ABSTRACT
Objective: The study was to reveal the inhibition of neovascularization by Tongbiling. Methods: Rat model were established by injecting CFA at the root of the tail and divided into model control group, Indomethacin group and Tongbiling group at random. The serum and paws were collected after seven days treatment. 10ng/L VEGF solution was added I into CAM to stimulate the neovascularization, and to observe the influence of each group of serum on the first and second order new vessels. RT-PCR method was taken to determine the expression of mRNA of VEGF and VEGFR-1 of paws in different groups. Results: The number of vessels in the model control group was significantly more than that in the Indomethacin group and the Tongbiling group (P
ABSTRACT
Objective:To prepare a quantified animal model of retinopathy of prematurity by economic and easy method. Methods:A total of 44 postnatal day 7 wistar rats were randomly assigned into 2 groups according to different oxygen inhalation concentration(n=22).Experimental group was exposed to 75% oxygen for 5 days,and them deprived of oxygen supply suddenly and exposured in air for 5 days.The Control group was exposed to room air in same experiment condition.The retinal vascular development and proliferation were evaluated by stretched preparation (ADPase stained retina)and eyeball cross-section,The expression of vascular endothelial growth factor(VEGF) was detected by immunohisto-chemical stain.Results: The central vessels became tortuous and constricted and the central avascular area increased in high-concentration oxygen environ-ment.But hypoxia environment made great vessels of retina broaden and abnormal curvature become obvious,neova- scularization was seen in the Experimental group.The average numbers of neovascular nuclei which breakthrough limiting membrane of amphiblestrodes in the Experimental group and the Control group were 32.800 0?0.767 8 and 2.750 0?0.716 4 respectively.Stain of VEGF was weaker in the control group than in the treated group.Conclusion:The rat modle of retinopathy of prematurity has powerful repeatability,high constancy and can be used in quantitative study.It is a suitable mode to study the pathogenesis and therapy of retinopathy of prema-turity.