeoylquinic acid methyl ester (14), protocatechuic acid (15), and eugenol glucoside (16). Conclusion: Compounds 7, 8, 12, 14, and 16 are obtained from the plants in Gynura Cass. for the first time, and compounds 3, 6, 9-11 and 13 are obtained from this plant for the first time.
ABSTRACT
AIM:To establish a method for simultaneously determining luteolin and negletein in Mosla chinensis Maxium collected in picking times.METHODS: The Angilent Zorbax,Extend C-18 column(4.6 mm×250 mm,5μm) was used with methanol-water (containing 0.4% phosphonic acid)(50: 50) as mobile phase.The flow rate was 1.0 mL/min.The detection wavelength was set at 350 nm.RESULTS : The method had the good linear relationship within the range of 0.04-0.61 μg for luteolin and within the range of 0.20-3.01 μg for negletein,respectively,with the correlation coefficient of 0.999 9.The average recoveries of the two flavones were 97.32%,98.60%,respectively.The contents of luteolin and negletein in Mosla chinensis Maxium.varied in different picking times,which reached the its maximum at florescence (264.8 μg/g,2 746.3 μg/g) in August,respectively.CONCLUSION: The method is fast,rapid,convenient,accurate and can be used for quality control of Mosla chinensis Maxium.
ABSTRACT
AIM:To establish a method for simultaneously determining luteolin and negletein in Mosla chinensis Maxium collected in picking times.METHODS:The Angilent Zorbax,Extend C-18 column(4.6 mm?250 mm,5 ?m)was used with methanol-water(containing 0.4% phosphonic acid)(50 ∶50)as mobile phase.The flow rate was 1.0 mL/min.The detection wavelength was set at 350 nm.RESULTS:The method had the good linear relationship within the range of 0.04-0.61 ?g for luteolin and within the range of 0.20-3.01 ?g for negletein,respectively,with the correlation coefficient of 0.999 9.The average recoveries of the two flavones were 97.32%,98.60%,respectively.The contents of luteolin and negletein in Mosla chinensis Maxium.varied in different picking times,which reached the its maximum at florescence(264.8 ?g/g,2 746.3 ?g/g)in August,respectively.CONCLUSION:The method is fast,rapid,convenient,accurate and can be used for quality control of Mosla chinensis Maxium.