ABSTRACT
We report a case of microgranular variant of acute promyelocytic leukemia with strong positivity in nonspecific esterase stain (inhibited by sodium fluoride) in a 21-onth-ld girl who had complained of intermittent fever and severe vomiting. At admission, she showed marked leukocytosis in the peripheral blood. The majority of leukocytes were immature cells which exhibited marked nuclear irregularity and had sparse dust-ike granules in their cytoplasm. In immunophenotyping, these cells showed CD13 and CD33 positivity and in cytogenetic study, they showed t(15;17) abnormality. The patient died at the 6th hospital day because of pulmonary and intracranial hemorrhage.
Subject(s)
Female , Humans , Carboxylesterase , Cytogenetics , Cytoplasm , Fever , Immunophenotyping , Intracranial Hemorrhages , Leukemia, Promyelocytic, Acute , Leukocytes , Leukocytosis , Sodium , VomitingABSTRACT
We report a case of microgranular variant of acute promyelocytic leukemia with strong positivity in nonspecific esterase stain (inhibited by sodium fluoride) in a 21-onth-ld girl who had complained of intermittent fever and severe vomiting. At admission, she showed marked leukocytosis in the peripheral blood. The majority of leukocytes were immature cells which exhibited marked nuclear irregularity and had sparse dust-ike granules in their cytoplasm. In immunophenotyping, these cells showed CD13 and CD33 positivity and in cytogenetic study, they showed t(15;17) abnormality. The patient died at the 6th hospital day because of pulmonary and intracranial hemorrhage.
Subject(s)
Female , Humans , Carboxylesterase , Cytogenetics , Cytoplasm , Fever , Immunophenotyping , Intracranial Hemorrhages , Leukemia, Promyelocytic, Acute , Leukocytes , Leukocytosis , Sodium , VomitingABSTRACT
The met-enkephalin (MEK), leu-enkephalin (LEK) and ?-endorphin (?-EP) immunoreactivities were detected in the circulating lymphocytes of 64 guinea pigs by PAP immunohistochemical method under the conditions of electroacupuncture, naloxone or exogenous opioid like peptides (OLP). Subsequently the ?-naphthyl acetate esterase (ANAE)was demonstrated on partial immunostained lymphocyte samples. The results showed that the OLP immunoreactivity with different intensity was found in approximate 96％ of the lymphocytes. There was a positive correlation between the ANAE-focal pattern lymphocytes and MEK-IIRL (intense immunoreactivity lymphocytes)or ?-EP-IIRL; whereas there was no significant correlation between the ANAE-focal pattern lymphocytes and LEK-IIRL. The MEK-IIRL, LEK-IIRL and ?-EP-IIRL were all increased markedly in the guinea pigs which underwent electroacupuncture at "Zusanli" points. There was no significant difference in MEK-IIRL, LEK-IIRL and ?-EP-IIRL, when the acupuncture group, naloxone group and control group were compared with each other. However, the LEK-IIRL, MEK-IIRL and ?-EP-IIRL could be depressed by naloxone, when the lymphocytes were preincubated with 10~(-7)mol/L of MEK, LEK or ?-EP in vitro. Hence, the naloxone group had a significant difference in MEK-IIRL, LEK-IIRL or ?-EP-IIRL when compared with the acupuncture group. A high peak of the lymphocyte MEK-IR among the range of 10~(-10)—10~(-8)mol/L of MEK preincubation in the acupuncture group when compared with the control group. It suggests that electroacupuncture may enhance activity of the unbound MEK receptor.