ABSTRACT
Objective To investigate the expression level of nucleobindin-2 (NUCB2/Nesfatin-1) in nasopharyngeal carcinoma (NPC) patients, other patients with head and neck cancer, rhinitis patients, and healthy subjects in the serum, and evaluate the clinical application value of NUCB2/Nesfatin-1 combined with three items of Epstein-Barr (EB) virus (EA-IgA;VCA-IgA;Rta-IgG) in NPC diagnosis.Methods From Xiangya Hospital of Central South University during January 2017 to June 2017, nasopharyngeal carcinoma patients, other patients with head and neck cancer, rhinitis patients, and healthy subjects samples were 140 cases, respectively.The corresponding kits were used to detect nesfatin-1, EA-IgA, VCA-IgA, and Rta-IgG.Results The serum level of nesfatin-1 in NPC patients was the highest, and the difference was statistically significant compared to other groups.The serum levels of EA-IgA, VCA-IgA, and Rta-IgG were the highest in NPC patients, and were significantly different from those in other groups.Nesfatin-1 was significantly correlated with VCA-IgA and Rta-IgG.There was no significant correlation between nesfatin-1, EA-IgA and NPC staging;Multiple linear regression analysis was used to analyze the influencing factors of nesfatin-1, and the regression equation was Y =208.029 + 17.96X1 + 146.702X2 + 398.879X3 (X1:age;X2:VCA-IgA;X3:Rta-IgG;R2 =0.236).Receiver operating characteristic (ROC) curve analysis showed that nesfatin-I combined with VCA-IgA and Rta-IgG had the best efficiency in the diagnosis of NPC.In single index evaluation, nesfatin-1 has the lowest specificity, but the highest sensitivity.Conclusions The sensitivity of nesfatin-1 for NPC diagnosis is polar altitude, it can make up for the deficiency of EA-IgA, VCA-IgA, and Rta-IgG in diagnosing NPC.The combination of VCA-IgA and Rta-IgG can greatly improve the ability of diagnosing NPC.
ABSTRACT
Objective To evaluate the diagnostic value of combined detection of serum autoantibodies against against p53 and Bmi-1 in lung cancer (LC).Methods Serum levels of autoantibodies against p53 and Bmi-1 were measured by enzyme-linked immunosorbent assay (ELISA) in 92 patients with LC and 80 normal controls.The combined diagnostic value was evaluated with the receiver operating characteristic (ROC) curve.Results The serum levels of autoantibodies against p53 and Bmi-1 were significantly higher in LC than in normal controls (0.438 ±0.705 vs 0.045 ±0.035,Z =-7.667,P ＜0.01;0.117±0.061 vs 0.068 ±0.031,Z =-7.179,P ＜0.01).The levels of autoantibodies against p53 and Bmi-1 were not related to age,gender,pathological classification,lymph node status or tumor-node-metastasis (TNM) stage (P ＞ 0.05).The combined detection of two autoantibodies provided an enhanced sensitivity of 63.0％,a specificity of 91.2％ and an area under curve (AUC) of 0.881,which showed better diagnostic efficiency compared to individual autoantibodies.Conclusions Combined detection of autoantibodies against p53 and Bmi-1 shows good diagnostic value,which may aid diagnosis of LC.