Relationship between esophageal visceral hyperesthesia and expression of substance P in LES of patients with non-erosive gastro-oesophageal reflux disease / 解放军医学杂志

Objective To investigate the correlations between esophageal visceral hyperesthesia and the expression of substance P (SP) immunoreactive fibers in the lower esophageal sphincter (LES). Methods Esophageal sensitivity to mechanical stimuli was measured by esophageal barostat. The Bernstein acid infusion test was used to evaluate the esophageal sensitivity to intraesophageal perfusion of 0.1mol/L HCl .The distribution and expression of SP positive fibers within mucosal layer of LES were detected by immonohistochemical staining techniques. Results In comparison with control group, the initial perception threshold and maximally tolerated pain threshold of NERD patients were significantly reduced (P

Cerebral evoked potentials response to esophageal mucosal acid exposure and distention in patients with non-erosive gastro-oesophageal reflux disease / 中华消化杂志

Objective To investigate characteristics and alternation of cerebral evoked potentials (CEP) response to esophageal mucosal acid exposure and distention in patients with non-erosive gastro-oesoph- ageal reflux disease (NERD) and in healthy subjects,and to study the mechanism of visceral hypersensitivity in NERD.Methods Twenty-one NERD patients and 10 volunteers were recruited.Mechanical distention stimulation and acid perfusion of the esophagus were performed using the balloon-affixed and polyvinyl multi- lumen catheter.First,maximally tolerated pain thresholds of all subjects were recorded,then esophageal mechanical stimulation with a 75% of maximal tolerated intensity and a frequency of 0.2 Hz was performed altogether 64 times by means of a computer-controlled barostat.The alternation of esophageal CEP was recorded before and after acid perfusion with a multichannel international 10-20 system of electroencephalography. Experimental data was analyzed by student's t-test and one way analysis of variance.Results Esophageal mu- cosal distention may evoke recognizable and reproducible and multi-peak CEP.The latencies for N1,P1 and N2 in volunteers were (246?77),(388?84)and (502?78) ms,CEP morphology of NERD patients was charac- terized by randomly distributed patterns,and the latencies for N1 ,P1 and N2 were (192?46),(293?76) and (440?79)ms,significantly shorter for mechanical stimulation compared with those of control group respectively (all P value