ABSTRACT
OBJECTIVE:To establish a method for the determining contents of 2 lignan components[dehydrodiisoeugenol and 2,3-dihydro-7-methoxy-2-(3,4-methylened ioxyphenyl)-3-methyl-5-(E)-propenyl-benzofuran(referred to“lignanoid 2”)]. METH-ODS:HPLC method was adopted. The column was Elite C18 with the mobile phase of water-methanol(gradient elution)at the flow rate of 1.0 ml/min;the detection wavelength was 225 nm and the column temperature was 30 ℃. The sample size was 20 μl. RE-SULTS:There was a good linear relationship between sample quantity and the peak area in the range of 0.202-2.02 μg(r=0.999 9) and 0.204-2.04 μg(r=0.999 9)for 2 lignan components. The RSD of precision,stability and repeatability tests were less than 2%with the average recovery of 101.54%(RSD=0.60%,n=6)and 99.43%(RSD=1.09%,n=6). CONCLUSIONS:The method is simple,sensitive and accurate,and can be used for the quantization determination of dehydrodiisoeugenol and lignanoid 2 in nut-meg-5.
ABSTRACT
Objective To develope a high performance liquid chromatograph ( HPLC ) method for simultaneous determination of nutmeg lignan and dehydrodiisoeugenol before and after processing of nutmeg. Methods The column was Diamonsil C18(250 mmí 4. 6 mm, 5 μm). The mobile phase was methanol-water in a gradient elution mode. The UV detection wave length was 274 nm. The column temperature was 25℃ . The flow rate was 1. 0 mL·min-1 . Results Nutmeg lignan and dehydrodiisoeugenol had a good linear correlation in ranges of 10. 24-61. 44 μg·mL-1(r=0. 999 6) and 3. 0-18. 0 μg·mL-1 (r=0.999 8), respectively. The average recovery rates were 97. 94% (RSD=2. 17%) and 97. 11% (RSD=2. 17%). Conclusion The method is simple, accurate, reproducible, and can be used for the simultaneous determination of nutmeg lignan and dehydrodiisoeugenol before and after the processing of nutmeg.
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[Objective]To measure the contents of Nutmeg ether,and its regularity varying with decoction time.[Method]RP-HPLC method was used.The chromatographic conditions were listed below Tigerkin-C18 column(150mm?4.6mm.5?m),amobine phase composed of metranol-0.025mol/L phosphoric acid(70∶30),a flow rate of 1mL ?min-1 and wave lengths was 282nm,column temperature was 25 ℃.[Results]In the range of 0.4?g ~2.0?g,the linear was very well between the content of Nutmeg ether and peak area.[Conclusion] The contents of Nutmeg ether in decoction reached the peak at 2.45min after Nutmeg ether was added in boiling water.
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Objective To optimize the most favorable extraction conditions of Nutmeg. Method With the volatile oils extracting ratio of Nutmeg as optimization targets, the extraction factors affected the volatile oils extracting ratio were optimized by uniform design. Result The optimum condition for extraction was as follows:medicinal materials which screen mesh were 4～10, being soaked in 14 times water for 1 hours and distilled for 6 hours with vapour. Conclusion The extraction process of optimization is simple and practicable, and can provide the test foundation for production.
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Objective To establish the fingerprints analysis of the methanol extracts of nutmeg,and study quality uniformity of nutmeg in different areas.Methods A ZORBAX EclipseXDB-C18 (4.6 mm?150 mm,5 ?m) column was used.The mobile phase consisted of methanol-acetonitrile-water (25∶35∶40),the flow rate was 1 mL/min,the column temperature was 30 ℃,the detective wavelength was 270 nm.Dehydrodiisoeugenol was used as reference compound.Results Fingerprint of nutmeg was established,consisted of l7 common peaks.The similarity of fingerprints was over 0.9.Conclusion The fingerprints of nutmeg in different areas have no differences.This method is accurate,reliable and provides a scientific basis for the quality control of nutmeg.