ABSTRACT
AIM:To study of effects of Panax notoginsengnoside(PnGL) on neutrophil(Neu) functions in carrageenin(Car) induced subcutaneous air-vesicle synovitis in rats and its mechanisms METHODS:Rat air-vesicle synovitis model was established with sc Car(25mg/kg) Neu count,the phagocytosis of Neu,the realease of O2 ?- from Neu and the level of Neu-i were measured with test tube diluting method,staphylococcus aureus phagocytosis method,cytochrome C reduction method and Fura-2 fluorescence technique respectively RESULTS:At 12h after Car used,Neu count of exudates elevated,phagocytosis rate and index of Neu and the release of O2 ?- from Neu increased obviously,suggesting that Neu was in an activated state Mea_nwhile,the level of Neu-i elevated significantly PnGL 60～240mg/kg ip,30min before and 6h after Car sc,reduced Neu count of exudates,inhibited phagocytosis functions and the increase of i level of Neu in a dose-dependent manner There was a significant correlation between the reduction of Neu-i and the inhibition of Neu phagocytosis functions and the release of O2 ?- from Neu Dexamethason had similar effects on Neu functions to PnGL,but with no effect on the level of Neu-i CONCLUSION:The reduction of Neu-i level and the subsequent inhibition of Neu functions is one of the important anti-inflammatory mechanisms of PnGL,which is different from that of Dex
ABSTRACT
OBJECTIVE:To study the scavenging action of Longyanshen on O2 ?- and ? OH.METHODS:Spectrophotometry (wavelength 322nm and 536nm)was used to detect the effects of Longyanshen upon O2 ?- and?OHRESULTS:Longyanshen in concentration of 30g/ml could reduce the level of?OH by 284%,and Longyanshen in concentrations of 15g/ml,30g/ml and 60g/ml could decrease the levels of O2 ?- by 441%,630% and 479% and inhibit the generation of O2 ?- by 489%,589%and 675% in vitro,respectivelyCONCLUSION:The results show that Longyanshen could scavenge and inhibit O2 ?- markedly(P