ABSTRACT
OBJECTIVE: To develop an UPLC-MS/MS method for the determination of neopanaxadiol(NPD) in rat plasma sample, and to investigate plasma protein binding rate of NPD in rats. METHODS: Under three different concentrations, equilibrium dialysis method was utilized to imitate the binding process between NPD and plasma protein. The concentration of NPD in and out of the dialysis membrane was determined by UPLC-MS/MS and the plasma protein binding rate of NPD were calculated. RESULTS: Excellent linearity was found between 0.05-8 μg·mL -1. Intra- and inter-day precision values (RSD) of QC samples were both below 15% and the extraction recoveries of NPD from biological matrices were better than 79.37%. The plasma protein binding rates of NPD were (86.55±4.50)%, (76.50±2.61)% and (78.25±1.32)% at low, middle and high concentrations, respectively. There was no significant difference among three concentrations. CONCLUSION: These results indicate the high plasma protein binding rates of NPD in plasma in combination mode.
ABSTRACT
OBJECTIVE: To develop a UPLC-MS/MS method for the determination of neopanaxadiol (NPD) in rat urine samples, and to explore the excretion patterns of NPD in rats after oral administration. METHODS: NPD was extracted from u-rine samples by liquid-liquid extraction. The concentration of NPD in urine was determined by UPLC-MS/MS and the cumulative excretion amount and excretion rate of NPD were calculated after a single oral administration of NPD at 100 mg · kg-1 to SD rats. RESULTS: Excellent linearity was found between 80-1280 ng · mL-1. The intra-and inter-day RSDs of the QC samples were both below 15% and the extraction recoveries of NPD were higher than 80%. The cumulative excretion of unchanged NPD in urine within 96 h amounted to (0.0233±0.0356)% of the dose. CONCLUSION: NPD is hardly eliminated through urine within 96 h in rats.