ABSTRACT
Objective To investigate the expression and clinical significance of ATAD2 in patients with hilar cholangiocarcinoma (HC) Methods The qRT-PCR,Western blottig and immunohistochemistry were used to detect the expression of ATAD2 in HC and normal bile duct tissues,and the relationship between ATAD2 and the clinicopathological factors,prognosis was analyzed.Results The positive expression rate of ATAD2 in HC tissues was 70％ (57/81),significantly higher than that of normal bile duct tissues 7％ (2/30) (x2 =35.678,P ＜0.01),and ATAD2 expression was associated with lymph node metastasis and TNM staging (respectively x2 =28.619,31.612,all P ＜ 0.01).The 1'-,3'-,and 5-year survival rates of ATAD2 negative HC patients were 87.5％,54.1％,and 33.3％,respectively.The 1'-,3'-,and 5-year survival rates of ATAD2 positive HC patients were 40.3％,14.0％ and 1.7％,respectively (x2 =14.162,P ＜ 0.01).Univariate analysis and multivariate COX regression model analysis showed that microvascular invasion,lymph node metastasis and ATAD2 expression were independent factors influencing the prognosis of patients with HC (respectively F =4.703,4.961,5.013,all P ＜ 0.05).Conclusion The expression of ATAD2 in HC increased,and the overexpression of ATAD2 was closely related to the occurrence,development,invasion,metastasis and poor prognosis of HC.
ABSTRACT
Objective To study the clinicopathological significance and biological behavior of human colorectal cancer (CRC) with the expression of angiopoietin-like protein 1 (ANGPTL1).Methods The expression of ANGPTL1 protein in 98 paraffin embedded CRC specimens and paired adjacent non cancerous tissues were detected by immunohistochemistry.The relationship between the protein expression and clinicopathological features was analyzed.Western blot and qRT-PCR were used to examine the expression of ANGPTL1 protein and mRNA levels in 24 paired fresh CRC and adjuvant non-cancerous tissues.Finally,ANGPTL1 expression in CRC cell lines with different metastatic capabilities was detected by WB.Cell invasion and migration were detected using ANGPTL1 siRNA.Results ANGPTL1 expression in CRC tissues was much lower than that in non-cancerous tissues (44％ vs.76％,P ＜ 0.01).ANGPTL1 expression was negatively associated with T stage (x2 =5.766,P =0.016),lymph node metastasis (x2 =5.571,P =0.018) and TNM stage (x2 =7.773,P =0.005).Moreover,CRC patients with ANGPTL1 positive expression had a better prognosis.ANGPTL1 protein and mRNA levels in CRC tissues were higher than in non-cancerous tissues (t =3.126,P =0.005;t =2.523,P =0.019).ANGPTL1 interference promoted cell invasion and migration of HCT116 cells.Conclusion ANGPTL1 expression is closely associated with tumor T stage,lymph node metastasis,TNM stage and worse prognosis of CRC patients.ANGPTL1 inhibits cell invasion and migration of CRC cells.