ABSTRACT
Objective: To observe the remodeling of diabetic microvessels in diabetic rats. Methods: Diabetic animal models were induced by STZ on SD rats. The rats were treated with nonenzymatic glycosylation inhibitor and blood flow activating and stasis removing traditional Chinese medicine to evaluate the remodeling of diabetic retinal microvessels by trypsin digestion and figure analysis. Results: At 8 weeks, significant alteration of retinal capillary width in diabetic rats were observed( P
ABSTRACT
A simplified colorimetric method for measurement of the levels of glycosylation of proteins was developed by a modification of an existing method. Employing this method, the extent of nonenzymatic glycosylation of apolipoprotein B subspecies(B-100, B-74, B-26), LDL, VLDL and total serum proteins in human plasma obtained from patients with diabetes mellitus and control subjects was compared. Plasma LDL (1.019 < d < 1.063) and VLDL(d < 1.006) were separated using the sequential ultracentrifugation method, and the subspecies of apolipoprotein B were isolated by extracting them from polyacrylamide gels after they were separated by preparative SDS-polyacrylamide gel electrophoresis. Increases in the level of glycosylation of serum proteins, LDL, VLDL, and apo B subspecies obtained from diabetic patients were observed. Among them, the increases of glycosylated LDL and apo B-26 were most significant (p < .001). Also, good correlations were found between glycosylations of apo B-26 and LDL (r=.88), and glycosylation of LDL and LDL cholesterol level(r=.79). The results also showed an excellent correlation between levels of HbA1c and glycosylated apo B-26(r=.93).
Subject(s)
Adult , Humans , Colorimetry/methods , Diabetes Mellitus, Type 2/blood , Glycosylation , Lipoproteins, LDL/bloodABSTRACT
Aortic collagen advanced glycosylation endproduct (AGE) and collagen contents were determined in 8 streptozotocin-induced diabetic rats and 8 control rats. Ten weeks after a peritoneal injection of streplozotocin, the AGE and collagen contents were significantly increased in diabetic rats (P