Microbial biostimulants as alternatives for the rooting of olive tree cuttings

Currently, southern Minas Gerais (MG) state is an important producer of different olive tree (Olea europaea L.) cultivars because, in this region, the plants can differentiate the buds to produce flowers and fruit. To stimulate the rooting of cuttings, the synthetic hormone indole-3-butyric acid (IBA) at a concentration of 3 g L−1 is used commercially. However, few studies have investigated arbuscular mycorrhizal fungi (AMF), isolated or combined with rhizobacteria, as a biotechnological tool to produce hormones that function in the rooting of olive tree cuttings. The aim of this study was to evaluate the capacity of different AMF species (Rhizophagus clarus, Gigaspora rosea, or Acaulospora scrobiculata), combined or not with IBA or rhizobacteria, to promote the rooting of three olive tree cuttings (Arbequina, Grappolo 541, and Maria da Fé) with potential for cultivation in this region. For this, three experiments were conducted at the Experimental Farm of EPAMIG in Maria da Fé (MG), and the rooting potential of the olive tree cuttings inoculated with I) AMF, II) AMF combined with increasing doses of IBA, and III) AMF combined with three isolates of rhizobacteria was evaluated. The inoculation of olive tree cuttings of cultivars Arbequina, Grappolo 541, and Maria da Fé with Rhizophagus clarus, Gigaspora rosea, or Acaulospora scrobiculata combined or not with IBA or rhizobacteria did not significantly promote rooting. Alternative forms of rooting olive tree cuttings are still a challenge, and further studies for standardizing methodologies and experimental conditions are required.

Evaluation comparative de l'activité anti-oxydante des extraits éthanoliques des feuilles d'Olea europaea L. de l'Est Algérien / Comparative evaluation of antioxidant activity of ethanolic extracts from leaves of Olea europaea L. from Eastern Algeria

Introduction: Objectif-Dans les dernières décennies il y a eu un intérêt croissant pour l'étude des plantes médicinales et leur utilisation traditionnelle pour le traitement de diverses maladies. L'olivier ou Olea europaea L. constitue une entité indissociable des peuples méditerranéens. Cette plante appartient à la grande famille des oleaceae. L'objectif de cette étude a été d'évaluer l'activité anti-oxydante des extraits éthanoliques de quatre variétés sauvages de la plante O.europaea des régions de l'Est algérien : Batna et Mila (hautes-plateaux), Biskra (Sahara) et Skikda (ville côtière). Matériels et méthodes - L'évaluation de l'activité anti-oxydante in vitro des extraits éthanoliques de la plante O.europaea a été réalisée par différentes méthodes, notamment le piégeage du radical libre DPPH• et le pouvoir réducteur des ions ferriques. Résultats : L'analyse quantitative des extraits éthanoliques des feuilles de la plante O.europaea a révélé que l'extrait de Mila était plus riche (420.36 mg GAE/g MS), suivi de celui de Batna (396.84 mg GAE/g MS), puis celui de Biskra (de 380.69 mg GAE/g MS) et enfin de l'extrait de Skikda (368.45 mg GAE/g MS). Les résultats de l'activité antiradicalaire des extraits éthanoliques ont montré que ceux de Batna et Mila possédaient le pouvoir le plus important. Conclusion -Les résultats obtenus confirment favorablement l'utilisation des feuilles d'O.europaea en médecine traditionnelle algérienne pour le traitement de diverses maladies.

Introduction-Objective-In recent decades the study of medicinal proprieties of Olea europaea showed an increase interest of medicinal plants and their traditional use for the treatment of various diseases. The olive tree or Olea europaea L. is an inseparable entity of the Mediterranean people. This plant belongs to the large family of oleaceae. The purpose of this study was to assess the antioxidant activity of the ethanolic extracts from four wild varieties of O.europea in the eastern regions of Algeria: Batna and Mila (highlands), Biskra (Sahara) and Skikda (coastal city). Material and methods - The evaluation of the antioxidant activity in vitro of the ethanolic extracts of O.europaea plant was carried out by various methods, in particular, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging effect and ferric reducing antioxidant power (FRAP). Results -Quantitative analysis of ethanolic extracts from the leaves of the plant O.europaea revealed that Mila's extract was richer (420.36 mg GAE / g DM), followed by that of Batna (396.84 mg GAE / g DM), then that of Biskra (380.69 mg GAE / g MS) and finally by Skikda extract (368.45 mg GAE / g MS). The results of DPPH free radical scavenging of the ethanolic extracts showed that those of Batna and Mila had the most important power. Conclusion - The obtained results favorably confirm the use of O.europaea leaves in traditional Algerian medicine for the treatment of various diseases.

Follow-up of a Composite Endomycorrhizal Inoculum in the Rhizosphere of Olive Plants, Analysis after 42 Months of Culture

Endomycorrhizal inoculum, consisting of more than 26 mycorrhizal species, has been followed over time at the rhizosphere of olive trees. The evolution during time of this endomycorrhizal inoculum at the rhizosphere of plants has been discussed in this study. After 42 months, 45 species were isolated from the rhizosphere of inoculated olive plants. These species belong to 6 genera (Glomus, Acaulospora, Gigaspora; Scutellospora; Pacispora and Entrophospora), from these genera, Glomus was the most dominant (40%) followed by the Acaulospora (30%). Glomus constrictum and G. intraradices were the most abundant species, their frequency are respectively 17% and 15%. In comparison between detected species, those of primary inoculum and those recovered after 30 months, 36 endomycorrhizal species appeared and 14 species disappeared, but four species G. clarum, G. intraradices, G. mossea and G. versiforme have been able to maintain their status and stability of multiplication in the rhizosphere of olive plants.

Separation and preparation of polyphenols from leaves of Olea europaea by preparative medium pressure liquid chromatography / 中草药

Objective: To study the material basis of the anti-diabetic effective fraction in the leaves of Olea europaea. Methods: Preparative medium pressure liquid chromatography (PMPLC) was used for the separation and purification of the compounds. Their structures were elucidated by spectral analyses. Results: The structures of the compounds were identified by various spectroscopic methods combined with the data from some published literatures. There were five compounds which were extracted from the anti-diabetic effective fraction in the leaves of O. europaea and identified as decaffeoylverbascoside (1), luteolin-7-O-β-D-glucoside (2), 6″-O-β-D-glucopyranosyl oleuropein (3), oleuroside (4), and oleuropein (5). Additionally, decaffeoylverbascoside is isolated from this plant for the first time and the purities of the five compounds were all above 90% by HPLC detection. Conclusion: This research provides technological support for the development of new drug based on the leaves of O. europaea.

Desenvolvimento vegetativo de duas cultivares de oliveira na fase de muda / Vegetative development of two olive cultivars in seedling phase

O desenvolvimento de plantas, influenciado pela temperatura do ar, pode ser estimado através do conceito de filocrono, definido como o intervalo de tempo entre o surgimento sucessivo de folhas em uma haste, sendo o tempo expresso em °C dia e o filocrono em °C dia folha-1. O objetivo deste estudo foi avaliar o desenvolvimento de duas cultivares de oliveira durante a fase de muda, em diferentes épocas de aclimatação, utilizando o conceito de filocrono. O experimento foi conduzido na Fazenda Experimental da Epamig, em Maria da Fé, sul de Minas Gerais, Brasil. O delineamento utilizado foi o inteiramente casualizado em esquema fatorial 2x5, com duas cultivares ('MGS ASC315' e 'Arbequina'), cinco épocas de aclimatação (maio, junho, julho, agosto e setembro de 2010) e 15 repetições. O filocrono foi estimado pelo inverso do coeficiente angular da regressão linear entre o número de folhas emitidas (NF) e a soma térmica acumulada (STa). O filocrono em 'MGS ASC315' foi de 14,7°C dia folha-1 (época 1), 31,7°C dia folha-1 (média das épocas 2, 3 e 4) e 60,2°C dia folha-1 (época 5). O filocrono em 'Arbequina' foi de 17,3°C dia folha-1 (média das cinco épocas de aclimatação, uma vez que não apresentou diferença significativa entre as épocas). Nas épocas de aclimatação 3, 4 e 5, houve diferença do filocrono entre as cultivares, sendo que o menor filocrono foi para a cultivar 'Arbequina'. Nas épocas 1 e 2, não houve diferenças significativas entre as cultivares.

Plants development, influenced by air temperature, can be estimated through the concept of phyllochron, defined as the time interval between the appearance of successive leaves on a stem, being the time expressed in °C day and the phyllochron in °C day leaf-1. The objective of this study was to evaluate the development of two olive cultivars during the seedling phase at different acclimation dates, using the concept of phyllochron. The experiment was carried in the experimental area of the Agricultural Research Corporation of Minas Gerais, Maria da Fé, south of Minas Gerais, Brazil. The experimental design was completely randomized with two cultivars (MGS 'ASC315' and 'Arbequina'), five acclimation date (May, June, July, August and September 2010) and 15 repetitions. The phyllochron was estimated by the inverse of the slope of the linear regression between leaves number (NF) and accumulated thermal time (STa). The phyllochron in 'MGS ASC315' was 14,7°C day leaf-1 (date 1), 31,7°C day leaf-1 (average of dates 2, 3 and 4) and 60,2°C day leaf-1(date 5). The phyllochron in 'Arbequina' was 17,3°C day leaf-1 (average of five acclimation dates, due not statistically difference among dates). In acclimation dates 3, 4 and 5, not statistically difference between cultivars, and the smallest phyllochron for 'Arbequina'. In acclimation dates 1 and 2, were not statistically different between cultivars.

Polyphenols from leaves of Olea europaea / 中草药

Objective: To study the polyphenols from the leaves of Olea europaea. Methods: The polyphenols from ethyl acetate fraction were separated and purified by silica chromatographic method and identified by spectroscopic analysis. Results: In combination with the data from literatures, the structure of 15 polyphenols had been elucidated on the basis of various spectroscopic methods, including tyrosol (1), hydroxytyrosol (2), hydroxytyrosol acetate (3), 1H-2-benzopyran-6,7-diol (4), 3,4-dihydroxyl-benzoic acid (5), 3-methoxyl-4-hydroxyl benzoic acid (6), isorhamnetin (7), eriodictyol (8), taxifolin (9), quercetin (10), luteolin (11), ligstroside (12), oleuropein (13), apigenin-7-O-glucoside (14), and luteolin-7-O-glucoside (15). Conclusion: Compounds 4-9 are isolated from this plant for the first time.

Enraizamento de estacas de oliveira submetidas a aplicação de fertilizantes orgânicos e AIB / Rooting of olive tree cuttings using organic fertilizations and IBA

Estacas semilenhosas de oliveiras 'Ascolano 315', foram preparadas com aproximadamente 12 cm de comprimento e quatro folhas, sendo, em seguida, tratadas, ou não, com 3000 mg L-1 de AIB por cinco segundos O experimento foi conduzido em casa de vegetação, com sistema de nebulização intermitente, sendo as estacas colocadas em bancadas de propagação, contendo a perlita como substrato. Antes do plantio das estacas, foram aplicados os fertilizantes orgânicos Biofertilizante e Nippoterra, nas dosagens 0, 20, 40 e 60 mL L-1. Os produtos foram aplicados nas parcelas experimentais com regador manual, em dosagem única. Passados 58 dias, foi mensurada a porcentagem de estacas com calo, enraizadas, enraizadas e/ou com calo, número médio de raízes e comprimento médio das raízes. Concluiu-se que a dosagem de 40 mL L-1 de Biofertilizante propiciou melhores resultados, com a utilização de AIB.

Semi hardwood cutting was colletecd in the medium portion of the 'Ascolano 315' olive trees, prepared with 12 cm in length and four leaves, treated or not with 3000 mg L-1 of IBA by five seconds. The experiment was conducted in a greenhouse, with a system of intermittent nebulization. The cutting was placed in propagation supports, containing perlite as a substrate. Before the plantation of the cutting, organic fertilizers Biofertilizante and Nippoterra were applied in the proportion: 0, 20, 40 and 60 mL L-1. The products were applied in the experimental portions in a single dose. After 58 days, the percentage of cutting with callus, taken root, taken root and/or with callus, average number of roots and average length of the roots were evaluated. The 40 mL L-1 of Biofertilizante gave the best results, with IBA.

Estaquia de oliveira em diferentes épocas, substratos e doses de AIB diluído em NaOH e álcool / Rooted stem cutting of the olive tree in different times, substrates and doses of IBA dilluted in NaOH and alcohol

A estaquia é um método de propagação muito utilizado para a oliveira. Sua viabilidade depende da capacidade de formação de raízes adventícias de cada espécie. Contudo é necessário estudar as diferentes características desse processo. Objetivou-se, no presente trabalho avaliar o efeito de concentrações de AIB, do veículo de diluição, do substrato e época de coleta, no enraizamento de estacas semilenhosas de oliveira 'Ascolano 315'. Foram coletados ramos medianos de plantas matrizes localizadas na unidade da Epamig, em Maria da Fé-MG, e preparadas as estacas com, aproximadamente, 12 cm de comprimento, com 4 a 6 internódios, mantendo na região apical quatro folhas. O delineamento utilizado foi o inteiramente casualizado, em esquema fatorial 4 x 2 x 2 x 5, com quatro repetições e quinze estacas por parcela. O experimento foi conduzido em câmara úmida, com fornecimento de calor no fundo das bandejas de enraizamento. Após a instalação do experimento, foi realizado um tratamento com solução de oxicloreto de cobre a 3 por cento, para prevenir eventuais ataques de fungos e, a cada sete dias, a câmara foi aberta por alguns minutos para renovação do ar e irrigação, para manutenção da umidade. As avaliações foram realizadas 65 dias após a estaquia de cada época de coleta. As estacas coletadas em abril e tratadas com 3000 mg.L-1 apresentaram maior porcentagem de enraizamento e número médio de raízes. O substrato perlita + vermiculita (1:1 v/v) incrementou o comprimento médio das raízes. A diluição do AIB em NaHO ou álcool + água não diferiu.

The rooted stem cutting is a method highly used for the propagation of olive tree. Its viability depends on the capacity of each species to form adventitious roots. However, it is necessary to study the different characteristics of this process. The objective of the present work was to evaluate the effect of IBA concentrations, the dilution carrier, the substrate and the times of collection on the rooting of the semi-hardwood stem cuttings of olive tree "Ascolano 315'. Median branches of stock plants located at the EPAMIG unit in Maria da Fé-MG have been collected and cuttings about 12 centimeters in length with 4 to 6 units of internodes were prepared, with four leaves being kept in the apical region. The experimental design was the completely randomized in a factorial scheme 4 x 2 x 2 x 5, with four replications and fifteen cuttings per plot. The experiment was carried out in a moist chamber with the supply of heat at the bottom of the rooting trays. After the experiment was established, a treatment was accomplished with a solution of 3 percent copper oxychloride to prevent occasional fungal attacks and every seven days, the chamber was opened for a few minutes for air renewal and irrigation for maintenance of moisture. The evaluations were accomplished 65 days after the staking of cuttings from each collection time. The cuttings collected in April and treated with 3,000 mg.L-1 IBA showed increased percentage of rooting and average number of roots. The perlite + vermiculite (1:1 v/v) enhanced the average length of the roots. The dilution of IBA in NaOH or alcohol + water did not present difference.