ABSTRACT
Se estudió el comportamiento del volumen nuclear de los hepatocitos en 14 muestras del lóbulo medio de hígados de ratones isogénicos IOR que fueron sometidos a tres dosis de citosol de hígado regenerativo al inicio del experimento. Las muestras fueron tomadas durante ocho días con frecuencia de doce horas. El corte se dividió en zonas proximal, medial y distal al hilio. Se aplico el método morfométrico para la determinación del volumen nuclear de los hepatocitos a partir de su diámetro mayor y menor. Los resultados mostraron que los valores más altos del volumen nuclear esta en la zona proximal al hilio, aunque en todas las zonas existen diferencias significativas entre los valores obtenidos en la primera mitad de tiempo del experimento respecto a la parte final, con marcada orientación a decrecer lo que puede ser debido a la presencia de factores humorales estimulantes en el citosol regenerativo
We studied the behavior of the nuclear volume of hepatocytes in 14 samples of the middle lobe of isogenic rats liver IOR that were subjected to three doses of regenerating liver cytosol at the beginning of the experiment. Samples were taken during eight days with a frequency of twelve hours. The incision was divided into the proximal, medial and distal to the ileum zones. The Morphometric method was applied to determine the nuclear volume of hepatocytes from its major and minor diameter. The results showed that the highest values of the nuclear volume are located in the proximal zone to the ileum, although in all zones there are significant differences between the values obtained in the first half of the experiment in relation to the final part, with marked orientation to decrease what can be due to the presence of stimulant humoral factors in the regenerative cytosol
Subject(s)
Animals , Mice , Hepatocytes , Liver Regeneration , Cell Nucleus Size/physiology , Clinical TrialABSTRACT
This study evaluated the structure and the volume of interphase nuclei from root meristems of the genotypes of napier grass (Pennisetum purpureum), pearl millet (Pennisetum glaucum) and hybrids resultant of such breeding. In napier grass, nuclei were areticulate. Both pearl millet and the triploid hybrid had semi-reticulate nuclei; also, the hybrid presented a small proportion (6 percent) of areticulate nuclei. Pearl millet had the highest averages of nuclear dimensions, such as volume, diameter and radius, followed by the interspecific hybrid and napier grass. There was no intraspecific variation for the type of nuclear structure, which indicates this feature is important for cytotaxonomic studies involving the genus Pennisetum. Results demonstrated that chromatin organization in these nuclei was influenced by the number and size of chromosomes, affecting the nucleus volume in the analyzed taxa.
Avaliou-se, neste estudo, a estrutura e o volume de núcleos interfásicos de meristemas radiculares de genótipos do capim-elefante (Pennisetum purpureum), milheto (Pennisetum glaucum) e do híbrido resultante deste cruzamento. O capim-elefante apresentou núcleos do tipo arreticulado. Tanto no milheto como no híbrido triplóide os núcleos apresentaram-se semi-reticulados, sendo que, no híbrido, foi observada uma pequena proporção (6 por cento) de núcleos arreticulados. As maiores médias para as dimensões nucleares como volume nuclear, diâmetro e raio foram obtidas para o milheto, seguidas do híbrido interespecífico e capim-elefante. Não houve variação intraespecífica para tipo de estrutura nuclear indicando que essa característica tem relevância para estudos citotaxonômicos no gênero Pennisetum. Os resultados demonstraram que a organização da cromatina nesses núcleos foi influenciada pelo número e tamanho dos cromossomos e essa afetou o volume nuclear dos táxons analisados.
ABSTRACT
En el presente trabajo se pretendió determinar las variaciones que sobre las características morfométricas del riñón provoca la ingestión crónica de etanol en ratas adolescentes, para lo cual se utilizaron 42 ratas albinas machos de 30 días de nacidas con las cuales se conformaron dos grupos de 21 animales cada uno y con tiempos de tratamiento de tres y cinco meses y con estos, dos subgrupos: experimental y control. A las ratas experimentales se les suministró etanol a dosis de 5 g/kg de peso corporal mediante cánula intraesofágica. A las controles se les administró agua en lugar de etanol, en iguales condiciones. Se emplearon cortes histológicos coloreados con técnica PAS y se estudiaron las porciones contorneadas de los túbulos proximales y distales. Se calculó el área de sección transversal tubular y se midieron los volúmenes nucleares de las células de ambos túbulos. Se comprobó que las ratas experimentales mostraron volúmenes nucleares menores que las controles. El grupo experimental mostró valores de áreas de sección transversal de los túbulos renales mayores que los controles, siendo estos valores superiores en los túbulos proximales en las ratas de cinco meses, y los distales en las ratas de tres meses. Se concluyó que en la muestra estudiada el alcoholismo crónico iniciado en la adolescencia provocó variaciones morfométricas en los túbulos proximales y distales del riñón
Present paper allowed us to determine the variations exerted by the chronic ingestion of ethanol from the adolescence on the kidney morphometric features in 42 male albino rats of 30 days born divided into two groups of 21 animal each and with the treatment times of three and five months and with these 42 animals into two subgroups: one of experimental type and other as control. The experimental rats received ethanol at 5 g/kg doses according to the body weight using an intraesophageal cannula. The control ones received water instead of ethanol in similar conditions. We used histological sections stained with PAS technique to study the outlined portions of the proximal and distal tubules. The tubular transversal section area was estimated measuring the nuclear volumes of cells in both tubules. It was demonstrated that the experimental rats showed higher values of the transversal section of renal tubules higher than the control ones, where these values were superior in proximal tubules in the five months old rats and the distal ones in the three months old. We conclude that in study sample the chronic alcoholism started during adolescence provoked morphometric variations in proximal and distal tubules of kidney
Subject(s)
Animals , Rats , Alcoholism/complications , Cell Nucleus Size , Ethanol/adverse effects , Kidney/pathology , Kidney Tubules/anatomy & histologyABSTRACT
Ofloxacin presents an ample spectrum of antimicrobial action, including combating Mycobacterium leprae, and is currently employed as a substitute when the use of rifampicin is impossible. The objective of this work was to study alterations in testicular cell nuclei of suckling rats, whose dams were submitted to oral application of ofloxacin, and respective control groups. The method utilized was morphometry by the karyometric technique. The main structures observed in histological preparations of the testicles were interstitial cells, spermatogonias, and sustentacular cells. 10 Wistar rats were utilized, four treated and five controls, in the period of the first 25 days of life, whose dams received ofloxacin 12 mg/ Kg of body weight / day orally, before being killed on the 25th day after birth. The karyometric study of interstitial cells and spermatogonias revealed that there were no changes in the form of their nuclei (p > 0.05). Since sustentacular cell nuclei presented increased major diameter, minor diameter, mean geometric diameter, volume, area, volume/area ratio and perimeter, as well as an augmented and statistically different eccentricity (p < 0.05) in suckling pups whose dams were administered ofloxacin, the nuclei presented larger size and more elongated form. It was concluded that the sustentacular cells were more sensitive to the ofloxacin effect at the administered dose.
El ofloxacin presenta un amplio espectro de acción antimicrobiana, incluyendo el combate a.Mycobacterium leprae, y es frecuentemente empleado como un sustituto cuando el uso de la rifampicina es imposible. El objetivo del trabajo fue estudiar las alteraciones en el núcleo de las células testiculares en ratas que se encontraban amamantando y que fueron sometidas a la aplicación oral de ofloxacin. El método utilizado fue la técnica morfométrica de la cariometría. Las principales estructuras observadas en las preparaciones histológicas fueron las células intersticiales, espermatogenias y células sustentaculares. Se utilizaron 10 ratas Wistar, cinco fueron el grupo control y cinco sometidas al tratamiento, cuyas madres recibieron ofloxacin en dosis oral diaria de 12 mg/Kg de peso corporal los primeros 25 días de vida, para luego ser sacrificadas al día 25 después del nacimiento. El estudio cariométrico de las células intersticiales y de la espermatogénesis revelaron que no hubo cambios en la forma de sus núcleos (p > 0,05). Las células sustentaculares presentaron un incremento en su diámetro mayor, diámetro menor, diámetro geométrico promedio, volumen, área, razón volumen/área y perímetro, también hubo aumento con una diferencia de la excentricidad estadísticamente significativa (p < 0,05) en las crías amamantadas, a las cuales se les administró ofloxacin. Los núcleos presentaron un gran tamaño y una forma más alargada. Esto concluye que las células sustentaculares son más sensibles al efecto de la administración de ofloxacin.
Subject(s)
Animals , Male , Female , Pregnancy , Testis/drug effects , Ofloxacin/administration & dosage , Cell Nucleus/drug effects , Anti-Bacterial Agents/administration & dosage , Testis/cytology , Lactation , Ofloxacin/pharmacology , Rats, Wistar , Karyometry , Anti-Bacterial Agents/pharmacologyABSTRACT
Twenty cases of osteoblastic sarcoma were studied retrospectively with the stereometric method in order to provide objective morphometric data for the histological grading of the tumor.After standard fixation,embedding,sectioning and hematoxylin eosin staining,the average nuclear volume Vv was determined with the formula as follows;In the formula,Lo stands for the distance between 2 points resulting from the interception of a testing line through a nucleus.It was found that the Vv of osteoblastic sarcoma varied from 85 to 765?m3 and was consistent to the prognosis predicted with the Price's grading of sarcoma.It is believed that the determination of the stereomtric nuclear volume of osteoblastic sarcoma is able to provide an objective criterion for the prognosis of the tumor.