The protective effect of curcumin on the lipid peroxidation caused by lead poisoning in rat / 中华实用儿科临床杂志

Objective To evaluate the effect of curcumin on the lead burden in lead－exposed rats,and to study whether curcumin can influence the lipid peroxidation caused by lead exposure. Methods A total of 70 rats of 21－day－old rats were divided randomly to 7 groups,the control group was given normal diet and drinking water,the curcumin group was given curcumin only 1 month after normal drinking water being given;the other 5 lead－exposed groups were given 2 g/L of acetate lead in free drinking water for 1 month and then randomly divided into lead－ex﹣posed group,low,medium and high concentration curcumin groups and Dimercaptosuccinic acid( DMSA)group,rela﹣tively. Lead levels of lead－exposed rats were determined by using inductively coupled plasma mass spectrometry,and commercial kit was used to detect antioxidant enzymes,and glutathione related enzymes and lipid peroxides. Results The lead concentrations in the blood,hippocampus,liver and kidney of lead－exposed group increased,the levels of each group were(221. 76 ± 12. 59)μg/L,(1. 10 ± 0. 11)μg/g,(1. 40 ± 0. 12)μg/g,(8. 26 ± 0. 47)μg/g, and(57. 58 ± 6. 09)μg/g,respectively;compared with the lead－exposed group,the lead concentrations reduced in the blood,hippocampus,liver and kidney significantly(F﹦90. 67,39. 07,27. 34,86. 04,all P﹦0. 000)in the curcu﹣min－treated group,while the effect of curcumin in the bones showed no significant difference between groups( F﹦5. 65,P﹦0. 230). Lead could significantly improve the level of lipid peroxides in the serum and hippocampus,and cur﹣cumin－treated groups could significantly reduce the level of lipid peroxidation( F﹦58. 03,19. 25,32. 27,24. 83,all P﹦0. 000)and(F﹦28. 18,33. 71,38. 95,32. 11,all P﹦0. 000);lead could also reduce the antioxidant enzyme activity in the serum and hippocampus;curcumin －treated groups could significantly increase the antioxidant enzyme activity (F﹦18. 24,78. 65,all P﹦0. 000)and(F﹦13. 68,17. 04,all P﹦0. 000),respectively. The concentration in the curcu﹣min group was better than that of DMSA group(P＜0. 05). Conclusions Curcumin can downregulate the lead concen﹣trations in the blood,hippocampus,liver and kidney and improve the activity of antioxidant enzymes,inhibit the oxida﹣tive stress induced by lead,and thus resist the lead－induced damage.

The protective effect of curcumin on the lipid peroxidation caused by lead poisoning in rat / 中华实用儿科临床杂志

Objective@#To evaluate the effect of curcumin on the lead burden in lead-exposed rats, and to study whether curcumin can influence the lipid peroxidation caused by lead exposure.@*Methods@#A total of 70 rats of 21-day-old rats were divided randomly to 7 groups, the control group was given normal diet and drinking water, the curcumin group was given curcumin only 1 month after normal drinking water being given; the other 5 lead-exposed groups were given 2 g/L of acetate lead in free drinking water for 1 month and then randomly divided into lead-exposed group, low, medium and high concentration curcumin groups and Dimercaptosuccinic acid(DMSA) group, relatively.Lead levels of lead-exposed rats were determined by using inductively coupled plasma mass spectrometry, and commercial kit was used to detect antioxidant enzymes, and glutathione related enzymes and lipid peroxides.@*Results@#The lead concentrations in the blood, hippocampus, liver and kidney of lead-exposed group increased, the levels of each group were (221.76±12.59) μg/L, (1.10±0.11) μg/g, (1.40±0.12) μg/g, (8.26±0.47) μg/g, and (57.58±6.09) μg/g, respectively; compared with the lead-exposed group, the lead concentrations reduced in the blood, hippocampus, liver and kidney significantly (F=90.67, 39.07, 27.34, 86.04, all P=0.000) in the curcumin-treated group, while the effect of curcumin in the bones showed no significant difference between groups(F=5.65, P=0.230). Lead could significantly improve the level of lipid peroxides in the serum and hippocampus, and curcumin-treated groups could significantly reduce the level of lipid peroxidation(F=58.03, 19.25, 32.27, 24.83, all P=0.000) and (F=28.18, 33.71, 38.95, 32.11, all P=0.000); lead could also reduce the antioxidant enzyme activity in the serum and hippocampus; curcumin-treated groups could significantly increase the antioxidant enzyme activity (F=18.24, 78.65, all P=0.000) and (F=13.68, 17.04, all P=0.000), respectively.The concentration in the curcumin group was better than that of DMSA group (P<0.05).@*Conclusions@#Curcumin can downregulate the lead concentrations in the blood, hippocampus, liver and kidney and improve the activity of antioxidant enzymes, inhibit the oxidative stress induced by lead, and thus resist the lead-induced damage.

The Changes of Regional Cerebral Blood Flow according to Inhalational Anesthetic Agents after Transient Bilateral Carotid Artery Occlusion in the Rabbit / 대한구급학회지

BACKGROUND: Cerebral hyperperfusion syndrome is a recognized complication of carotid endarterectomy, with a reported incidence of 0.3 to 1.2%. Monitoring of regional cerebral blood flow (rCBF) may limit neurological damage. This study was planned to investigate the changes of rCBF according to inhalational anesthetic agents. METHODS: 2.40+/-0.04 kg weighed New Zealand White Rabbits were undergone transient brain ischemia by bilateral carotid artery ligation for 20 minutes. The rCBF was measured by Bowman Perfusion Monitor. RESULTS: The value of rCBF in pre-ligation state was not significantly different among the three groups. rCBF in sevoflurane group was decreased to 46% of baseline value during ischemia and increased to 143% just after reperfusion. rCBFs in isoflurane and enflurane groups were abruptly increased instead of decrease like sevoflurane group. The values of rCBF was more increasing after reperfusion in isoflurane and enflurane groups. rCBFs in all groups were return to baseline value 10 minutes after reperfusion. CONCLUSIONS: This results was suggested that sevoflurane might be contributed to create a neurologic damage during ischemia and the hyperperfusion was seen in all three anesthetic agents. The clinical investigation may be needed to establish the value of this experiment.

A Case of Spinal Cord Injury without Radiographic Abnormality / 소아과

A spinal cord injury without radiographic abnormality(SCIWORA) was defined by Pang and Wilberger in 1982 as the "objective signs of myelopathy as a result of trauma" in which no evidence of fracture, subluxation, or instability on plain radiographs, or computed tomography is demonstrable. Inherent elasticity of the vertebral column in infants and young children, among other age-related anatomical peculiarities, render the pediatric bony cervical and thoracic spine exceedingly vulnerable to deforming forces. We report here on a case of SCIWORA in a 3-year-old girl who presented with lower extremity paralysis, paresthesia, and voiding difficulty. The mechanism of neural damage in this case was purely longitudinal distraction. Such stress on the spine resulted from severe pulling up the lower extremity by her father, which was not thought to be damaging at that time.