ABSTRACT
OBJECTIVE:To establish a method for simultaneous determination of ephedrine hydrochloride,nitrofural and di-phenhydramine hydrochloride in Compound diphenhydramine nasal drop. METHODS:RP-HPLC method was adopted. The determi-nation was performed on Inertsil ODS-3 C18 column with mobile phase consisted of 0.05 mol/L potassium dihydrogen phosphate buf-fer(pH 7.0)-acetonitrile(gradient elution)with flow rate of 1.0 mL/min. The detection wavelength was set at 256 nm,and the col-umn temperature was 35 ℃. The sample size was 20 μL. RESULTS:The concentrations of phedrine hydrochloride,nitrofural and diphenhydramine hydrochloride ranged 122.1-366.3 μg/mL(r=0.9999),5.2-15.5 μg/mL(r=0.9998)and 31.5-94.5 μg/mL(r=0.9994),respectively. The limits of quantitation were 2.442,0.010,2.520 μg/mL,and the limits of detection were 0.810,0.003, 0.830 μg/mL,respectively. RSDs of precision,stability and reproducibility tests were lower than 1.0%. The recoveries of them were 99.2%-101.7%(RSD=0.9%,n=9),96.4%-102.0%%(RSD=1.7%,n=9),100.2%-101.9%(RSD=0.4%,n=9),respec-tively. CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for simultaneous determination of ephedrine hydrochloride,nitrofural and diphenhydramine hydrochloride in Compound diphenhydramine nasal drop.
ABSTRACT
OBJECTIVE: To establish a method for the simultaneous determination of the contents of salicylic acid and nitrofural in Fu-La pastes.METHODS: A dual-wavelength spectrophotometry was adopted for the determination of the contents of salicylic acid and nitrofural with water used as solvent.The content of salicylic acid was determined with the detective wavelength set at 296.6 nm and the reference wavelength set at 314.4 nm.The content of the nitrofural was determined directly with the detective wavelength set at 375.0 nm.RESULTS: The linear ranges of salicylic acid and nitrofural were 10.77～43.08 ?g?mL-1(r=0.999 0) and 2.58～10.32 ?g?mL-1(r=1.000 0),respectively;and their average recovery rates were 99.55%(RSD=0.47%) and 100.03%(RSD=0.80%),respectively.CONCLUSION: The method is simple,rapid and accurate,and suitable for the quality control of Fu-La pastes.