ABSTRACT
OBJECTIVE: To study the anti-obesity effect of crude extract of okra on nutrition-obese mice. METHODS: The fifty SPF ICR male mice were randomly divided into five groups: control(0.9% normal saline), model(0.9% normal saline), okra alcohol extraction high, medium and low dose group(400, 200, 100 mg·kg-1). In addition to the mice in control group, the mice in other four groups were given high fat diet to make the model of obese mice, after oral administration for 4 weeks, eyeball blood of the mice was taken to measure the contents of serum triglyceride(TG), total cholesterol(TC), leptin(LEP), adiponectin(ADP), high density lipoprotein(HDL-C), and low density lipoprotein(LDL-C). Then mice were sacrificed to take their liver tissue and fat to detect the expression of SREBP-1 and FAS mRNA by RT-PCR. RESULTS: Compared with the model, the okra alcohol extract could effectively decrease the contents of TC, TG, LDL-C and LEP, increase the contents of HDL-C and ADP(P<0.01, P<0.05), improve the dyslipidemia, and regulate the secretion of protein in adipose tissue, to achieve the therapeutic effect on obese mice. The expressions of SREBP-1 and FAS mRNA were also significantly inhibited by crude extract of okra. CONCLUSION: The results show that treatment of crude extract of okra on obese mice is associated with the expressions of SREBP-1 and FAS.
ABSTRACT
OBJECTIVE@#To explore the effect of okra extract on gestational diabetes mellitus (GDM) rats and its probable molecular mechanism.@*METHODS@#A total of 30 female SD rats were caged with male rats for pregnancy, 27 pregnant rats were obtained and weighed. The pregnant rats were equally randomized into the control group, GDM group and intervention group. Once the pregnancy was verified, GDM group and intervention group were given 45 mg/kg streptozotocin by peritoneal injection for inducing GDM, control group was given equal volume of citrate buffer. Once the model was established successfully, intervention group was administered orally the solution containing 200 mg/kg/d okra extract, the other groups were given the diet and water only. On the 19th day of pregnancy, the blood samples and fetal rats of all groups were collected, fetal rats weight and placental weight was recorded and the serum glucose, lipids, serum insulin and C-peptide of pregnant rats before the delivery were determined.@*RESULTS@#The pregnant rats weight before the delivery, fetal rats weight and placental weight of GDM group were lower than control group and intervention group (P 0.05). Antioxidant enzymes levels of GDM group in liver and pancreas tissues were lower than the other groups, and after treatment of okra extract, antioxidant enzymes levels in liver and pancreas tissues were equivalent to control group (P > 0.05).@*CONCLUSIONS@#Okra extract, rich in antioxidant substances, could avoid the excessive consuming of antioxidant enzymes, then, suppresses the oxidative stress and insulin resistance, thereby improving blood glucose level of GDM rats.
ABSTRACT
Objective: To explore the effect of okra extract on gestational diabetes mellitus (GDM) rats and its probable molecular mechanism. Methods: A total of 30 female SD rats were caged with male rats for pregnancy, 27 pregnant rats were obtained and weighed. The pregnant rats were equally randomized into the control group, GDM group and intervention group. Once the pregnancy was verified, GDM group and intervention group were given 45 mg/kg streptozotocin by peritoneal injection for inducing GDM, control group was given equal volume of citrate buffer. Once the model was established successfully, intervention group was administered orally the solution containing 200 mg/kg/d okra extract, the other groups were given the diet and water only. On the 19th day of pregnancy, the blood samples and fetal rats of all groups were collected, fetal rats weight and placental weight was recorded and the serum glucose, lipids, serum insulin and C-peptide of pregnant rats before the delivery were determined. Results: The pregnant rats weight before the delivery, fetal rats weight and placental weight of GDM group were lower than control group and intervention group (P 0.05). Antioxidant enzymes levels of GDM group in liver and pancreas tissues were lower than the other groups, and after treatment of okra extract, antioxidant enzymes levels in liver and pancreas tissues were equivalent to control group (P > 0.05). Conclusions: Okra extract, rich in antioxidant substances, could avoid the excessive consuming of antioxidant enzymes, then, suppresses the oxidative stress and insulin resistance, thereby improving blood glucose level of GDM rats.