ABSTRACT
Transposition of the great arteries(TGA) is one of the most common and severely congenital heart defects in the neonatal period,even though we have significantly improvement in prenatal diagnosis and postpartum treatment of TGA,patients with severely TGA still have no promising prognosis.Now,the exactly pathogenesis of TGA is still unknown,in the view of embryonic development,TGA is a kind of conotruncal anomalies,which highly associated with unbalanced development of left-right axis.Nodal signaling pathway is one of the most functional pathway in controlling the formation of left-right axis,so fully investigation of Nodal signaling pathway may help us to find deep pathogenesis of TGA.
ABSTRACT
Objective To investigate the expression of Nodal and its receptors in different tissues and organs of different development stages of mice.Methods Ten pairs(male and female each for a pair) of mice were divided into the four groups:3 pairs served as adult mice group,the rest 7 pairs were allowed to mate,among them 3 pregnant mice served as the fetal group,and 3 pregnant mice served as the neonatal group and 1 pregnant mouse served as filial group.The multiple tissues and organs such as brain,liver,kidney,heart,lung were selected from fetal,neonatal,filial and adult mice for preparing the protein samples.Western blot was performed to detect the expression of Nodal and its type Ⅰ receptors of ALK7 and ALK4 as well as auxiliary receptor Cripto-1.Results Only cerebrum,cerebellum,liver and kidney had Nodal express in the four different mouse ontogenetic stages,in which only liver and kidney simultaneously expressed Nodal and its receptor protein in the whole four ontogenetic stages.Besides,most tissues and organs of adult mice expressed Nodal and its receptor protein,which was significantly different from the fetal,neonatal and filial mice.Conclusion Nodal signaling might have a certain effect on the growth and development of mouse liver and kidney during the late development stage.