ABSTRACT
Objective To research the metabolism and distribution ofsilybin in normal rats and liver injury model rats.Methods The normal rats group and immunity liver injury rat models were fed with the same dose ofsilybin capsule,and HPLC was used to determine the silybin concentration in biological samples in different time.Results The silybin concentration in the normal group in biological samples was higher than the model group at different time.In the normal group,the consequence of silybin concentration in each viscera distribution from top to bottom was liver＞kidneys＞plasma＞heart,while in the model group the fact was kidneys＞heart＞liver＞plasma.Conclusion The difference of metabolism and distribution of silybin in normal rats and liver damage model rats was obvious.
ABSTRACT
AIM: To investigate timed changes of bone histomorphometry parameters in normal rats of different months and to provide a control prove for research of drugs of antiosteoporosis. METHODS: Thirty two female rats at aged 4.5 months were divided by their weight ( 256.3 ? 25.3 g). Tetracycline derivates and calcein were subcutaneous injected to each rat on two separate occasions where labeled the sites of bone formation. All rats were sacrificed at 0, 30, 75 and 140 days. The undecalcified longitudinal proximal tibial metaphyseal sections were cut and stained for quantitative bone histomorphometriy. RESULTS: The cancellous bone mass increased slowly and then decreased, but there were no significant differences between 4.5 and 10 months. Bone mass kept relatively stable. Both bone formation and bone resorption increased first and then decreased, but there was a significant difference between 4.5 and 10 months, and bone turnover kept stable. CONCLUSION: The body weight and the bone mass parameters keep relatively stable in SD rats during the 4.5 to 10 months, and these rats can be selected as the models for the study of the drugs of antiosteoporosis.