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OBJECTIVE@#To evaluate the effectiveness and safety of Chinese herbal external umbilicus treatment with Modified Dinggui Powder (, MDGP) in patients with chronic nonbacterial prostatitis (CNP).@*METHODS@#A randomized, double-blind, placebo-controlled clinical trial was conducted among 72 patients with CNP. Participants were randomly allocated to a treatment group and a placebo group using computer software in a 1:1 ratio, and received either MDGP external umbilicus treatment (MDGP group, 36 cases) or placebo control groupl (36 cases) at acupoints Shenque (CV 8), twice a week for 4 weeks. In addtion, patients all received herbal medicine treatment twice a day for 4 weeks. The primary outcomes was the US National Institutes of Health Chronic Prostatitis Symptom Scores Index (NIH-CPSI) with a questionnaire at weeks 2 and 4. The secondary outcomes including prostatic fluid examination (white blood cells and lecithin bodies), the clinical efficacy evaluation, and the adverse events were also assessed during the entire trial.@*RESULTS@#The NIH-CPSI scores regarding pain or discomfort scores showed greater improvement in the MDGP group than placebo control group at weeks 2 (P0.001) and week 4 (P0.004), respectively. NIH-CPSI scores of symptom severity, total scores, the amount of leukocytes number in the prostatic fifluid in the MDGP group were significantly improved (P0.05). The clinical effective rate was 73.53% (25/34) in the MDGP group, which was significally higher than the placebo control group with 48.39% (25/31, P<0.05). Patients were blinded successfully, and no serious adverse effects were found during the trial.@*CONCLUSION@#A 4-week course of umbilicus treatment with modified Dinggui Powder seems to relieve pain and symptom severity effectively and increase the amount of leukocytes number in patients with CNP (Trial registration No. ChiCTR1800014687).
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OBJECTIVE: To observe the clinical effect of acupuncture stimulation of Sanhuang points in the treatment of chronic nonbacterial prostatitis (CNP) of kidney-yang deficiency type. METHODS: A total of 60 CNP outpatients were equally randomized into medication(control) group and acupuncture plus medication (acupuncture) group. Patients of the control group were ordered to take Tamsulosin (0.2 mg/d) for successive 8 weeks. On the basis of medication treatment, for patients of the acupuncture group, bilateral Sanhuang points including Tianhuang-fu Point (Shenguan), Dihuang Point and Renhuang Point (at the lower leg) were needled with filiform needles which were manipulated for a while till Deqi, followed by retaining the needles for 30 min. The treatment was conducted once daily for successive 8 weeks. The therapeutic effect was evaluated in reference to the "Criteria for Diagnosis and Therapeutic Effect Evaluation of Syndromes/Diseases of Traditional Chinese Medicine". The National Institutes of Health-Chronic Prostatitis Symptom Index (NIH-CPSI) scores were used to assess the severity of chronic prostatitis. Routine examination of the leukocyte count in the prostatic fluid was performed before and after the treatment. RESULTS: Of the two 30 cases of the control and acupuncture groups, 9 and 19 were cured, 14 and 10 experienced improvement, and 7 and 1 failed in the treatment, with the effective rates being 76.67% and 96.67%, respectively. The effective rate of the acupuncture plus medication was significantly higher than that of the medication (P<0.05). After the treatment, the scores of NIH-CPSI and white blood cell counts in the prostatic fluid in both groups were significantly decreased in comparison with their own pre-treatment (P<0.05). The therapeutic effect of acupuncture plus medication was significantly superior to that of the medication alone in reducing the NIH-CPSI symptom score and the number of leukocytes in the prostatic fluid (P<0.05). CONCLUSION: Acupuncture at Sanhuang points is effective in improving symptoms of CNP patients of kidney-yang deficiency type, and the therapeutic effect of acupuncture plus medication is superior to that of medication alone.
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MicroRNAs (miRNAs) are considered to be involved in the pathogenic initiation and progression of chronic nonbacterial prostatitis (CNP); however, the comprehensive expression profile of dysregulated miRNAs, relevant signaling pathways, and core machineries in CNP have not been fully elucidated. In the current research, CNP rat models were established through the intraprostatic injection of carrageenan into the prostate. Then, next-generation sequencing was performed to explore the miRNA expression profile in CNP. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) bioinformatical analyses were conducted to reveal the enriched biological processes, molecular functions, and cellular components and signaling pathways. As a result, 1224, 1039, and 1029 known miRNAs were annotated in prostate tissues from the blank control (BC), normal saline injection (NS), and carrageenan injection (CAR) groups (n = 3 for each group), respectively. Among them, 84 miRNAs (CAR vs BC) and 70 miRNAs (CAR vs NS) with significantly different expression levels were identified. Compared with previously reported miRNAs with altered expression in various inflammatory diseases, the majority of deregulated miRNAs in CNP, such as miR-146b-5p, miR-155-5p, miR-150-5p, and miR-139-5p, showed similar expression patterns. Moreover, bioinformatics analyses have enriched mitogen-activated protein kinase (MAPK), cyclic adenosine monophosphate (cAMP), endocytosis, mammalian target of rapamycin (mTOR), and forkhead box O (FoxO) signaling pathways. These pathways were all involved in immune response, which indicates the critical regulatory role of the immune system in CNP initiation and progression. Our investigation has presented a global view of the differentially expressed miRNAs and potential regulatory networks containing their target genes, which may be helpful for identifying the novel mechanisms of miRNAs in immune regulation and effective target-specific theragnosis for CNP.
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MicroRNAs (miRNAs) are considered to be involved in the pathogenic initiation and progression of chronic nonbacterial prostatitis (CNP); however, the comprehensive expression profile of dysregulated miRNAs, relevant signaling pathways, and core machineries in CNP have not been fully elucidated. In the current research, CNP rat models were established through the intraprostatic injection of carrageenan into the prostate. Then, next-generation sequencing was performed to explore the miRNA expression profile in CNP. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) bioinformatical analyses were conducted to reveal the enriched biological processes, molecular functions, and cellular components and signaling pathways. As a result, 1224, 1039, and 1029 known miRNAs were annotated in prostate tissues from the blank control (BC), normal saline injection (NS), and carrageenan injection (CAR) groups (n = 3 for each group), respectively. Among them, 84 miRNAs (CAR vs BC) and 70 miRNAs (CAR vs NS) with significantly different expression levels were identified. Compared with previously reported miRNAs with altered expression in various inflammatory diseases, the majority of deregulated miRNAs in CNP, such as miR-146b-5p, miR-155-5p, miR-150-5p, and miR-139-5p, showed similar expression patterns. Moreover, bioinformatics analyses have enriched mitogen-activated protein kinase (MAPK), cyclic adenosine monophosphate (cAMP), endocytosis, mammalian target of rapamycin (mTOR), and forkhead box O (FoxO) signaling pathways. These pathways were all involved in immune response, which indicates the critical regulatory role of the immune system in CNP initiation and progression. Our investigation has presented a global view of the differentially expressed miRNAs and potential regulatory networks containing their target genes, which may be helpful for identifying the novel mechanisms of miRNAs in immune regulation and effective target-specific theragnosis for CNP.
Subject(s)
Animals , Male , Rats , Computational Biology , Databases, Genetic , Gene Expression Profiling , High-Throughput Nucleotide Sequencing , MicroRNAs/metabolism , Prostate/metabolism , Prostatitis/metabolism , Rats, WistarABSTRACT
Objective To establish an animal model of chronic nonbacterial prostatitis induced by chemical sub-stances, and provide a reliable animal model and evaluation method for pathological mechanism and pharmaceutical re-search of chronic nonbacterial prostatitis. Methods SD male rats were randomly divided into control group and model groups A, B and C. The three model groups were respectively treated with 20, 50 and 100 μL of 1% sterile carrageenan, injected into the left and right ventral lobes of rat prostate. The control group was injected 50μL sterile normal saline. The rats were sacrificed at 7 days after carrageenan injection, and the anatomical changes were analyzed, and the prostate in-dex, leukocyte count, the histology of prostate, and the protein expressions of TNF-α, NF-κB, IKKα, p-IKB-α and COX-2 were analyzed. Results Compared with the sham operation group, the softness of prostate tissue of groups A, B and C was decreased, the elasticity of the prostatic tissue was weakened, and the prostate tissues of model groups were ad-hered to surrounding tissues. The total number of leukocytes and the prostate index of model groups A, B and C were signif-icantly increased (P<0. 01), by 21. 1%, 61. 7% and 72. 7%, respectively. The total increase rate of white blood cell in the model groups A, B and C was 75%, 103. 6% and 114. 8%, respectively. Pathological examination showed that the in-terstitial edema of the prostate of model group A was minimal, but obvious in the groups B and C. Moreover, in the group C, the prostate atrophy was obvious, and some of the glands were degenerated and necrotic. The protein expression levels of NF-κB, IKKα, p-IKB-α, TNF-α, and COX-2 in the prostate tissue were increased to a different extent in all model groups. Conclusions Inflammatory reactions can be induced by injecting 20, 50 or 100 μL of 1% sterile carrag-eenan into the right and left ventral lobes of the rat prostate. However, the 20μL dose is too small, inducing only weak in-flammatory response, with considerable operation error, while the dose of 100μL induced excessive inflammatory response, even rat death. The dose of of 50 μL injection is most suitable to establish rat models of nonbacterial prostatitis, showing apparent activation of NF-κB inflammatory signaling pathway.
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Objective To establish an animal model of chronic nonbacterial prostatitis induced by chemical sub-stances, and provide a reliable animal model and evaluation method for pathological mechanism and pharmaceutical re-search of chronic nonbacterial prostatitis. Methods SD male rats were randomly divided into control group and model groups A, B and C. The three model groups were respectively treated with 20, 50 and 100 μL of 1% sterile carrageenan, injected into the left and right ventral lobes of rat prostate. The control group was injected 50μL sterile normal saline. The rats were sacrificed at 7 days after carrageenan injection, and the anatomical changes were analyzed, and the prostate in-dex, leukocyte count, the histology of prostate, and the protein expressions of TNF-α, NF-κB, IKKα, p-IKB-α and COX-2 were analyzed. Results Compared with the sham operation group, the softness of prostate tissue of groups A, B and C was decreased, the elasticity of the prostatic tissue was weakened, and the prostate tissues of model groups were ad-hered to surrounding tissues. The total number of leukocytes and the prostate index of model groups A, B and C were signif-icantly increased (P<0. 01), by 21. 1%, 61. 7% and 72. 7%, respectively. The total increase rate of white blood cell in the model groups A, B and C was 75%, 103. 6% and 114. 8%, respectively. Pathological examination showed that the in-terstitial edema of the prostate of model group A was minimal, but obvious in the groups B and C. Moreover, in the group C, the prostate atrophy was obvious, and some of the glands were degenerated and necrotic. The protein expression levels of NF-κB, IKKα, p-IKB-α, TNF-α, and COX-2 in the prostate tissue were increased to a different extent in all model groups. Conclusions Inflammatory reactions can be induced by injecting 20, 50 or 100 μL of 1% sterile carrag-eenan into the right and left ventral lobes of the rat prostate. However, the 20μL dose is too small, inducing only weak in-flammatory response, with considerable operation error, while the dose of 100μL induced excessive inflammatory response, even rat death. The dose of of 50 μL injection is most suitable to establish rat models of nonbacterial prostatitis, showing apparent activation of NF-κB inflammatory signaling pathway.
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<p><b>OBJECTIVE</b>To observe the clinical effects differences and partial mechanism for chronic nonbacterial prostatitis (CNP) among drug oil moxibustion, simple moxibustion, and conventional western medicine.</p><p><b>METHODS</b>A total of 120 patients who met the criteria of inclusion were randomly assigned into a drug oil moxibustion group, a moxibustion group and a western medication group, 40 cases in each one. Moxibustion was used at Guanyuan (CV 4), Zhongji (CV 3), Qihai (CV 6) and bilateral Yinlingquan (SP 9), Sanyinjiao (SP 6), Shenshu (BL 23), Mingmen (GV 4), Pangguangshu (BL 28), Ciliao (BL 32), and Zhibian (BL 54), etc. The same moxibustion was used at the same acupoints in the drug oil moxibustion group after external application of medicated oil. Thirty min treatment was used once a day in alternated abdomen and back. In the western medication group, oral tamsulosin hydrochloride capsules were applied once a day, one capsule at a time. All the treatment was given for 30 days. Chronic prostatitis symptom index from National Institutes for Health (NIH-CPSI), the contents of Zinc (Zn) and C-reactive protein (CRP), as well as the number of white blood cells (WBC) and density of lecithin bodies were observed before and after treatment and 1 month after treatment. The effects were evaluated after treatment.</p><p><b>RESULTS</b>After treatment, the total effective rate of the drug oil moxibustion group was 90.0% (36/40), which was significantly higher than 72.5% (29/40) of the moxibustion group and 62.5% (25/40) of the western medication group (both<0.05). After treatment and at follow-up in the three groups, the NIH-CPSI scores were lower than those before treatment (all<0.05), and those in the drug oil moxibustion group were lower than the results in the moxibustion group and the western medication group (all<0.05). The contents of Zn in the three groups were higher than those before treatment (all<0.05), with better results in the drug oil moxibustion group (all<0.05), and higher Zn contents in the moxibustion group compared with those in the western medication group (both<0.05). The CRP levels were lower than those before treatment (all<0.05), and those in the drug oil moxibustion group were better than those in the moxibustion group and western medication group (all<0.05). The CRP contents in the moxibustion group were lower than those in the western medication group (both<0.05). The number of WBC were lower than those before treatment (all<0.05), with better results in the drug oil moxibustion group (all<0.05). The concentrations of lecithin were higher than those before treatment (all<0.05), with better results in the drug oil moxibustion group (all<0.05).</p><p><b>CONCLUSIONS</b>The clinical effect of drug oil moxibustion is better than those of simple moxibustion and western medicine, which has advantages in improving clinical symptoms, Zn, the density of lecithin body and decreasing CRP content and the number of WBC.</p>
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<p><b>Objective</b>To investigate the expressions of substance P (SP) and neurokinin-1 receptor (NK-1R) in the posterior horn of the L5－S2 spinal cord in the rat model of chronic nonbacterial prostatitis (CNP) at different time points of modeling.</p><p><b>METHODS</b>Forty adult male SD rats were randomly divided into four groups of equal number, control, 45 d model, 60 d model, and 90 d model, and proteins were obtained from the prostatic tissue of another 30 rats. The CNP model was made by intraperitoneal injection of 0.5 ml DPT vaccineand intradermal injection of mixed solution of 1 ml prostatein extract and complete adjuvant at a 1∶1 ratio, while the control rats were injected with the same volume of normal saline. At 45, 60, and 90 days after modeling, we measured the paw withdrawal threshold (PWT) of the rats, determined the levels of TNF-α, IL-1β, IL-2, and IL-10 in the prostate tissue by ELISA, observed the histomorphological changes in the prostate by transmission electron and light microscopy, and detected the expressions of SP and NK1-R in the L5－S2 spinal cord by immunohistochemistry.</p><p><b>RESULTS</b>The model rats showed significantly increased sensitivity to pain, with remarkably lowered PWT at 45, 60, and 90 days after modeling. The levels of TNF-α, IL-1β, IL-2, and IL-10 in the prostate tissue were markedly elevated in the CNP models as compared with those in the controls (all P<0.05), most significantly at 90 days (all P<0.05). Immunohistochemistry showed that the expressions of SP and NK-1R were remarkably higher in the CNP model groups than in the control (all P<0.05), the highest at 90 days. Light microscopy revealed no inflammatory cell infiltration in the prostate tissue of the control rats, and obvious edema and increased lymphocytes were observed with the prolonged time of modeling.Transmission electron microscopy showed inflammatory changes in the prostate tissue of the model rats and that peritubular interstitial edema was most obvious at 90 days, with widened intervals between peritubular cells and the epithelial base and increased numbers of fibroblasts and collagen fibrils.</p><p><b>CONCLUSIONS</b>The synthesis of SP and the level of NK-1R were increased in the posterior horn of the L5－S2 spinal cord in the rat model of CNP.</p>
Subject(s)
Animals , Male , Rats , Interleukin-10 , Metabolism , Interleukin-1beta , Metabolism , Interleukin-2 , Metabolism , Pain , Prostatitis , Metabolism , Random Allocation , Rats, Sprague-Dawley , Receptors, Neurokinin-1 , Metabolism , Spinal Cord , Metabolism , Substance P , Metabolism , Tumor Necrosis Factor-alpha , MetabolismABSTRACT
OBJECTIVE:To study the effects of total glucosides of Verbena officinalis on chronic nonbacterial prostatitis (CNP)in mice and its anti-inflammatory and analgesic effects. METHODS:60 mice were randomized into normal group(normal saline),model group(normal saline),positive group(Qianliekang tablet 1.5 g/kg)and total glucosides of V. officinalis high-dose, medium-dose and low-dose groups(0.2,0.1,0.05 g/kg),with 10 mice in each group. CNP model was induced in those groups ex-cept for normal group;mice were given relevant medicine once a day for consecutive 21 days since 8th day of modeling. The white blood cell count,lecithin corpuscle density and pathological changes of prostate samples were observed. 50 mice were randomly di-vided into model group(normal saline),positive group(Aspirin tablet 0.3 g/kg or Dexamethasone acetate tablet 0.01 g/kg)and to-tal glucosides of V. officinalis high-dose,medium-dose and low-dose groups(0.2,0.1,0.05 g/kg);they were given relevant medi-cine intragastrically once a day for consecutive 7 days. The xylene-induced ear swelling test (Aspirin tablet as positive drug) and cotton-pellet granuloma test (Dexamethasone acetate tablet as positive drug) were conducted to determine the the degree of ear swelling and the net weight of granuloma. The acetic acid writhing test and hot plate test(Aspirin tablet as positive drug)were car-ried out to determine the latency period of writhing,the times of writhing within 20 min and pain threshold. RESULTS:Compared with model group,white blood cells count of prostate tissue were lowered while the density of lecithin corpuscles were increased significantly in medicine groups;the pathology of prostate tissue were improved significantly in positive group and total glucosides of V. officinalis high-dose and medium-dose groups,and mostly were 0 and Ⅰ grade. The degree of ear swelling and the net weight of granuloma were markedly reduced in positive group and total glucosides of V. officinalis high-dose and medium-dose groups;the times of writhing within 20 min decreased in medicine groups,and the latency period of writhing prolonged and pain threshold in-creased in positive group and total glucosides of V. officinalis high-dose group. All of the results showed statistical difference(P<0.05 or P<0.01). CONCLUSIONS:Total glucosides of V. officinalis can improve CNP in mice,and shows good anti-inflammatory and analgesic effects.
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Objective To investigate the clinical efficacy of Qingzhuo Qudu Pills combined with acupuncture in treating chronic nonbacterial prostatitis and to explore the possible molecular mechanism. Methods A total of 160 cases of chronic nonbacterial prostatitis were randomly divided into western medicine group, Chinese medicine group, acupuncture group and combined group, 40 cases in each group. The western medicine group was given Tamsulosin and Celecoxib tablets orally, Chinese medicine group was given Qingzhuo Qudu Pills, acupuncture group were given acupuncture, and the combined group were given Qingzhuo Qudu Pills plus acupuncture. Treatment period covered 30 days. Before and after treatment, the clinical efficiency of the four groups was assessed by the US National Institutes of Health chronic prostatitis symptom index ( NIH-CPSI) scores, and routine prostatic fluid examiantion and drug safety assessment were also carried out. Results ( 1) The total effective rate was 69.23%, 84.62%, 74.36% and 92.50% in the western medicine group, Chinese medicine group, acupuncture group and combined group, respectively; the combined group had the best clinical efficiency ( P0.05). (2) After treatment, the total scores and scores of each item of NIH-CPSI were decreased in the four groups ( P<0.05 compared with those before treatment) , and the improvement of general symptoms, the quality of life, pain and urination in the combined group was superior to that in the other 3 groups ( P<0.05) . ( 3) After treatment, levels of tumor necrosis factor alpha (TNF-α) and interleukin 1 beta (IL-1β) were decreased to various degrees (P<0.05 or P<0.01 compared with those before treatment), and the decrease in the combined group was superior to that in the other 3 groups (P<0.05). (4) The blood, urine and stool routine examination, electrocardiography, hepatic and renal function of the 4 groups stayed normal before and after treatment, and no adverse reaction was present in the 160 cases. Conclusion Qingzhuo Qudu Pills combined with acupuncture is safe and effective in treating chronic non-bacterial prostatitis through relieving symptoms and reducing the degrees of prostatitis. Cytokines may play an important role in the pathogenesis and treatment of chronic prostatitis.
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Objective To establish a rat model of chronic nonbacterial prostatitis (CNP) and investigate the histopathological characteristics of CNP in rat. Methods The prostate tissues of 4 male SD rats were taken out under aseptic condition,and prostate tissue homogenate supernate (PTHS) was made.Another 20 male SD rats were randomly divided into control group and CNP group with 10 rats each.Each rat of group CNP was immunized with 1.0 ml PTHS (20 mg/ml) emulsified by isopyknic Freund's complete adjuvant (FCA) intradermally in the multiple points,and simultaneously immunized with 0.5 ml pertussisdiphtheria-tetanus (PDT) vaccine intraperitoneally on 0 and 30th day.Each rat of control group was injected with equivalent normal saline in the same way.Rats from each group were sacrificed 45 days after immunization,and the prostates were harvested under aseptic condition.Prostatic tissues were examined macropathologically and histologically for degree of inflammation.Another 80 CNP rats were established after confirming success of the modeling.They were divided into high-dose androgen group (H),medial-dose androgen group (M),low-dose androgen group (L) and control group (C) with 20 in each.Rats of group H,M and L were injected with different concentrations of testosterone propionate ( 1,2,4 mg/ml).Rats of group C were injected with sterilized peanut oil.O.5 ml was applied through subcutaneous injection every other day.Each group was equally divided into 4 subgroups,in which the durations of medication were 1,2,4 and 6 weeks,respectively.After the expiration of the injection,animals from each subgroup were sacrificed and the macropathological and histological features of the prostatic tissues were examined as above. Results Macroscopic features of prostate tissues of group CNP at the 45th day were serious congestion and edema,adhesion with surrounding tissues,aneretic prostate capsule and so on.Histologically,the prostatic tissues were characterized by lymphoid tissue proliferation and chronic inflammatory cell infiltration in the stromal connective tissue around the acini or ducts.The rats of control group showed no inflammatory manifestations as above.After injection of testosterone propionate,the inflammatory degree of the CNP rats was lessen in varying degrees.For example,the destructive glands and stroma appeared repair and regeneration,the lymphoid tissue proliferation was alleviated,and the locations,ranges and amount of chronic inflammatory cell infiltration were also decreased.Furthermore,the relief of inflammatory degree had a positive correlation with the concentration and duration of testosterone propionate.The degree of inflammation in group C scarcely changed. Finally,the histopathological characteristics of CNP in rat model were summarized according to the results of histopathology,including inflammation locations,ranges and grades.Inflammation locations:①inflammation in the glands referred to the inflammatory cell infiltration in the ductal epithelium,alveolar epithelial and (or)glandular cavity; ②inflammation in the periglandular tissues referred to the inflammatory cell infiltration in the stroma and around the glandulartube; ③inflammation in the stroma referred to the inflammatory cell infiltration in the stroma without the glandulartube.Inflammation-rangeg:①focal inflammation indicates that the area of inflammatory cell infiltration was less than 10％ ; ②multifocal inflammation indicates that the area of inflammatory cell infiltration ranged from 10％ to 50％ ; ③diffuse inflammation indicates that the area of inflammatory cell infiltration was more than 50％.Inflammation grades:①grade Ⅰ meant that scattered inflammatory cells were in the specific area,cell count 1 - 10/HP; ②grade Ⅱ meant that inflammatory cell assembled without glandular epithelial tissue destruction or lymphoid nodule/follicle formation,cell count l1 -20/HP; ③grade Ⅲ meant that inflammatory cell assembled with part of glandular epithelial tissue destruction or lymphoid nodule/follicle formation,cell count ＞ 20/HP; ④grade Ⅳ meant that inflammatory cell assembled with a mass of glandular epithelial tissue destruction or lymphoid nodule/follicle formation,cell count full field of vision/HP. Conclusions The CNP rat model can be established by immunized with syngeneic PTHS plus FCA and PDT.The histopathological characteristics of CNP in rat can be evaluated according to the locations,ranges and grades of inflammation,which serve for further research of pathogenesis and treatment of this disease.
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PURPOSE: Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS, NIH category III) accounts for 90-95% of prostatitis cases. However, standard treatment has not yet been established. It is known that polyphenols have an inhibitory effect on inflammation by their antioxidative capacity, and oligonol, a polyphenol derivative, has much higher bioavailability and bioactivity than common polyphenols. We investigated the anti-inflammatory effects and mechanisms of oligonol in estradiol-induced prostatitis rat models. MATERIALS AND METHODS: Prostatitis was induced by 17 beta-estradiol (E2) and dihydrotestosterone (DHT) in Wistar male rats (n = 20). Ten rats were placed in the oligonol-treated group and 10 in the E2 + DHT-treated group. The other 10 rats were also included as normal control group. Oligonol (60 mg/kg/day) was administered via gavage tube for 4 weeks. Superoxide dismutase (SOD), glutathione peroxidase (GPx), and tumor necrosis factor-alpha (TNF-alpha) were quantified, and phosphorylation of IkappaBa and histological changes were also evaluated in prostatic tissue. RESULTS: The SOD and GPx activity showed tendencies to increase in the oligonol-treated group compared to the normal control group. TNF-alpha expression was slightly reduced in the oligonol-treated group. Western blotting demonstrated that phosphorylation of IkappaBa in the oligonol-treated group was significantly lower than in the normal control group. The E2 + DHT-treated group revealed severe atrophy of acinar epithelial cells and infiltration of leukocytes and lymphocytes in the prostate, however, the oligonol-treated group showed overall reduction in inflammatory features. CONCLUSION: This study demonstrates that oligonol improves estradiol-induced non-bacterial prostatitis by regulating phosphorylation of IkappaBa. These findings suggest that oligonol has a beneficial effect on prevention and treatment of CP/CPPS.
Subject(s)
Animals , Male , Rats , Blotting, Western , Body Weight/drug effects , Estradiol/adverse effects , Flavonoids/therapeutic use , Immunoassay , Phenols/therapeutic use , Prostate/drug effects , Prostatitis/chemically induced , Rats, Wistar , Superoxide Dismutase/metabolism , Tumor Necrosis Factor-alpha/metabolismABSTRACT
OBJECTIVE:To study the action mechanism of the self-made Wuhuang tang(Chinese medicine)in the treatment of type Ⅲ prostatitis(chronic nonbacterial prostatitis,CNBP)so as to promote the action mechanism of traditional Chinese medicine to the molecular level.METHODS:90 patients with CNBP were divided into two groups(n=45 each):one group received western medicine alone,another group received integrated Chinese and western medicine(ICWM)(Wuhuangtang plus western medicine).The indicators such as the expressed prostatic secretions(EPS)routine,IL-1?,and TNF-? before and after the treatment were analyzed statistically.RESULTS:The levels of IL-1? and TNF-? in ICWM-treated group decreased significantly and the clinical symptoms of these patients improved significantly as compared with those treated with western medicine alone.CONCLUSION:The self-made Wuhuang tang can markedly alleviate the symptoms of CNBP patients and decrease levels of IL-1? and TNF-? in patients' EPS,thus it is an effective clinical empirical prescription for CNBP.
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PURPOSE: The etiology of chronic nonbacterial prostatitis is unclear but it appears to be either an infectious disease caused by yet unidentified pathogens or a noninfectious form of inflammation. But, recent data showed that the chronic nonbacterial prostatitis is developed from abnormalities in autoimmunity. We examined the serum CD4, CD8, CD4/CD8 and CD16 levels in patients with chronic nonbacterial prostatitis and normal volunteers, so we tried to reveal their differences in both two groups and the relationship of its pathogenesis. MATERIALS AND METHODS: Between Aug. 1996 and Nov. 1996, we examined the serum level of CD4, CD8 and CD16 in patient with chronic nonbacterial prostatitis(n=20) and in normal patients including volunteers(n=16). RESULTS: The results showed decreased CD4, and increased CD8 which lead to decreased CD4/CD8 ratio and normal CD16 levels. CONCLUSIONS: We believe that our experimental data would show changes of T-cell subclones in chronic nonbacterial prostatitis patients and suggest the basic data in understanding the etiology of chronic nonbacterial prostatitis.
Subject(s)
Humans , Autoimmunity , Communicable Diseases , Healthy Volunteers , Inflammation , Prostatitis , T-LymphocytesABSTRACT
The results of intraprostatic injection of fosfomycin were compared to those of oral minocycline therapy in patients with nonbacterial prostatitis. Nonbacterial prostatitis did not recur in 26 out of 33 patients with fosfomycin injected into the prostate and in 16 out of 48 patients treated with oral minocycline. Non-recurrence rate within each group was compared as 78.8 per cent to 33.3 per cent, respectively, demonstrating that intraprostatic fosfomycin treatment was more successful than oral minocycline therapy. The pain and discomfort experienced by the patients during direct injection into the prostate was minimal. Hematuria was always practically present after the injection. Intraprostatic fosfomycin injection is believed to be new alternative method in the treatment of nonbacterial prostatitis.